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The map label for this gene is nccB [H]

Identifier: 121611735

GI number: 121611735

Start: 5343351

End: 5344529

Strand: Direct

Name: nccB [H]

Synonym: Veis_4833

Alternate gene names: 121611735

Gene position: 5343351-5344529 (Clockwise)

Preceding gene: 121611734

Following gene: 121611736

Centisome position: 95.99

GC content: 71.08

Gene sequence:

>1179_bases
ATGCAATGGATATGGCAGATGCCCTCTTTCAAAATCTCCTGCGCCGCTCTGGCCGCCGCCTTGGCGCTGGCTGCGGCCGG
CGCGTTGCTGGTGACCGGCGCATCGCGCGCGGCCAACGAGTCTGAGGCAGGCCCGGCCCGGCCGGCGCTCACGGTGACTC
AGGCGCAACCGCAGCGCAGCACCATCGCGCTGCGGCTGGCAGCCAACGGCGATGTGGCCGCCTGGCAAGAGGCCAGCATT
GGCGCCGAAAGCCATGGCCTGCGCCTGACCGAGGTGCTGGTCAATGTGGGTGACAGGGTTCAGGCGGGCCAAGTGCTGGC
CCGCTTTGCGACCGACACCGTGCAGGCCGATGTCGCCCAGGCGCGCGCCAGCCTGCTCGAAGCCCAGGCCCATGCCGCCG
AGGACCAGGCCAACGCCGACCGGGCCCGCTCGCTACAGGCCACGGGCGCGTTGAGCGTGCAGCAAATCGCGCAGTTGACC
ACGGCCGACAAGACCGCGCAGGCCCGGGTGCAGGCCGCGCAGGCGGGCCTGGATGCGCAGTTGCTGCGCCTGCGCCAGAC
CCGGGTGCTGGCCCCCGACGGCGGCGTGATCTCGGCGCGCACGGCCACCGTGGGCGCCGTGGTGAGCGCAGGCACCGAGC
TGTTTCGCATGTTGCGCAAGGGCCGGCTGGAATGGCGCGCCGAGGTCACCGCCGCCGAACTGCACCGCATCACGCCGGGC
ACCCGGGTCAGCGTCACGACCGCCGGCGGCGCGGTGCTCGAGGGCCGGGTGCGCATGTTGGCCCCGACGGTCGATCCGCA
CACGCGCAATGCGTTGGTCTATGTCGACCTGCCGGCCCACGCGCAACTGCGCGCCGGCATGTTTGCCCGCGGCGAGTTCG
ACCTGGGCAGCAGTGCCGCGCTCACGGTGCCGCAGCAGGCGCTGGTGCTGCGCGATGGTTTCTCTTACGTGTTCGTGCTC
GGCGCCGATCAGCGCGTGCAGCAGCGCAAGGTGCAAACCGGCCGCCGCGTGGCCGACCGGGTGGAACTGCTCGCGGGCCT
GGACGCGCAGTCCTGGATCGCCGTGCAGGGCGCGGGTTTTCTCAACGACGGCGACCTGGTGCGCGTGGTCGCCTCCCAGG
ATTCCACGCAGAAAACACCGGTAGCGCAGGCAAAGCACGCGCCTTCAGCCATCCATTGA

Upstream 100 bases:

>100_bases
CCAAGGTATGATTCGGCGACATCCGGCGATGGGCCGCCGCCCGGCCCTCCGATCGCCGGATGCGAACTGCGGTTTTCAGG
CCGAATGAATCCATCACCCG

Downstream 100 bases:

>100_bases
AGAGCATTCTTGATGAATGCCTCCGCCTGGGCCATCAGGAACCCGATCCCTGCGCTGATGCTGTTCGTGCTGCTGGGCTT
TGGCGGCCTGCTGGCGTTCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MQWIWQMPSFKISCAALAAALALAAAGALLVTGASRAANESEAGPARPALTVTQAQPQRSTIALRLAANGDVAAWQEASI
GAESHGLRLTEVLVNVGDRVQAGQVLARFATDTVQADVAQARASLLEAQAHAAEDQANADRARSLQATGALSVQQIAQLT
TADKTAQARVQAAQAGLDAQLLRLRQTRVLAPDGGVISARTATVGAVVSAGTELFRMLRKGRLEWRAEVTAAELHRITPG
TRVSVTTAGGAVLEGRVRMLAPTVDPHTRNALVYVDLPAHAQLRAGMFARGEFDLGSSAALTVPQQALVLRDGFSYVFVL
GADQRVQQRKVQTGRRVADRVELLAGLDAQSWIAVQGAGFLNDGDLVRVVASQDSTQKTPVAQAKHAPSAIH

Sequences:

>Translated_392_residues
MQWIWQMPSFKISCAALAAALALAAAGALLVTGASRAANESEAGPARPALTVTQAQPQRSTIALRLAANGDVAAWQEASI
GAESHGLRLTEVLVNVGDRVQAGQVLARFATDTVQADVAQARASLLEAQAHAAEDQANADRARSLQATGALSVQQIAQLT
TADKTAQARVQAAQAGLDAQLLRLRQTRVLAPDGGVISARTATVGAVVSAGTELFRMLRKGRLEWRAEVTAAELHRITPG
TRVSVTTAGGAVLEGRVRMLAPTVDPHTRNALVYVDLPAHAQLRAGMFARGEFDLGSSAALTVPQQALVLRDGFSYVFVL
GADQRVQQRKVQTGRRVADRVELLAGLDAQSWIAVQGAGFLNDGDLVRVVASQDSTQKTPVAQAKHAPSAIH
>Mature_392_residues
MQWIWQMPSFKISCAALAAALALAAAGALLVTGASRAANESEAGPARPALTVTQAQPQRSTIALRLAANGDVAAWQEASI
GAESHGLRLTEVLVNVGDRVQAGQVLARFATDTVQADVAQARASLLEAQAHAAEDQANADRARSLQATGALSVQQIAQLT
TADKTAQARVQAAQAGLDAQLLRLRQTRVLAPDGGVISARTATVGAVVSAGTELFRMLRKGRLEWRAEVTAAELHRITPG
TRVSVTTAGGAVLEGRVRMLAPTVDPHTRNALVYVDLPAHAQLRAGMFARGEFDLGSSAALTVPQQALVLRDGFSYVFVL
GADQRVQQRKVQTGRRVADRVELLAGLDAQSWIAVQGAGFLNDGDLVRVVASQDSTQKTPVAQAKHAPSAIH

Specific function: Component of the NCC cation efflux system that confers resistance to nickel, cobalt and cadmium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=23.8219895287958, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081796, Length=317, Percent_Identity=25.5520504731861, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41162; Mature: 41162

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWIWQMPSFKISCAALAAALALAAAGALLVTGASRAANESEAGPARPALTVTQAQPQRS
CCCEECCCCCEEHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCEEEEECCCCCCC
TIALRLAANGDVAAWQEASIGAESHGLRLTEVLVNVGDRVQAGQVLARFATDTVQADVAQ
EEEEEEECCCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ARASLLEAQAHAAEDQANADRARSLQATGALSVQQIAQLTTADKTAQARVQAAQAGLDAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
LLRLRQTRVLAPDGGVISARTATVGAVVSAGTELFRMLRKGRLEWRAEVTAAELHRITPG
HHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
TRVSVTTAGGAVLEGRVRMLAPTVDPHTRNALVYVDLPAHAQLRAGMFARGEFDLGSSAA
CEEEEEECCCEEEECCEEEEECCCCCCCCCEEEEEECCCCHHHHCCEEECCCCCCCCCCE
LTVPQQALVLRDGFSYVFVLGADQRVQQRKVQTGRRVADRVELLAGLDAQSWIAVQGAGF
EECCHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LNDGDLVRVVASQDSTQKTPVAQAKHAPSAIH
CCCCCEEEEEECCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MQWIWQMPSFKISCAALAAALALAAAGALLVTGASRAANESEAGPARPALTVTQAQPQRS
CCCEECCCCCEEHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCEEEEECCCCCCC
TIALRLAANGDVAAWQEASIGAESHGLRLTEVLVNVGDRVQAGQVLARFATDTVQADVAQ
EEEEEEECCCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ARASLLEAQAHAAEDQANADRARSLQATGALSVQQIAQLTTADKTAQARVQAAQAGLDAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHH
LLRLRQTRVLAPDGGVISARTATVGAVVSAGTELFRMLRKGRLEWRAEVTAAELHRITPG
HHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCC
TRVSVTTAGGAVLEGRVRMLAPTVDPHTRNALVYVDLPAHAQLRAGMFARGEFDLGSSAA
CEEEEEECCCEEEECCEEEEECCCCCCCCCEEEEEECCCCHHHHCCEEECCCCCCCCCCE
LTVPQQALVLRDGFSYVFVLGADQRVQQRKVQTGRRVADRVELLAGLDAQSWIAVQGAGF
EECCHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LNDGDLVRVVASQDSTQKTPVAQAKHAPSAIH
CCCCCEEEEEECCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]