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The map label for this gene is rsmE [H]

Identifier: 121611717

GI number: 121611717

Start: 5316236

End: 5317057

Strand: Direct

Name: rsmE [H]

Synonym: Veis_4814

Alternate gene names: 121611717

Gene position: 5316236-5317057 (Clockwise)

Preceding gene: 121611716

Following gene: 121611726

Centisome position: 95.5

GC content: 72.87

Gene sequence:

>822_bases
ATGACCCCGATGCCGCGCTTTCACTGCCCCAGTCCATTGGCCACGGGCGCCGAGCTCGACCTGCCGGCCCGCGTGGCGCG
CCATGTGCAGGTGCTGCGCTTGCAGCCGGGCGACGGCATCACGCTGTTCCATGGCGGCCAGGGCGGCGAGTTTGACGCCA
CCGTGCTGCGCATGGGCCGCAGCGACGTGCGCGTGCAACTGGGTGCCCACCACCGCATCGAGCGCGAAGCCCCCCGGGCC
GTGCACCTGCTCGCCGGCATCATGGCCAACGAGCGCATGGACTGGCTGGTGGAAAAAGCCACCGAACTGGGCGCCGCCAG
CATCACGCCGCTGCTGACCGAGCGCAGCGTGCTCAAGCTCAGGGGCGAGCGCGCCGACAAGAAAATCGCCCACTGGCAGG
CCGTGGCCGTGGCCGCCTGCGAGCAATGCGGGCGCAACCGCGTGCCCCCGGTGCACCCGGCCACGGCCCTGGCGCACTGG
GTGCGCGCCCAGGCGGCGGCCTGCGTCCAGCAAACTGCGCTGCGCCTGCTGCTGGCGCCAGGCCCCGGCGCCGCACCGCT
GCACAGCGCTGCGGCGGCCGATCGCGGCCTGCGGCCTGCGGCCGATCGCGACGAACGCCCCCTGTACATCCTCTCAGGCG
CCGAAGGCGGTCTGAGCAGCGCCGAGCAAGACCTGGCCCGGCAGCACGGTTTTACCCCCGTCACGCTAGGCCCGCGCGTG
CTGCGCGCCGAAACCGCAGCGCTGGCGGCGCTGGCGGCCCTCACGCTGGCCAGCCTCAGCCCTTCAATGCCAGCGTGGAA
ATGCCCGGAACCGCCGCAATGA

Upstream 100 bases:

>100_bases
GGGCCTGGCGTATGGCCGGGTATAGCGCTGGCACGATGCGGCATCGCATCGGCCACCGGCGCATGTCCGGTACTGCGCAG
GAAGCGGCGCATGGCTTGGC

Downstream 100 bases:

>100_bases
CCACGACCCCGGCCAGCAAGGCCAGCAGGTAAGGCCAAATCGAGCGCAAGGCTTCATCCGGCTTGACCTCGCCGATACGG
CAGGCAATATAGAACCCGAC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTPMPRFHCPSPLATGAELDLPARVARHVQVLRLQPGDGITLFHGGQGGEFDATVLRMGRSDVRVQLGAHHRIEREAPRA
VHLLAGIMANERMDWLVEKATELGAASITPLLTERSVLKLRGERADKKIAHWQAVAVAACEQCGRNRVPPVHPATALAHW
VRAQAAACVQQTALRLLLAPGPGAAPLHSAAAADRGLRPAADRDERPLYILSGAEGGLSSAEQDLARQHGFTPVTLGPRV
LRAETAALAALAALTLASLSPSMPAWKCPEPPQ

Sequences:

>Translated_273_residues
MTPMPRFHCPSPLATGAELDLPARVARHVQVLRLQPGDGITLFHGGQGGEFDATVLRMGRSDVRVQLGAHHRIEREAPRA
VHLLAGIMANERMDWLVEKATELGAASITPLLTERSVLKLRGERADKKIAHWQAVAVAACEQCGRNRVPPVHPATALAHW
VRAQAAACVQQTALRLLLAPGPGAAPLHSAAAADRGLRPAADRDERPLYILSGAEGGLSSAEQDLARQHGFTPVTLGPRV
LRAETAALAALAALTLASLSPSMPAWKCPEPPQ
>Mature_272_residues
TPMPRFHCPSPLATGAELDLPARVARHVQVLRLQPGDGITLFHGGQGGEFDATVLRMGRSDVRVQLGAHHRIEREAPRAV
HLLAGIMANERMDWLVEKATELGAASITPLLTERSVLKLRGERADKKIAHWQAVAVAACEQCGRNRVPPVHPATALAHWV
RAQAAACVQQTALRLLLAPGPGAAPLHSAAAADRGLRPAADRDERPLYILSGAEGGLSSAEQDLARQHGFTPVTLGPRVL
RAETAALAALAALTLASLSPSMPAWKCPEPPQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=255, Percent_Identity=37.6470588235294, Blast_Score=157, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29053; Mature: 28922

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPMPRFHCPSPLATGAELDLPARVARHVQVLRLQPGDGITLFHGGQGGEFDATVLRMGR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEECCCCCCHHHHHHHHCC
SDVRVQLGAHHRIEREAPRAVHLLAGIMANERMDWLVEKATELGAASITPLLTERSVLKL
CCEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RGERADKKIAHWQAVAVAACEQCGRNRVPPVHPATALAHWVRAQAAACVQQTALRLLLAP
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
GPGAAPLHSAAAADRGLRPAADRDERPLYILSGAEGGLSSAEQDLARQHGFTPVTLGPRV
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECCCHH
LRAETAALAALAALTLASLSPSMPAWKCPEPPQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPMPRFHCPSPLATGAELDLPARVARHVQVLRLQPGDGITLFHGGQGGEFDATVLRMGR
CCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEECCCCCCHHHHHHHHCC
SDVRVQLGAHHRIEREAPRAVHLLAGIMANERMDWLVEKATELGAASITPLLTERSVLKL
CCEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RGERADKKIAHWQAVAVAACEQCGRNRVPPVHPATALAHWVRAQAAACVQQTALRLLLAP
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
GPGAAPLHSAAAADRGLRPAADRDERPLYILSGAEGGLSSAEQDLARQHGFTPVTLGPRV
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECCCHH
LRAETAALAALAALTLASLSPSMPAWKCPEPPQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]