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The map label for this gene is queF

Identifier: 121611693

GI number: 121611693

Start: 5290615

End: 5291460

Strand: Direct

Name: queF

Synonym: Veis_4790

Alternate gene names: 121611693

Gene position: 5290615-5291460 (Clockwise)

Preceding gene: 121611692

Following gene: 121611694

Centisome position: 95.04

GC content: 68.32

Gene sequence:

>846_bases
ATGAACCCGCCAGAGTCATCGCCGTTGGGCAAGGCCTCCGCCTGTGTCGACCAGTACGACGCCACGCTGCTGTTTCCGAT
AGCGCGCGCGGACCAGCGGGCCGAGCTTGGCATCAGCGGCGCGGCGCCCTTTTTCGGGGCCGACCTGTGGACGGCGTTCG
AGCTGTCCTGGCTCAATCAGCGCGGCAAGCCGCAGGTGGCGCTGGCCCATATCACCGTGCCCTGCGAGACGCCCCGGATC
GTGGAGAGCAAGTCCTTCAAGCTCTACCTGAACAGCTTTTCCAACACCCGCTTTGCCGATGCCGCGCAGGTGCAGGCGCG
CATCCGTGCCGATGTCAGTGCCGCCGTCTGGCGCGGGGCGGCCAGCGCGGGCAGCGTCGGCGTGACGCTGCTGGGCCCGG
ACCGGTTCGCGCAGGAGCTGATGCAAGAGCTCGATGGCCTGAGCCTGGACCGGCTGGACGTGCAATGCACGCAGTACCAG
CCCGCGCCCGAACTGCTGAGCGCACAGCACGACGCCGCCCCCGTGTCCGAGCGGCTGACCAGCCAACTGCTCAAGAGCAA
CTGCCCGGTCACGGGCCAGCCCGACTGGGCCAGCGTGCAGATCGCCTACCGCGGCCCGCCGATCGACCAGGAAGGGCTGC
TGCAGTATCTGGTGAGCTTTCGCAACCACAGCGGATTCCACGAGCAATGCGTCGAGCGCATCTTCATGGACCTGTGGACG
CGCTGCCAGCCCATCGAACTGACCGTCTACGCGCGCTACACGCGCCGTGGCGGCCTGGACATCAACCCGCTGCGCACCAG
CCACCCGCAGGGCCTGCCGCGCAACATGCGCACGGCGCGGCAATGA

Upstream 100 bases:

>100_bases
AACCGAAAGCCGGTGCTGGCGCATGTCCTGCCTGTGCCCATAGCGGCAAAAAAATGAAGCATCCGCAGCCGCGCAACCAC
CACCCATGCAGGGGCAGGCC

Downstream 100 bases:

>100_bases
GACGGACCGGCGCATGGGCCAGGTACATGGGCCAGACGCATGGGCCATCCCCCGGTGAACTAAAATCGCCCCTCCAAGGG
CCGCCGACGCCCAGGCTCCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNPPESSPLGKASACVDQYDATLLFPIARADQRAELGISGAAPFFGADLWTAFELSWLNQRGKPQVALAHITVPCETPRI
VESKSFKLYLNSFSNTRFADAAQVQARIRADVSAAVWRGAASAGSVGVTLLGPDRFAQELMQELDGLSLDRLDVQCTQYQ
PAPELLSAQHDAAPVSERLTSQLLKSNCPVTGQPDWASVQIAYRGPPIDQEGLLQYLVSFRNHSGFHEQCVERIFMDLWT
RCQPIELTVYARYTRRGGLDINPLRTSHPQGLPRNMRTARQ

Sequences:

>Translated_281_residues
MNPPESSPLGKASACVDQYDATLLFPIARADQRAELGISGAAPFFGADLWTAFELSWLNQRGKPQVALAHITVPCETPRI
VESKSFKLYLNSFSNTRFADAAQVQARIRADVSAAVWRGAASAGSVGVTLLGPDRFAQELMQELDGLSLDRLDVQCTQYQ
PAPELLSAQHDAAPVSERLTSQLLKSNCPVTGQPDWASVQIAYRGPPIDQEGLLQYLVSFRNHSGFHEQCVERIFMDLWT
RCQPIELTVYARYTRRGGLDINPLRTSHPQGLPRNMRTARQ
>Mature_281_residues
MNPPESSPLGKASACVDQYDATLLFPIARADQRAELGISGAAPFFGADLWTAFELSWLNQRGKPQVALAHITVPCETPRI
VESKSFKLYLNSFSNTRFADAAQVQARIRADVSAAVWRGAASAGSVGVTLLGPDRFAQELMQELDGLSLDRLDVQCTQYQ
PAPELLSAQHDAAPVSERLTSQLLKSNCPVTGQPDWASVQIAYRGPPIDQEGLLQYLVSFRNHSGFHEQCVERIFMDLWT
RCQPIELTVYARYTRRGGLDINPLRTSHPQGLPRNMRTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=275, Percent_Identity=46.9090909090909, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_VEREI (A1WS75)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999500.1
- ProteinModelPortal:   A1WS75
- SMR:   A1WS75
- STRING:   A1WS75
- GeneID:   4695296
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4790
- NMPDR:   fig|391735.5.peg.4394
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   A1WS75
- ProtClustDB:   PRK11792
- BioCyc:   VEIS391735:VEIS_4790-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31098; Mature: 31098

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPESSPLGKASACVDQYDATLLFPIARADQRAELGISGAAPFFGADLWTAFELSWLNQ
CCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHCC
RGKPQVALAHITVPCETPRIVESKSFKLYLNSFSNTRFADAAQVQARIRADVSAAVWRGA
CCCCCEEEEEEEECCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ASAGSVGVTLLGPDRFAQELMQELDGLSLDRLDVQCTQYQPAPELLSAQHDAAPVSERLT
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCHHHHHHH
SQLLKSNCPVTGQPDWASVQIAYRGPPIDQEGLLQYLVSFRNHSGFHEQCVERIFMDLWT
HHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RCQPIELTVYARYTRRGGLDINPLRTSHPQGLPRNMRTARQ
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MNPPESSPLGKASACVDQYDATLLFPIARADQRAELGISGAAPFFGADLWTAFELSWLNQ
CCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHCC
RGKPQVALAHITVPCETPRIVESKSFKLYLNSFSNTRFADAAQVQARIRADVSAAVWRGA
CCCCCEEEEEEEECCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ASAGSVGVTLLGPDRFAQELMQELDGLSLDRLDVQCTQYQPAPELLSAQHDAAPVSERLT
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCHHHHHHH
SQLLKSNCPVTGQPDWASVQIAYRGPPIDQEGLLQYLVSFRNHSGFHEQCVERIFMDLWT
HHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RCQPIELTVYARYTRRGGLDINPLRTSHPQGLPRNMRTARQ
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA