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The map label for this gene is gsiB [H]

Identifier: 121611500

GI number: 121611500

Start: 5084993

End: 5086546

Strand: Direct

Name: gsiB [H]

Synonym: Veis_4591

Alternate gene names: 121611500

Gene position: 5084993-5086546 (Clockwise)

Preceding gene: 121611499

Following gene: 121611501

Centisome position: 91.35

GC content: 63.0

Gene sequence:

>1554_bases
ATGAAGCAATCTTCTTTCTCCATGCGCTGGGCATCCGCACTGCTGGGCCTGGCCGCGCTCGCCGCCTCGGGCTCGGCACT
GGCTGCCAAAGACGTGGTGCTGGCCATCGGCTACCAGCCGGAAACGCTGGACCCGTACAACACCAACACGACCATCACCA
CGGCCGTGACCAAGACCTTCTACGAAGGCCTGTTCCAGTTCGACAAGGACCTGAAGGTGCAGAACGCGCTCGCCGAGGGC
TACGAGGTCTCCAAGGACGGCCTGGTCTACACCATCGGGCTGCGCAGCGGCGTCAAGTTCCACGACGGCACCGACTTCAA
CGCCGAAGCCGTGAAGGTCACGCTCGACCGCGTGCTGAACCAGGACAACAAGCTGCTGCGCCGCAACCAGTTCAACCGCG
TCGGCAAGGTCGAGGCGCTGAATCCGAGCACCGTGCGCATCACGCTCAAGGAGCCCTTCGGCCCCTTCATCCACTCGCTG
GCCCACGCCTCGGCCGCGATGATCTCGCCGGCCGCGCTCAAGAAGTGGGGCAACAAGGACATCGCCTTCCACCCCGTGGG
CACAGGCCCCTTCGAGTTCGTCGAGTGGAAGCAGACCGACGCGGTCAAGGCCAAGAAGTTCGACGGCTACTGGAAGAAGG
GCTTTCCGAAGATCGACAACCTGCAGTGGAAGCCGGTGCTCGAAAACAACACGCGCGCCGCGATGCTGCAGACCGGCGAA
GCCGACTTTGCCTTCCCCATTCCCTACGAGCAGGCCGAGCTGCTGAAGAAGAGCGACAGGCTCGAAGTGATGGCATCGCC
CTCGATCATCATGCGCTTCCTGGCCTTCAACATGCTGCAAAAGCCGTATGACAACCCGAAGGTGCGCGAAGCCATCGGCT
ACGCCATCAACAAGGAAGCGCTGGCCAAGGTGGCCTTCGGCGGCTATGCGATTCCTGCGCAAGGCGTGCTGCCCCAGGGC
GTGAAGTTCGCCGAAAAGATGGCGCCCATTCCCTACGACGTGAAGAAGGCCAAGGCCCTGCTGGCCGAAGCCGGCTACCC
GAATGGCTTCGAGTCGGTGCTGTGGGGCGCCTACAACAACACGACCAGCCAGAAGACGATCCAGTTCGTGCAGCAGCAGC
TCGCACAGGTGGGCATCAAGCTGCAGGTGCAGGCGCTCGAAGTGGGCCAGCGCACCGAGATGGTCGAAGCCTGGCCCGAT
CCGAAGACGGCCAAGGTGCGCATGTACTACACGGGCTGGTCGTCGTCCACGGGTGAAGCCGACTGGGGTCTGCGCCCGCT
GTTCGCCTCCGAATCGTGGGCGCCGAAGCTCAACAACATGTCTTTCTACAAGAGCGACGTGGTCGACAACGCACTCGCCA
AGGCGCTGATCACGGTGGACGACAAGGAAAAGGCCGCGCTCTACAAGACCGCGCAGGAAGAGATCCGCAAGGACCTGCCG
CGCGTGCCGCTGGTCACCGAGCAGAACCTGTCGGCGCACGCCAAGCGCCTGTCGGGTGTCTACGTCATGCCTGACGCGAA
CATCAACTTCGACGCGATTGCCGTGTCGAACTGA

Upstream 100 bases:

>100_bases
TTCGTGGCACGGCATGCCATCGGCGGCGCTTTCTGATTCCGAACCGTTTTTTCACCGCAGGCCCCCTGCATTTTTTCCAC
GAACCGACTGGAGTTCTTCC

Downstream 100 bases:

>100_bases
TCGCCGGTTCCTCGTTTTCTGATGGATTGTCCGGCCGGCGGCGGCACTGTGCCACCGCCCGCACGCGACATCTGCACCCC
ATGCTGAATTACTTTCTCAA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKQSSFSMRWASALLGLAALAASGSALAAKDVVLAIGYQPETLDPYNTNTTITTAVTKTFYEGLFQFDKDLKVQNALAEG
YEVSKDGLVYTIGLRSGVKFHDGTDFNAEAVKVTLDRVLNQDNKLLRRNQFNRVGKVEALNPSTVRITLKEPFGPFIHSL
AHASAAMISPAALKKWGNKDIAFHPVGTGPFEFVEWKQTDAVKAKKFDGYWKKGFPKIDNLQWKPVLENNTRAAMLQTGE
ADFAFPIPYEQAELLKKSDRLEVMASPSIIMRFLAFNMLQKPYDNPKVREAIGYAINKEALAKVAFGGYAIPAQGVLPQG
VKFAEKMAPIPYDVKKAKALLAEAGYPNGFESVLWGAYNNTTSQKTIQFVQQQLAQVGIKLQVQALEVGQRTEMVEAWPD
PKTAKVRMYYTGWSSSTGEADWGLRPLFASESWAPKLNNMSFYKSDVVDNALAKALITVDDKEKAALYKTAQEEIRKDLP
RVPLVTEQNLSAHAKRLSGVYVMPDANINFDAIAVSN

Sequences:

>Translated_517_residues
MKQSSFSMRWASALLGLAALAASGSALAAKDVVLAIGYQPETLDPYNTNTTITTAVTKTFYEGLFQFDKDLKVQNALAEG
YEVSKDGLVYTIGLRSGVKFHDGTDFNAEAVKVTLDRVLNQDNKLLRRNQFNRVGKVEALNPSTVRITLKEPFGPFIHSL
AHASAAMISPAALKKWGNKDIAFHPVGTGPFEFVEWKQTDAVKAKKFDGYWKKGFPKIDNLQWKPVLENNTRAAMLQTGE
ADFAFPIPYEQAELLKKSDRLEVMASPSIIMRFLAFNMLQKPYDNPKVREAIGYAINKEALAKVAFGGYAIPAQGVLPQG
VKFAEKMAPIPYDVKKAKALLAEAGYPNGFESVLWGAYNNTTSQKTIQFVQQQLAQVGIKLQVQALEVGQRTEMVEAWPD
PKTAKVRMYYTGWSSSTGEADWGLRPLFASESWAPKLNNMSFYKSDVVDNALAKALITVDDKEKAALYKTAQEEIRKDLP
RVPLVTEQNLSAHAKRLSGVYVMPDANINFDAIAVSN
>Mature_517_residues
MKQSSFSMRWASALLGLAALAASGSALAAKDVVLAIGYQPETLDPYNTNTTITTAVTKTFYEGLFQFDKDLKVQNALAEG
YEVSKDGLVYTIGLRSGVKFHDGTDFNAEAVKVTLDRVLNQDNKLLRRNQFNRVGKVEALNPSTVRITLKEPFGPFIHSL
AHASAAMISPAALKKWGNKDIAFHPVGTGPFEFVEWKQTDAVKAKKFDGYWKKGFPKIDNLQWKPVLENNTRAAMLQTGE
ADFAFPIPYEQAELLKKSDRLEVMASPSIIMRFLAFNMLQKPYDNPKVREAIGYAINKEALAKVAFGGYAIPAQGVLPQG
VKFAEKMAPIPYDVKKAKALLAEAGYPNGFESVLWGAYNNTTSQKTIQFVQQQLAQVGIKLQVQALEVGQRTEMVEAWPD
PKTAKVRMYYTGWSSSTGEADWGLRPLFASESWAPKLNNMSFYKSDVVDNALAKALITVDDKEKAALYKTAQEEIRKDLP
RVPLVTEQNLSAHAKRLSGVYVMPDANINFDAIAVSN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=57.3440643863179, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789966, Length=531, Percent_Identity=28.2485875706215, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1787551, Length=543, Percent_Identity=26.3351749539595, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787762, Length=527, Percent_Identity=25.6166982922201, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=497, Percent_Identity=23.5412474849095, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1789887, Length=340, Percent_Identity=27.9411764705882, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789397, Length=511, Percent_Identity=22.7005870841487, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=509, Percent_Identity=20.6286836935167, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57199; Mature: 57199

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSSFSMRWASALLGLAALAASGSALAAKDVVLAIGYQPETLDPYNTNTTITTAVTKTF
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCCCCEEEHHHHHHH
YEGLFQFDKDLKVQNALAEGYEVSKDGLVYTIGLRSGVKFHDGTDFNAEAVKVTLDRVLN
HHHHHHHCCCHHHHHHHHCCCEECCCCEEEEEECCCCCEECCCCCCCCHHHHEEHHHHHC
QDNKLLRRNQFNRVGKVEALNPSTVRITLKEPFGPFIHSLAHASAAMISPAALKKWGNKD
CCHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
IAFHPVGTGPFEFVEWKQTDAVKAKKFDGYWKKGFPKIDNLQWKPVLENNTRAAMLQTGE
EEEEECCCCCHHHEECCCCCCCHHHHCCCHHHCCCCCCCCEEECEEECCCCCEEEEECCC
ADFAFPIPYEQAELLKKSDRLEVMASPSIIMRFLAFNMLQKPYDNPKVREAIGYAINKEA
CCEECCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHH
LAKVAFGGYAIPAQGVLPQGVKFAEKMAPIPYDVKKAKALLAEAGYPNGFESVLWGAYNN
HHHHHCCCEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCC
TTSQKTIQFVQQQLAQVGIKLQVQALEVGQRTEMVEAWPDPKTAKVRMYYTGWSSSTGEA
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
DWGLRPLFASESWAPKLNNMSFYKSDVVDNALAKALITVDDKEKAALYKTAQEEIRKDLP
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCC
RVPLVTEQNLSAHAKRLSGVYVMPDANINFDAIAVSN
CCCCEECCCHHHHHHHHCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MKQSSFSMRWASALLGLAALAASGSALAAKDVVLAIGYQPETLDPYNTNTTITTAVTKTF
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCCCCEEEHHHHHHH
YEGLFQFDKDLKVQNALAEGYEVSKDGLVYTIGLRSGVKFHDGTDFNAEAVKVTLDRVLN
HHHHHHHCCCHHHHHHHHCCCEECCCCEEEEEECCCCCEECCCCCCCCHHHHEEHHHHHC
QDNKLLRRNQFNRVGKVEALNPSTVRITLKEPFGPFIHSLAHASAAMISPAALKKWGNKD
CCHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
IAFHPVGTGPFEFVEWKQTDAVKAKKFDGYWKKGFPKIDNLQWKPVLENNTRAAMLQTGE
EEEEECCCCCHHHEECCCCCCCHHHHCCCHHHCCCCCCCCEEECEEECCCCCEEEEECCC
ADFAFPIPYEQAELLKKSDRLEVMASPSIIMRFLAFNMLQKPYDNPKVREAIGYAINKEA
CCEECCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHH
LAKVAFGGYAIPAQGVLPQGVKFAEKMAPIPYDVKKAKALLAEAGYPNGFESVLWGAYNN
HHHHHCCCEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCC
TTSQKTIQFVQQQLAQVGIKLQVQALEVGQRTEMVEAWPDPKTAKVRMYYTGWSSSTGEA
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
DWGLRPLFASESWAPKLNNMSFYKSDVVDNALAKALITVDDKEKAALYKTAQEEIRKDLP
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCC
RVPLVTEQNLSAHAKRLSGVYVMPDANINFDAIAVSN
CCCCEECCCHHHHHHHHCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA