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The map label for this gene is mraW

Identifier: 121611471

GI number: 121611471

Start: 5050100

End: 5051104

Strand: Direct

Name: mraW

Synonym: Veis_4562

Alternate gene names: 121611471

Gene position: 5050100-5051104 (Clockwise)

Preceding gene: 121611470

Following gene: 121611472

Centisome position: 90.72

GC content: 69.05

Gene sequence:

>1005_bases
GTGTCATCCGCCCCGTGCCACACCACCGTCTTGCGCGAGCCAGCCGTCGATGCGCTGCTGGCGCGCGCCGGGCCTGCGCC
GGCGGGCACCTGGGTGGATGCGACCTTCGGGCGCGGCGGGCACACGCGCCTGATCCTGTGCCGGCTGGGGCCGCAGGGGC
GGCTCGTGGCCTTCGACAAGGACCCCGAGGCCATCGCCGAAGCAATGCGCATCACCGATGCGCGTTTTTCCATCCGGCAC
CAGGGTTTTGGCCAGTTGGGGCAGTTGCCCGCCGGCAGTCTCGCCGGGGTGTTGATGGATCTGGGGGTGAGTTCGCCCCA
GATCGACAGCCCCGAGCGGGGTTTCAGTTTCCGTTTCGACGGCCCGCTGGACATGCGCATGGACACCACGCGCGGCCTCA
GCGCAGCCGATTGGCTCGCCACGGCCGACGCCGGGCAGATTGCGCAGGTGCTACGTGACTACGGTGAAGAACGGTTTGCT
GGCCTCATTGCAAAGGCGATTGTTGCGCGGCGCCAGGCCCGAGGGCCACTGGCGCGCACCGCCGAACTGGCCGATCTCGT
GGCTGGCGCGGTCAAGACCCGCGAGCCAGGCCAGAACCCGGCCACGCGCACCTTTCAGGCTCTTCGGATTTTCATCAACG
CCGAGCTTGAAGAACTGCAACAGGCGTTAGCGGCCAGCCTCCTGGTGCTACAACCCGGTGGCCGGTTGGTGGTGCTCAGC
TTTCACTCGCTGGAAGACCGCATCGTCAAGCAGTTCATCGCCAGGCATTCGCGCCAGCCGTTTGATCGCCGGGTGCCGTT
TGCCGCACCGCAGGCCATGCAACTGCTGGCGCTGGCGCGTGTGCGGCCCGACGCCGCCGAAGTGGCGGCCAACCCGCGCG
CGCGCAGCGCAATCATGCGCGTGGCCGAGCGCACGCAGGCACACGGGGCCGAGCGCTCCGATATGCGCCGGGCCGAACGC
CCCGATGCGCGCCGGGCCGAACATGGCGAGGTCTTGCCGCCATGA

Upstream 100 bases:

>100_bases
TCGAGCTGTGGGACAAGGCCAGCTACGAAGCGCAGGAAGCCCAAGCGATGCAAGGCGCGATGCCTGATGTCTTCAAGGAC
TTCTCCTTTTGAGGCGCGCC

Downstream 100 bases:

>100_bases
CCCGCTTGAACCTGGTGCTGCTGCTGACGGTGCTGGCCAGTGCACTCTATCTGGTGCACATCCAGTACGAGTCGCGCCGG
CTGTTCACCGCGCTCGACCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSSAPCHTTVLREPAVDALLARAGPAPAGTWVDATFGRGGHTRLILCRLGPQGRLVAFDKDPEAIAEAMRITDARFSIRH
QGFGQLGQLPAGSLAGVLMDLGVSSPQIDSPERGFSFRFDGPLDMRMDTTRGLSAADWLATADAGQIAQVLRDYGEERFA
GLIAKAIVARRQARGPLARTAELADLVAGAVKTREPGQNPATRTFQALRIFINAELEELQQALAASLLVLQPGGRLVVLS
FHSLEDRIVKQFIARHSRQPFDRRVPFAAPQAMQLLALARVRPDAAEVAANPRARSAIMRVAERTQAHGAERSDMRRAER
PDARRAEHGEVLPP

Sequences:

>Translated_334_residues
MSSAPCHTTVLREPAVDALLARAGPAPAGTWVDATFGRGGHTRLILCRLGPQGRLVAFDKDPEAIAEAMRITDARFSIRH
QGFGQLGQLPAGSLAGVLMDLGVSSPQIDSPERGFSFRFDGPLDMRMDTTRGLSAADWLATADAGQIAQVLRDYGEERFA
GLIAKAIVARRQARGPLARTAELADLVAGAVKTREPGQNPATRTFQALRIFINAELEELQQALAASLLVLQPGGRLVVLS
FHSLEDRIVKQFIARHSRQPFDRRVPFAAPQAMQLLALARVRPDAAEVAANPRARSAIMRVAERTQAHGAERSDMRRAER
PDARRAEHGEVLPP
>Mature_333_residues
SSAPCHTTVLREPAVDALLARAGPAPAGTWVDATFGRGGHTRLILCRLGPQGRLVAFDKDPEAIAEAMRITDARFSIRHQ
GFGQLGQLPAGSLAGVLMDLGVSSPQIDSPERGFSFRFDGPLDMRMDTTRGLSAADWLATADAGQIAQVLRDYGEERFAG
LIAKAIVARRQARGPLARTAELADLVAGAVKTREPGQNPATRTFQALRIFINAELEELQQALAASLLVLQPGGRLVVLSF
HSLEDRIVKQFIARHSRQPFDRRVPFAAPQAMQLLALARVRPDAAEVAANPRARSAIMRVAERTQAHGAERSDMRRAERP
DARRAEHGEVLPP

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=330, Percent_Identity=40.9090909090909, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=41.9689119170984, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=54.8286604361371, Blast_Score=322, Evalue=2e-89,
Organism=Drosophila melanogaster, GI62472493, Length=343, Percent_Identity=37.0262390670554, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571637, Length=343, Percent_Identity=37.0262390670554, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_VEREI (A1WRK3)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999278.1
- ProteinModelPortal:   A1WRK3
- SMR:   A1WRK3
- STRING:   A1WRK3
- GeneID:   4693854
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4562
- NMPDR:   fig|391735.5.peg.4182
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- PhylomeDB:   A1WRK3
- ProtClustDB:   PRK00050
- BioCyc:   VEIS391735:VEIS_4562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36203; Mature: 36072

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 83-83 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPCHTTVLREPAVDALLARAGPAPAGTWVDATFGRGGHTRLILCRLGPQGRLVAFDK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEECC
DPEAIAEAMRITDARFSIRHQGFGQLGQLPAGSLAGVLMDLGVSSPQIDSPERGFSFRFD
CHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEC
GPLDMRMDTTRGLSAADWLATADAGQIAQVLRDYGEERFAGLIAKAIVARRQARGPLART
CCCCCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AELADLVAGAVKTREPGQNPATRTFQALRIFINAELEELQQALAASLLVLQPGGRLVVLS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
FHSLEDRIVKQFIARHSRQPFDRRVPFAAPQAMQLLALARVRPDAAEVAANPRARSAIMR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
VAERTQAHGAERSDMRRAERPDARRAEHGEVLPP
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSAPCHTTVLREPAVDALLARAGPAPAGTWVDATFGRGGHTRLILCRLGPQGRLVAFDK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEECC
DPEAIAEAMRITDARFSIRHQGFGQLGQLPAGSLAGVLMDLGVSSPQIDSPERGFSFRFD
CHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEC
GPLDMRMDTTRGLSAADWLATADAGQIAQVLRDYGEERFAGLIAKAIVARRQARGPLART
CCCCCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AELADLVAGAVKTREPGQNPATRTFQALRIFINAELEELQQALAASLLVLQPGGRLVVLS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
FHSLEDRIVKQFIARHSRQPFDRRVPFAAPQAMQLLALARVRPDAAEVAANPRARSAIMR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
VAERTQAHGAERSDMRRAERPDARRAEHGEVLPP
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA