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The map label for this gene is degA [H]

Identifier: 121611425

GI number: 121611425

Start: 4995527

End: 4996582

Strand: Direct

Name: degA [H]

Synonym: Veis_4515

Alternate gene names: 121611425

Gene position: 4995527-4996582 (Clockwise)

Preceding gene: 121611424

Following gene: 121611426

Centisome position: 89.74

GC content: 63.07

Gene sequence:

>1056_bases
ATGCAGGCACCACTTGAAGCCATGGTTTTCGATCGATTGAAGTCCGTCACGAGTCAGGACGTTGCCCATGCAGCCGGGGT
TTCCCAGGCTACTGTCTCGCGCGTGTTCGCCAAGCGCGGCAATGTGCAGCCGATAACGGCCGAGCAAGTGCGCCGTGTCG
CTGGCGAACTGGGTTATCGGCCGAACGTCATGGCGCGCGCCATGCGCACCAGCCGTACCGGCACAGTCGGTATCGTGGTG
TCGCGTCTCGGCAATCCGCTGTATCCGCTGATGCTGCAAACGCTGGGTCCGAAGATGGTCGATGCGGGTCTGCGCATGGT
GGTTTGGAGCGTCGACGAGAGCGATGAGACGTGCGCGATCGATGCGATCAAGGAGCGCTCCGTCGATGGCGTGTTGATGA
TGACGGCCACGGCCGAGTCCTCGGCCTTGCGCGAGGTCGTCAAATCCGGCTTGCCGGTGGTGCTGATCAACCGCGTGGTC
GATGGTTTGCCACTGGATCAAGTGGAGTCGGACAACCGTGCCGGAGGGACCTGCGTGGCGCACTACTTCGCGGCGACCGG
CCGGCGGCGCATCGCGTTGATCGGAGGGCCGCAGGCACCCAGCACCATCCGCAACCGTGACCTTGGTTTCCGAGACCAAC
TCGCTGCGCTTGGCGCACCGCTGAAAAAGCAGTTGACGGCTTATGTCGAGAACTTTTCATACGCGGCGGGGTTTGCCGCC
GCCATGCGTCTGCTCGAACTGGCCACACCCCCCGACGCCATCTTTTGTGCCAATGACGTGTTGGCTCTTGGTGCCCGCGA
TGCCGCGCGACGGCGCGGTGTCCGCGTGCCGGAAGACCTCTGGCTGGTCGGTTATGACGACATTGACATGGCGGCCTGGG
ATGCTTTTGACTTGACCACCATTCGCCAACCCATCAATGAAATGTGCCAGATCGCCACCGACTTGCTCGTCGCGCGCATC
GGTGGCTCCACCAAGACCCCGGGCAAGACCGTGGTGCCCAACGACCTGATCATCCGCGGCTCTACGGGGCGCCACCCTTT
CCCCGCTTTGGGCTAA

Upstream 100 bases:

>100_bases
GTTGTCCGGGCTTGGGACCCTCTTGGAAAAGATCGCTTCCATTGGGCGTACTGCACTTGACAAACAAAATGACTACGAAT
ACATTTTGATCTTCAGATAG

Downstream 100 bases:

>100_bases
GGGCTACGCCCGTCGAACTGCATCCATCAAAACCAACGGAGACAACGATGAACATCACGCGCGCCCGGTTTCTGAAATCC
ACCATTGGCGCCAGTCTGGC

Product: periplasmic-binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MQAPLEAMVFDRLKSVTSQDVAHAAGVSQATVSRVFAKRGNVQPITAEQVRRVAGELGYRPNVMARAMRTSRTGTVGIVV
SRLGNPLYPLMLQTLGPKMVDAGLRMVVWSVDESDETCAIDAIKERSVDGVLMMTATAESSALREVVKSGLPVVLINRVV
DGLPLDQVESDNRAGGTCVAHYFAATGRRRIALIGGPQAPSTIRNRDLGFRDQLAALGAPLKKQLTAYVENFSYAAGFAA
AMRLLELATPPDAIFCANDVLALGARDAARRRGVRVPEDLWLVGYDDIDMAAWDAFDLTTIRQPINEMCQIATDLLVARI
GGSTKTPGKTVVPNDLIIRGSTGRHPFPALG

Sequences:

>Translated_351_residues
MQAPLEAMVFDRLKSVTSQDVAHAAGVSQATVSRVFAKRGNVQPITAEQVRRVAGELGYRPNVMARAMRTSRTGTVGIVV
SRLGNPLYPLMLQTLGPKMVDAGLRMVVWSVDESDETCAIDAIKERSVDGVLMMTATAESSALREVVKSGLPVVLINRVV
DGLPLDQVESDNRAGGTCVAHYFAATGRRRIALIGGPQAPSTIRNRDLGFRDQLAALGAPLKKQLTAYVENFSYAAGFAA
AMRLLELATPPDAIFCANDVLALGARDAARRRGVRVPEDLWLVGYDDIDMAAWDAFDLTTIRQPINEMCQIATDLLVARI
GGSTKTPGKTVVPNDLIIRGSTGRHPFPALG
>Mature_351_residues
MQAPLEAMVFDRLKSVTSQDVAHAAGVSQATVSRVFAKRGNVQPITAEQVRRVAGELGYRPNVMARAMRTSRTGTVGIVV
SRLGNPLYPLMLQTLGPKMVDAGLRMVVWSVDESDETCAIDAIKERSVDGVLMMTATAESSALREVVKSGLPVVLINRVV
DGLPLDQVESDNRAGGTCVAHYFAATGRRRIALIGGPQAPSTIRNRDLGFRDQLAALGAPLKKQLTAYVENFSYAAGFAA
AMRLLELATPPDAIFCANDVLALGARDAARRRGVRVPEDLWLVGYDDIDMAAWDAFDLTTIRQPINEMCQIATDLLVARI
GGSTKTPGKTVVPNDLIIRGSTGRHPFPALG

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=31.8181818181818, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=30.421686746988, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=28.5266457680251, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=27.5147928994083, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.791277258567, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=28.1899109792285, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788474, Length=310, Percent_Identity=29.6774193548387, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=28.2312925170068, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787906, Length=337, Percent_Identity=26.1127596439169, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=25.3205128205128, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=24.7678018575851, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786268, Length=332, Percent_Identity=23.7951807228916, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=24.7058823529412, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=22.8125, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37696; Mature: 37696

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPLEAMVFDRLKSVTSQDVAHAAGVSQATVSRVFAKRGNVQPITAEQVRRVAGELGYR
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
PNVMARAMRTSRTGTVGIVVSRLGNPLYPLMLQTLGPKMVDAGLRMVVWSVDESDETCAI
CHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCEEEEEECCCCCCEEHH
DAIKERSVDGVLMMTATAESSALREVVKSGLPVVLINRVVDGLPLDQVESDNRAGGTCVA
HHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCEEEHHHHHCCCCCHHCCCCCCCCCHHHH
HYFAATGRRRIALIGGPQAPSTIRNRDLGFRDQLAALGAPLKKQLTAYVENFSYAAGFAA
HHHHHCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AMRLLELATPPDAIFCANDVLALGARDAARRRGVRVPEDLWLVGYDDIDMAAWDAFDLTT
HHHHHHHCCCCCCEEECCHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCHHHCCHHHHHH
IRQPINEMCQIATDLLVARIGGSTKTPGKTVVPNDLIIRGSTGRHPFPALG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQAPLEAMVFDRLKSVTSQDVAHAAGVSQATVSRVFAKRGNVQPITAEQVRRVAGELGYR
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
PNVMARAMRTSRTGTVGIVVSRLGNPLYPLMLQTLGPKMVDAGLRMVVWSVDESDETCAI
CHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHCCCEEEEEECCCCCCEEHH
DAIKERSVDGVLMMTATAESSALREVVKSGLPVVLINRVVDGLPLDQVESDNRAGGTCVA
HHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCEEEHHHHHCCCCCHHCCCCCCCCCHHHH
HYFAATGRRRIALIGGPQAPSTIRNRDLGFRDQLAALGAPLKKQLTAYVENFSYAAGFAA
HHHHHCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AMRLLELATPPDAIFCANDVLALGARDAARRRGVRVPEDLWLVGYDDIDMAAWDAFDLTT
HHHHHHHCCCCCCEEECCHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCHHHCCHHHHHH
IRQPINEMCQIATDLLVARIGGSTKTPGKTVVPNDLIIRGSTGRHPFPALG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]