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The map label for this gene is ilvB [H]

Identifier: 121611420

GI number: 121611420

Start: 4989418

End: 4991100

Strand: Direct

Name: ilvB [H]

Synonym: Veis_4509

Alternate gene names: 121611420

Gene position: 4989418-4991100 (Clockwise)

Preceding gene: 121611418

Following gene: 121611421

Centisome position: 89.63

GC content: 67.2

Gene sequence:

>1683_bases
ATGACCACCACCAAGAACGCGCTCGTCCGCACGCTGCTCGAATGTGACGCCACCCATGCATTCACCTTGCCGGGCCTGGG
CATCACATGGATGCTCGATGAGTTCCATGGGGTGCGCGACCGCTTGCGTGTCGTGCTCACGCGCAGCGAGCAGATCGCCT
CGGTCATGGCGCAGGTCTATGGCAGGCTGACCGGGCGTCCGGGTGTCTTCATGGGACAGGGGCCCTTTGCGGCCAGCACG
GGCGCCTTCGGTATTTTGGAAGCGCACTTTGCCGGCTCGCCGATGGTGGTGCTGACCGACACCTCCTGCTATGACGGCTT
TGGCATGTACGGCGTCTATCAGACCATGACGGGCGACTACGGCGCTGCGGACGTGCGCACGGTCATGGGCACGATGACCA
AGTACACCGGTTATGCGACCGAACCCCATGAGGCGGTGTACGGCTTGCAGATGGCTTTCAAGCACGCACAACTGCCGCGC
ATGGGCCCGGCCGCGCTGGTGCTGAAGTCGCCCATCATCCGGCGCGAGATGCAGGACGACCCGCGCGTCCATCTCCATCC
TTCAGAGGGCTACCTGCGCCACACCCCGGCACGCCCCGATGCCAATGGCGTCGCCCATCTGGCGCAGATGATCGCAGAGG
CCCGCAACCCGGTCATCCTGGCCGGGCAGGGCGTGCAGACCGATCGGGCGCGCGCCTTGCTGGCACTGGTCGCGCAAAAG
GCCGGTCTGGCGGTGGCCACCAGCTATAACGGCAAGGGGGTGATCGACGAAACGCTGCCGGTCGCGGTGGGCATGCTCGG
AACCTGGGGCAGCCGCAGCGCCAACCGCATGCTTGGCGGCGCTGACCTGGTGGTGGCACTGGGTGCCAGCCTCGGGCCGG
ACTACCTGCGTTTCCGCGACAAGGGTTTGATCGAGCCGGGCCGCCAGCGCATCGCCCATGTCGACATCGATGCGCGCCAT
GCCGGCTGGGTCTACCCGGTCGATCATGCGCTCACGGGTGACGCCGCCGATGTGCTGGAGATGCTGGCCGACAAGTGCCT
GGGCGAGGAAAACAAGCAGGCGCGTCTCGGCAAGATCGCGCAGAACAACCAGGAACACGGCTTCGGCGTCATGCCACCGA
CCGAGGCCGCCAGCGGCACGCTGCACTACGCCGACATGGTGCGCGTGCTCGACCGCGTGCTCACGCCGCAGGACATGCTG
GTGCTCGACGCGGGCAACAGCCGCATCTGGGTCACCAGCATGTTGCGCCTGCGGACCCCCGGCCAATTGGTGGTCCCCGG
TGGCATCGGCGGCATGGGCTGGGGCCTGCCGGCGGCGGCAGCGACCAAGCTCGTCCATCCGGAGCGCAACACCATCTGCG
TGATCGGCGACGGCGGCGCGGCGATGACGCTCAGCACGCTCGCCACCTGCGTACAGGAACACCTGCCGATCACCGTGGTC
GTGGCCAACAACCAGGGCCTGGGCATGGTGCGCGACAACATGAAGGGCCGCCGCATCGCAGTCGATTTCTCGCCGATGGA
CTTTGCGCAGATCGCGCGCGGCATGGGTTGCATCGGCGTTCGCGTCGACAAGGCCGATGCGCTGGAAGACGCCATCGCAG
CCGCCCGCAGCGCCGATCTGCCGACGGTCATCGACCTGGCCATCGACCCTGCGGCCAGCCACATCGCGGCCTCGGACTAC
TGA

Upstream 100 bases:

>100_bases
ATCTATATGCAATCGCTTGCATTCATGATGAAAAATACATTCATTGAATACGATGCAAAGGCTTGTATCGATCGCATCGC
GACCAGATCCAGGGAAACGA

Downstream 100 bases:

>100_bases
GCGTGGCCATGAAGCCCGTCGCCATCGTCACCGGTGGGTCCAGCAACATCGGCTGGGCCTGCGTGCAACGCTTCTGCGCC
GACCACCGGGTGCTGATCGC

Product: thiamine pyrophosphate-binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTTTKNALVRTLLECDATHAFTLPGLGITWMLDEFHGVRDRLRVVLTRSEQIASVMAQVYGRLTGRPGVFMGQGPFAAST
GAFGILEAHFAGSPMVVLTDTSCYDGFGMYGVYQTMTGDYGAADVRTVMGTMTKYTGYATEPHEAVYGLQMAFKHAQLPR
MGPAALVLKSPIIRREMQDDPRVHLHPSEGYLRHTPARPDANGVAHLAQMIAEARNPVILAGQGVQTDRARALLALVAQK
AGLAVATSYNGKGVIDETLPVAVGMLGTWGSRSANRMLGGADLVVALGASLGPDYLRFRDKGLIEPGRQRIAHVDIDARH
AGWVYPVDHALTGDAADVLEMLADKCLGEENKQARLGKIAQNNQEHGFGVMPPTEAASGTLHYADMVRVLDRVLTPQDML
VLDAGNSRIWVTSMLRLRTPGQLVVPGGIGGMGWGLPAAAATKLVHPERNTICVIGDGGAAMTLSTLATCVQEHLPITVV
VANNQGLGMVRDNMKGRRIAVDFSPMDFAQIARGMGCIGVRVDKADALEDAIAAARSADLPTVIDLAIDPAASHIAASDY

Sequences:

>Translated_560_residues
MTTTKNALVRTLLECDATHAFTLPGLGITWMLDEFHGVRDRLRVVLTRSEQIASVMAQVYGRLTGRPGVFMGQGPFAAST
GAFGILEAHFAGSPMVVLTDTSCYDGFGMYGVYQTMTGDYGAADVRTVMGTMTKYTGYATEPHEAVYGLQMAFKHAQLPR
MGPAALVLKSPIIRREMQDDPRVHLHPSEGYLRHTPARPDANGVAHLAQMIAEARNPVILAGQGVQTDRARALLALVAQK
AGLAVATSYNGKGVIDETLPVAVGMLGTWGSRSANRMLGGADLVVALGASLGPDYLRFRDKGLIEPGRQRIAHVDIDARH
AGWVYPVDHALTGDAADVLEMLADKCLGEENKQARLGKIAQNNQEHGFGVMPPTEAASGTLHYADMVRVLDRVLTPQDML
VLDAGNSRIWVTSMLRLRTPGQLVVPGGIGGMGWGLPAAAATKLVHPERNTICVIGDGGAAMTLSTLATCVQEHLPITVV
VANNQGLGMVRDNMKGRRIAVDFSPMDFAQIARGMGCIGVRVDKADALEDAIAAARSADLPTVIDLAIDPAASHIAASDY
>Mature_559_residues
TTTKNALVRTLLECDATHAFTLPGLGITWMLDEFHGVRDRLRVVLTRSEQIASVMAQVYGRLTGRPGVFMGQGPFAASTG
AFGILEAHFAGSPMVVLTDTSCYDGFGMYGVYQTMTGDYGAADVRTVMGTMTKYTGYATEPHEAVYGLQMAFKHAQLPRM
GPAALVLKSPIIRREMQDDPRVHLHPSEGYLRHTPARPDANGVAHLAQMIAEARNPVILAGQGVQTDRARALLALVAQKA
GLAVATSYNGKGVIDETLPVAVGMLGTWGSRSANRMLGGADLVVALGASLGPDYLRFRDKGLIEPGRQRIAHVDIDARHA
GWVYPVDHALTGDAADVLEMLADKCLGEENKQARLGKIAQNNQEHGFGVMPPTEAASGTLHYADMVRVLDRVLTPQDMLV
LDAGNSRIWVTSMLRLRTPGQLVVPGGIGGMGWGLPAAAATKLVHPERNTICVIGDGGAAMTLSTLATCVQEHLPITVVV
ANNQGLGMVRDNMKGRRIAVDFSPMDFAQIARGMGCIGVRVDKADALEDAIAAARSADLPTVIDLAIDPAASHIAASDY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=26.0473588342441, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1790104, Length=488, Percent_Identity=27.0491803278689, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787096, Length=438, Percent_Identity=24.2009132420091, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=24.3772241992883, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788716, Length=577, Percent_Identity=22.0103986135182, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17542570, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=26.4653641207815, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI19922626, Length=543, Percent_Identity=24.1252302025783, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59647; Mature: 59516

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTKNALVRTLLECDATHAFTLPGLGITWMLDEFHGVRDRLRVVLTRSEQIASVMAQVY
CCCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GRLTGRPGVFMGQGPFAASTGAFGILEAHFAGSPMVVLTDTSCYDGFGMYGVYQTMTGDY
HHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCC
GAADVRTVMGTMTKYTGYATEPHEAVYGLQMAFKHAQLPRMGPAALVLKSPIIRREMQDD
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHCCCC
PRVHLHPSEGYLRHTPARPDANGVAHLAQMIAEARNPVILAGQGVQTDRARALLALVAQK
CCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
AGLAVATSYNGKGVIDETLPVAVGMLGTWGSRSANRMLGGADLVVALGASLGPDYLRFRD
CCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCCCHHHHHCC
KGLIEPGRQRIAHVDIDARHAGWVYPVDHALTGDAADVLEMLADKCLGEENKQARLGKIA
CCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
QNNQEHGFGVMPPTEAASGTLHYADMVRVLDRVLTPQDMLVLDAGNSRIWVTSMLRLRTP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHHHCCC
GQLVVPGGIGGMGWGLPAAAATKLVHPERNTICVIGDGGAAMTLSTLATCVQEHLPITVV
CCEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEE
VANNQGLGMVRDNMKGRRIAVDFSPMDFAQIARGMGCIGVRVDKADALEDAIAAARSADL
EECCCCCEEECCCCCCCEEEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
PTVIDLAIDPAASHIAASDY
CEEEEEEECCCHHHHCCCCC
>Mature Secondary Structure 
TTTKNALVRTLLECDATHAFTLPGLGITWMLDEFHGVRDRLRVVLTRSEQIASVMAQVY
CCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GRLTGRPGVFMGQGPFAASTGAFGILEAHFAGSPMVVLTDTSCYDGFGMYGVYQTMTGDY
HHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHCCCC
GAADVRTVMGTMTKYTGYATEPHEAVYGLQMAFKHAQLPRMGPAALVLKSPIIRREMQDD
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHCCCC
PRVHLHPSEGYLRHTPARPDANGVAHLAQMIAEARNPVILAGQGVQTDRARALLALVAQK
CCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
AGLAVATSYNGKGVIDETLPVAVGMLGTWGSRSANRMLGGADLVVALGASLGPDYLRFRD
CCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCCCHHHHHCC
KGLIEPGRQRIAHVDIDARHAGWVYPVDHALTGDAADVLEMLADKCLGEENKQARLGKIA
CCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
QNNQEHGFGVMPPTEAASGTLHYADMVRVLDRVLTPQDMLVLDAGNSRIWVTSMLRLRTP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHHHCCC
GQLVVPGGIGGMGWGLPAAAATKLVHPERNTICVIGDGGAAMTLSTLATCVQEHLPITVV
CCEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEE
VANNQGLGMVRDNMKGRRIAVDFSPMDFAQIARGMGCIGVRVDKADALEDAIAAARSADL
EECCCCCEEECCCCCCCEEEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
PTVIDLAIDPAASHIAASDY
CEEEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9099862 [H]