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The map label for this gene is mcbR [H]

Identifier: 121611044

GI number: 121611044

Start: 4526353

End: 4527087

Strand: Direct

Name: mcbR [H]

Synonym: Veis_4128

Alternate gene names: 121611044

Gene position: 4526353-4527087 (Clockwise)

Preceding gene: 121611043

Following gene: 121611045

Centisome position: 81.31

GC content: 66.8

Gene sequence:

>735_bases
ATGGCCACCGGCACCAGCCCGATCGAACGCAGCAATCTGGCCAGTCAAATCTATGAGCACCTGCGCGAGCAGCTGATGTC
GGCCACCTTCCAGCCGGGGCAGCGCCTGAAGATCCGCGAACTCGCCAAGACCATGGGCACCAGCGAGACGCCGGTGCGCG
AGGCCCTGATCCAGTTGGTGCGCGACCACGCGCTCGAGATGAAGGAAGGCCACTTCATCCGCGTGCGCATGCTCTCGCTG
GCCGAGTACGTGGAACTGCGCGAAATCCGCCTGGCGCTGGAGCCGCTGGCCGCCGAGCGCGCGGTGCCGTTGCTCGACGA
CGCGGCGCTCGGGCAACTGAGCGAAAGCCACCGCCTGCTGATGCACGCGGAAAAGGCCCGGGACTACCGCGCCGCGCTGC
AGCACAACTTCGACTTTCACTTCGGCATTTACCGCCGCTCCGGCATGCCGCAACTGACCGGCCTGCTCGACCGCATCTGG
ATGCAGGTCGGGCCGATGATGAACTTTCTCTACCCGCACGGCCATCCGCACTACGACGGCGTGCACCAGCATCTGAACGT
GCTGCGCGGCCTGCAAGCGCGCGACGTGAAGATGGTGCACGAGGCGATCCGCAACGACCTGATCGAAGGCGGTCGCGGCT
TCGTGCGCGTGCTGCAAGAGATCGACGCCCACCCCGAGCGCGCAGACGAACTGCTCGCGCTGCGCAACCAGCCCCGCCCG
GCAGAAAGCCCCTGA

Upstream 100 bases:

>100_bases
CCGCCTGGCTCAGCCGCTGATCCGCACCCATCCATCCCGCTCGCGCCGTGCCCTACCATGCGAGCGAACGCACCCGTGAA
CCCAAGACACAAGGAGAGCC

Downstream 100 bases:

>100_bases
CGATGGACCACACCAGCGACCCGAACGCCGCCTGTTACCGCATCGACCCGCAGCGCACCGACCTGGCCCGGGAATTTCGC
AGCCATATCCGCGGACCGTA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MATGTSPIERSNLASQIYEHLREQLMSATFQPGQRLKIRELAKTMGTSETPVREALIQLVRDHALEMKEGHFIRVRMLSL
AEYVELREIRLALEPLAAERAVPLLDDAALGQLSESHRLLMHAEKARDYRAALQHNFDFHFGIYRRSGMPQLTGLLDRIW
MQVGPMMNFLYPHGHPHYDGVHQHLNVLRGLQARDVKMVHEAIRNDLIEGGRGFVRVLQEIDAHPERADELLALRNQPRP
AESP

Sequences:

>Translated_244_residues
MATGTSPIERSNLASQIYEHLREQLMSATFQPGQRLKIRELAKTMGTSETPVREALIQLVRDHALEMKEGHFIRVRMLSL
AEYVELREIRLALEPLAAERAVPLLDDAALGQLSESHRLLMHAEKARDYRAALQHNFDFHFGIYRRSGMPQLTGLLDRIW
MQVGPMMNFLYPHGHPHYDGVHQHLNVLRGLQARDVKMVHEAIRNDLIEGGRGFVRVLQEIDAHPERADELLALRNQPRP
AESP
>Mature_243_residues
ATGTSPIERSNLASQIYEHLREQLMSATFQPGQRLKIRELAKTMGTSETPVREALIQLVRDHALEMKEGHFIRVRMLSLA
EYVELREIRLALEPLAAERAVPLLDDAALGQLSESHRLLMHAEKARDYRAALQHNFDFHFGIYRRSGMPQLTGLLDRIWM
QVGPMMNFLYPHGHPHYDGVHQHLNVLRGLQARDVKMVHEAIRNDLIEGGRGFVRVLQEIDAHPERADELLALRNQPRPA
ESP

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=197, Percent_Identity=31.9796954314721, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27951; Mature: 27819

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTSPIERSNLASQIYEHLREQLMSATFQPGQRLKIRELAKTMGTSETPVREALIQLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
RDHALEMKEGHFIRVRMLSLAEYVELREIRLALEPLAAERAVPLLDDAALGQLSESHRLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MHAEKARDYRAALQHNFDFHFGIYRRSGMPQLTGLLDRIWMQVGPMMNFLYPHGHPHYDG
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VHQHLNVLRGLQARDVKMVHEAIRNDLIEGGRGFVRVLQEIDAHPERADELLALRNQPRP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
AESP
CCCC
>Mature Secondary Structure 
ATGTSPIERSNLASQIYEHLREQLMSATFQPGQRLKIRELAKTMGTSETPVREALIQLV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
RDHALEMKEGHFIRVRMLSLAEYVELREIRLALEPLAAERAVPLLDDAALGQLSESHRLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MHAEKARDYRAALQHNFDFHFGIYRRSGMPQLTGLLDRIWMQVGPMMNFLYPHGHPHYDG
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VHQHLNVLRGLQARDVKMVHEAIRNDLIEGGRGFVRVLQEIDAHPERADELLALRNQPRP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
AESP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]