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The map label for this gene is proW [H]

Identifier: 121610976

GI number: 121610976

Start: 4453575

End: 4454447

Strand: Direct

Name: proW [H]

Synonym: Veis_4060

Alternate gene names: 121610976

Gene position: 4453575-4454447 (Clockwise)

Preceding gene: 121610975

Following gene: 121610977

Centisome position: 80.0

GC content: 64.72

Gene sequence:

>873_bases
ATGTCTGAACTGCTCGGCCGTGCCATCGACGCCTTGCGCGAACTGCTGCCGCTCGGGCGCTGGGTCAACGAAGGCGTCAA
GCTGCTGCTCGACCATGGCGCCACCGTCTTCGATTCGGTGGGCGTGATGGTCGAAGCCTTCGCCCAATGGATAGAACAGG
GCATGCTGGCGATTCCGTTCTGGTTGACGATTGCCGTCGTCAGCGTGATCGGATGGCGCCGCCTCGGCGCCGGCTTTGGC
ATGTTCGTGCTGCTGGCGCTGGCGCTGATCGTGGTCACCGGCTTCTGGCCGCAAACCATGGTCACCTTGGCGCTGACCTT
GTCGGCCACGCTGATCAGCCTGTTTTTTGGCATCCCGCTGGGCATTGGCTGCGCCCGCAGCAACCGTGTGAACGCCTGCG
TGCGCCCGGTGCTGGACTTCATGCAGACCATGCCGGCCTTCGTCTATCTGATACCCGCGGCCATGCTGTTTGGCCTGGGC
CGGGTGCCCGGAACGATCGCCACCGTGATCTTCGCCATGCCGCCGGTGGTCAGGCTCACCAGCCTGGGCATCCGCCAGGT
GAGCACCGAGCAGGTCGAAGCCGGCAACGCCTTTGGCTGCACCTCGACCCAACTGCTGTTCAAGGTGCAACTGCCGATCG
CCATGCCGACCATCATGGCCGGTGTCAACCAGACCATCATGATGGCCTTGTCCATGGTCATCATTGCCTCCATGGTCGGC
GCCGGCGGCCTGGGCAACGACGTGCTGGCCAGCATACAGCGGCTGGACATCGGTCTGGGCTTTGAAAGCGGCCTGGCGGT
GGTGCTGCTGGCCATCATCCTGGACCGGCTCACCGAAAGCTTCGGCGTGGCCCGCCGGGGCCGCGACAACTGA

Upstream 100 bases:

>100_bases
GACAGGGACCAGCCGATCGGCGTCTTCGATGCCAGCGACCGCTTGCTCGGCTGCATCTCCGCGCGCAGCCTGCTCGACAG
AATGTCCCGGGAGGCACGCC

Downstream 100 bases:

>100_bases
GCCGGCGCCAACGACCAGGACGCGCGCCCCATGCCCGCACGCATGCCCGTCAAGCCCGGCGACAGCCCGGATGCCCGCGC
CAAGGCCGGCAGCACCCATA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSELLGRAIDALRELLPLGRWVNEGVKLLLDHGATVFDSVGVMVEAFAQWIEQGMLAIPFWLTIAVVSVIGWRRLGAGFG
MFVLLALALIVVTGFWPQTMVTLALTLSATLISLFFGIPLGIGCARSNRVNACVRPVLDFMQTMPAFVYLIPAAMLFGLG
RVPGTIATVIFAMPPVVRLTSLGIRQVSTEQVEAGNAFGCTSTQLLFKVQLPIAMPTIMAGVNQTIMMALSMVIIASMVG
AGGLGNDVLASIQRLDIGLGFESGLAVVLLAIILDRLTESFGVARRGRDN

Sequences:

>Translated_290_residues
MSELLGRAIDALRELLPLGRWVNEGVKLLLDHGATVFDSVGVMVEAFAQWIEQGMLAIPFWLTIAVVSVIGWRRLGAGFG
MFVLLALALIVVTGFWPQTMVTLALTLSATLISLFFGIPLGIGCARSNRVNACVRPVLDFMQTMPAFVYLIPAAMLFGLG
RVPGTIATVIFAMPPVVRLTSLGIRQVSTEQVEAGNAFGCTSTQLLFKVQLPIAMPTIMAGVNQTIMMALSMVIIASMVG
AGGLGNDVLASIQRLDIGLGFESGLAVVLLAIILDRLTESFGVARRGRDN
>Mature_289_residues
SELLGRAIDALRELLPLGRWVNEGVKLLLDHGATVFDSVGVMVEAFAQWIEQGMLAIPFWLTIAVVSVIGWRRLGAGFGM
FVLLALALIVVTGFWPQTMVTLALTLSATLISLFFGIPLGIGCARSNRVNACVRPVLDFMQTMPAFVYLIPAAMLFGLGR
VPGTIATVIFAMPPVVRLTSLGIRQVSTEQVEAGNAFGCTSTQLLFKVQLPIAMPTIMAGVNQTIMMALSMVIIASMVGA
GGLGNDVLASIQRLDIGLGFESGLAVVLLAIILDRLTESFGVARRGRDN

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=278, Percent_Identity=51.7985611510791, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI1788451, Length=193, Percent_Identity=31.0880829015544, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788449, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30950; Mature: 30818

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLGRAIDALRELLPLGRWVNEGVKLLLDHGATVFDSVGVMVEAFAQWIEQGMLAIPF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
WLTIAVVSVIGWRRLGAGFGMFVLLALALIVVTGFWPQTMVTLALTLSATLISLFFGIPL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIGCARSNRVNACVRPVLDFMQTMPAFVYLIPAAMLFGLGRVPGTIATVIFAMPPVVRLT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
SLGIRQVSTEQVEAGNAFGCTSTQLLFKVQLPIAMPTIMAGVNQTIMMALSMVIIASMVG
HHHHHHHHHHHHHCCCCCCCCHHHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHC
AGGLGNDVLASIQRLDIGLGFESGLAVVLLAIILDRLTESFGVARRGRDN
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELLGRAIDALRELLPLGRWVNEGVKLLLDHGATVFDSVGVMVEAFAQWIEQGMLAIPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
WLTIAVVSVIGWRRLGAGFGMFVLLALALIVVTGFWPQTMVTLALTLSATLISLFFGIPL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIGCARSNRVNACVRPVLDFMQTMPAFVYLIPAAMLFGLGRVPGTIATVIFAMPPVVRLT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
SLGIRQVSTEQVEAGNAFGCTSTQLLFKVQLPIAMPTIMAGVNQTIMMALSMVIIASMVG
HHHHHHHHHHHHHCCCCCCCCHHHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHC
AGGLGNDVLASIQRLDIGLGFESGLAVVLLAIILDRLTESFGVARRGRDN
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]