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The map label for this gene is yiaO [H]

Identifier: 121610872

GI number: 121610872

Start: 4339896

End: 4340903

Strand: Direct

Name: yiaO [H]

Synonym: Veis_3954

Alternate gene names: 121610872

Gene position: 4339896-4340903 (Clockwise)

Preceding gene: 121610871

Following gene: 121610873

Centisome position: 77.96

GC content: 63.89

Gene sequence:

>1008_bases
ATGCCATCGACTCGACCATTGCCCCGACCCTCGTCCAGATCATTGCGCCGCTTGGCCCTCGGCCTGGGCCTTGCGTTCGG
CCTGGGCGCCACAGCGGCCGCGCAGACCACGATGCGCATCAACATCTCGACGGCCCAGAACTCCCACCAGGGCGTGGCCA
TCGACACCTTTGCCAAGGAAGTGGAAAAGCGCACCGGCGGCCGCTACAAGGTGCAGACCTTCTACAACGCGGCGCTGGGC
GCCGAACGCGAATCGGTCGAAGCGGTGCAACTGGGCACGCATGAACTGACGTTCAGTTCCTCCGGCCCGATACCCAATTT
CGTGCCCGAGACCAAGATCCTGGATGTCCCCTTCCTGTTTCGCGACAAGGCCCATGCGCGCGCCGTGCTCGACGGCCCCA
TCGGCCAGGAGTTGCTGACCCGGTTCGACGGCAAAGGGTTCAAGGCCCTGGCCTGGGCCGAGAACGGCTTTCGCCACATG
AGCAACAGCAAGCGCGCGGTCAAGGAACCCGGCGACCTCAAGGGCCTGAAGATGCGCACGATGGAAAACCCCGTGCATAT
CGCCGCGTACAAGGGCTTTGGCATCGTCACCACGCCGATGGCGTTCTCCGAGGTGTTCACTGCGCTGCAGCAAGGCACGG
TCGACGGTCAGGAAAACCCGTTGTCGGTGATCATCTCCGCCAAATTCGACCAAGTGCAAAAGCACCTCACGCTCACCGGT
CATGTCTACTCGCCGGCCCTGTTCCTGATGAACAAGGCCCTGTTCGACAAGCTCCCGGCGGCAGACCAGCAGGCGTTCAT
CGACGCCGCCCGGCAAGGCGCCAAGCTCAACCGTGCGCGCGTCGACGAAGACGATGCCAAGGGCGTGGCCGACCTGCGCG
CCAAGGGCATGACCGTGATCGATAACATCGACAAAGCCCGCTTCGTCGCCGCCCTGGCGCCGGTGAACGCGCAATTCGAA
AAGCAGTTCGGCAAGGCCGCGCTCGAGCAGATCCGCAGCGCCCAATGA

Upstream 100 bases:

>100_bases
ACCGCCATAATCTCGGCCCGGCCGGGGTTTGCGCCATCGTGTTTGCATGGCCCAGGCAATGGGGCCCGATTCATCCCGCC
CGAAATTCACAGGAACCGCC

Downstream 100 bases:

>100_bases
CTTGACCGTTGCCCAGGAGCAGCAAACGCCCGCAGCGCAGGCGGGCGGGCATTTCCATGCCGTCGCTGCCTTTGGCCCCG
CAGCCCACCGACCGTCCGAT

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPSTRPLPRPSSRSLRRLALGLGLAFGLGATAAAQTTMRINISTAQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYNAALG
AERESVEAVQLGTHELTFSSSGPIPNFVPETKILDVPFLFRDKAHARAVLDGPIGQELLTRFDGKGFKALAWAENGFRHM
SNSKRAVKEPGDLKGLKMRTMENPVHIAAYKGFGIVTTPMAFSEVFTALQQGTVDGQENPLSVIISAKFDQVQKHLTLTG
HVYSPALFLMNKALFDKLPAADQQAFIDAARQGAKLNRARVDEDDAKGVADLRAKGMTVIDNIDKARFVAALAPVNAQFE
KQFGKAALEQIRSAQ

Sequences:

>Translated_335_residues
MPSTRPLPRPSSRSLRRLALGLGLAFGLGATAAAQTTMRINISTAQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYNAALG
AERESVEAVQLGTHELTFSSSGPIPNFVPETKILDVPFLFRDKAHARAVLDGPIGQELLTRFDGKGFKALAWAENGFRHM
SNSKRAVKEPGDLKGLKMRTMENPVHIAAYKGFGIVTTPMAFSEVFTALQQGTVDGQENPLSVIISAKFDQVQKHLTLTG
HVYSPALFLMNKALFDKLPAADQQAFIDAARQGAKLNRARVDEDDAKGVADLRAKGMTVIDNIDKARFVAALAPVNAQFE
KQFGKAALEQIRSAQ
>Mature_334_residues
PSTRPLPRPSSRSLRRLALGLGLAFGLGATAAAQTTMRINISTAQNSHQGVAIDTFAKEVEKRTGGRYKVQTFYNAALGA
ERESVEAVQLGTHELTFSSSGPIPNFVPETKILDVPFLFRDKAHARAVLDGPIGQELLTRFDGKGFKALAWAENGFRHMS
NSKRAVKEPGDLKGLKMRTMENPVHIAAYKGFGIVTTPMAFSEVFTALQQGTVDGQENPLSVIISAKFDQVQKHLTLTGH
VYSPALFLMNKALFDKLPAADQQAFIDAARQGAKLNRARVDEDDAKGVADLRAKGMTVIDNIDKARFVAALAPVNAQFEK
QFGKAALEQIRSAQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=301, Percent_Identity=41.1960132890365, Blast_Score=230, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36407; Mature: 36276

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRPLPRPSSRSLRRLALGLGLAFGLGATAAAQTTMRINISTAQNSHQGVAIDTFAKE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEEEECCCCCCCEEHHHHHHH
VEKRTGGRYKVQTFYNAALGAERESVEAVQLGTHELTFSSSGPIPNFVPETKILDVPFLF
HHHHCCCCEEEEEHHHHHHCCCHHHHHHEECCCEEEEECCCCCCCCCCCCCEEECCCHHH
RDKAHARAVLDGPIGQELLTRFDGKGFKALAWAENGFRHMSNSKRAVKEPGDLKGLKMRT
CCCCHHHEEECCCCCHHHHHHHCCCCCEEEEEHHHHHHHHCCCCHHHCCCCCCCCEEEEE
MENPVHIAAYKGFGIVTTPMAFSEVFTALQQGTVDGQENPLSVIISAKFDQVQKHLTLTG
CCCCEEEEEECCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHEEEEC
HVYSPALFLMNKALFDKLPAADQQAFIDAARQGAKLNRARVDEDDAKGVADLRAKGMTVI
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCEE
DNIDKARFVAALAPVNAQFEKQFGKAALEQIRSAQ
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSTRPLPRPSSRSLRRLALGLGLAFGLGATAAAQTTMRINISTAQNSHQGVAIDTFAKE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEEEECCCCCCCEEHHHHHHH
VEKRTGGRYKVQTFYNAALGAERESVEAVQLGTHELTFSSSGPIPNFVPETKILDVPFLF
HHHHCCCCEEEEEHHHHHHCCCHHHHHHEECCCEEEEECCCCCCCCCCCCCEEECCCHHH
RDKAHARAVLDGPIGQELLTRFDGKGFKALAWAENGFRHMSNSKRAVKEPGDLKGLKMRT
CCCCHHHEEECCCCCHHHHHHHCCCCCEEEEEHHHHHHHHCCCCHHHCCCCCCCCEEEEE
MENPVHIAAYKGFGIVTTPMAFSEVFTALQQGTVDGQENPLSVIISAKFDQVQKHLTLTG
CCCCEEEEEECCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHEEEEC
HVYSPALFLMNKALFDKLPAADQQAFIDAARQGAKLNRARVDEDDAKGVADLRAKGMTVI
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCEE
DNIDKARFVAALAPVNAQFEKQFGKAALEQIRSAQ
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]