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The map label for this gene is trmD [H]

Identifier: 121610859

GI number: 121610859

Start: 4327166

End: 4327930

Strand: Direct

Name: trmD [H]

Synonym: Veis_3941

Alternate gene names: 121610859

Gene position: 4327166-4327930 (Clockwise)

Preceding gene: 121610858

Following gene: 121610860

Centisome position: 77.73

GC content: 70.98

Gene sequence:

>765_bases
ATGCGCTTTGACATCATCACCCTGCTTCCCGAACTGTTCGCACCGTTGCTGGCCAGTGGCGTGACGCGGCGCGCCTACGC
CAGCGGGCAGGTCGATGTGCGCCTGTGGAACCCGCGCGCGTATGCCGAAGGCCATTACCGCCGGGTCGATGACCGCCCGT
TTGGCGGCGGGCCGGGCATGGTGCTGCTGGCCGAGCCGCTGGCGCGCTGCCTGGCGGCGATCCGCGCCGGGCGCGGCGAA
CCCCGCGCCGGCCAAGCGCCGCTGGTGCTGTTTTCGCCCATTGGCGAGCCGCTCCGGCACCGCCTGGTGCAGCGCTGGTC
GGCCAGCGCCGGCGCCATCTTGCTGTGCGGGCGGTATGAAGGCGTAGACCAGCGCTTCATCGACGCGCATGTCGACTTGC
AGATCAGCCTGGGCGACTTTGTGTTGTCGGGCGGTGAAATTGCGGCCATGGCCTTGCTCGATGCCGTGGCGCGCCTGCAG
CCCGGCGTGCTCAATGACCAGGACAGCCACCAACTCGACAGCTTCCATCCGGCCCTCGACGGCCTGCTCGACTGCCCGCA
CTACACGCGCCCGCAGACCTGGGCCGGGCAGTCGGTGCCGGCCGCACTGCTGTCGGGCAACCATGCGCAGATCGAGCGCT
GGCGGCGCGAGCAGCGCCTGGCCGTCACCGCCCGCCACCGGCCCGAACTGATCGATGCCGCGCGCCAAGCCGCCCGCCTG
AGCGCTGCCGACGAAGCCTTCCTGGCCCAGCTTGGCGGGGGTTGA

Upstream 100 bases:

>100_bases
TTCCTTTCGTCCCGGCCTTCGTGGACCAGGTGGAACTGCCGCAAAAGCGCATCATCGTCGACTGGCAGCCCGACTATTGA
TCGAAGCGCAGCGCGCACCC

Downstream 100 bases:

>100_bases
CCCAAGGGTTTTCACCGAGTGATTTGATCTGCATCAACGGCGTATTATCTACCGCACGGTCGGTCAATGATGTTCGTGAT
CGATCGATGCAGGACTTCCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRFDIITLLPELFAPLLASGVTRRAYASGQVDVRLWNPRAYAEGHYRRVDDRPFGGGPGMVLLAEPLARCLAAIRAGRGE
PRAGQAPLVLFSPIGEPLRHRLVQRWSASAGAILLCGRYEGVDQRFIDAHVDLQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDQDSHQLDSFHPALDGLLDCPHYTRPQTWAGQSVPAALLSGNHAQIERWRREQRLAVTARHRPELIDAARQAARL
SAADEAFLAQLGGG

Sequences:

>Translated_254_residues
MRFDIITLLPELFAPLLASGVTRRAYASGQVDVRLWNPRAYAEGHYRRVDDRPFGGGPGMVLLAEPLARCLAAIRAGRGE
PRAGQAPLVLFSPIGEPLRHRLVQRWSASAGAILLCGRYEGVDQRFIDAHVDLQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDQDSHQLDSFHPALDGLLDCPHYTRPQTWAGQSVPAALLSGNHAQIERWRREQRLAVTARHRPELIDAARQAARL
SAADEAFLAQLGGG
>Mature_254_residues
MRFDIITLLPELFAPLLASGVTRRAYASGQVDVRLWNPRAYAEGHYRRVDDRPFGGGPGMVLLAEPLARCLAAIRAGRGE
PRAGQAPLVLFSPIGEPLRHRLVQRWSASAGAILLCGRYEGVDQRFIDAHVDLQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDQDSHQLDSFHPALDGLLDCPHYTRPQTWAGQSVPAALLSGNHAQIERWRREQRLAVTARHRPELIDAARQAARL
SAADEAFLAQLGGG

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=253, Percent_Identity=43.8735177865613, Blast_Score=200, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27642; Mature: 27642

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDIITLLPELFAPLLASGVTRRAYASGQVDVRLWNPRAYAEGHYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCC
VLLAEPLARCLAAIRAGRGEPRAGQAPLVLFSPIGEPLRHRLVQRWSASAGAILLCGRYE
EEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCC
GVDQRFIDAHVDLQISLGDFVLSGGEIAAMALLDAVARLQPGVLNDQDSHQLDSFHPALD
CCCHHHHHEEEEEEEEHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GLLDCPHYTRPQTWAGQSVPAALLSGNHAQIERWRREQRLAVTARHRPELIDAARQAARL
HHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SAADEAFLAQLGGG
HHHHHHHHHHHCCC
>Mature Secondary Structure
MRFDIITLLPELFAPLLASGVTRRAYASGQVDVRLWNPRAYAEGHYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCC
VLLAEPLARCLAAIRAGRGEPRAGQAPLVLFSPIGEPLRHRLVQRWSASAGAILLCGRYE
EEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCC
GVDQRFIDAHVDLQISLGDFVLSGGEIAAMALLDAVARLQPGVLNDQDSHQLDSFHPALD
CCCHHHHHEEEEEEEEHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GLLDCPHYTRPQTWAGQSVPAALLSGNHAQIERWRREQRLAVTARHRPELIDAARQAARL
HHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SAADEAFLAQLGGG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA