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The map label for this gene is yiaJ [H]

Identifier: 121610551

GI number: 121610551

Start: 4008766

End: 4009626

Strand: Direct

Name: yiaJ [H]

Synonym: Veis_3624

Alternate gene names: 121610551

Gene position: 4008766-4009626 (Clockwise)

Preceding gene: 121610550

Following gene: 121610556

Centisome position: 72.01

GC content: 66.55

Gene sequence:

>861_bases
ATGGCGGCATCCGACCCCTTGCCGCTCATGAAAAAACTGGTTCCCGGTGTCCCCGTCAAACCCAGCGTTCAGGTGCTGGA
GCGCATGTTTGCGCTGATCGATGTGCTGGCCTCGCGCGAGGAGGCCATCTCGCTGAAGGAGATCAGCGCCAAGACCGGCC
TGCATCCGTCGACCGCACACCGCATCCTGAACGACTTGGCGCTGGGCCGCTTCGTGGACCACCCGGAGTCCGGCAGCTAT
CGCCTGGGCATGCGCTTGCTGGAGTTGGGCAATCTGGTCAAGGCCCGGCTGAGCGTGCGCGATGCCGCACTGATCCCCAT
GCGCAACCTGCATCGATCGATCCAGCAGCCGGTCAATCTGAGCCTGCGCCAGGGCGACGAAATCGTGTATGTCGAGCGCG
CCTACAGCGAGCGCTCCGGCATGCAGGTGGTGCGCGCCATCGGAGGGCGGGCGCCGCTGCACCTGACCTCCACCGGCAAG
CTGTTTCTCGCGCTCGACGACCCGCAGCGGGTGCGCGCCTATGCCATGCGCACCGGGCTTGCCGGCCACACGCGCAACAG
CATTACCCAGTTGCCGGCGCTGGAGCGGGAACTGGCAAAAGCGCGGCAGTCCGGCATCGCACGCGACAACGAGGAACTGG
AACTGGGTGTGCGCTGCATGGCCGCAGGGGTGCATGACGACCAGGGCAAGCTGATTGCCGGCCTGTCGATATCCGCGCCC
GCTGACCGACTCGATGAAAGCTGGTTGCCCAAACTGCAGGCCACGGCCAGCGCAATATCACTGGCCTTGGGCTACACGCC
CGGCGCCCAGGCCCGCAGCGAAACGGCAGCCGGCCGGCGCACCGATGTGCGCGCGGAATGA

Upstream 100 bases:

>100_bases
CGGGCGCTGGCACCGGGTGGACATGCCGCTGTGAACCCGCGCGCGCATTTCACAAGCTAAGATGCGGCGGCGTCCGACCC
TTTGCCGCTGATGGCCGCCG

Downstream 100 bases:

>100_bases
GCCCGGCGGCACTTCATCCGCTGGAATTGCGCGCAGGCGCCGTCAATGACGGCATGGATTCGATCCAGTGGCGCACCCGC
TCGGCATCTGCGATCCGGCT

Product: regulatory protein IclR

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAASDPLPLMKKLVPGVPVKPSVQVLERMFALIDVLASREEAISLKEISAKTGLHPSTAHRILNDLALGRFVDHPESGSY
RLGMRLLELGNLVKARLSVRDAALIPMRNLHRSIQQPVNLSLRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGK
LFLALDDPQRVRAYAMRTGLAGHTRNSITQLPALERELAKARQSGIARDNEELELGVRCMAAGVHDDQGKLIAGLSISAP
ADRLDESWLPKLQATASAISLALGYTPGAQARSETAAGRRTDVRAE

Sequences:

>Translated_286_residues
MAASDPLPLMKKLVPGVPVKPSVQVLERMFALIDVLASREEAISLKEISAKTGLHPSTAHRILNDLALGRFVDHPESGSY
RLGMRLLELGNLVKARLSVRDAALIPMRNLHRSIQQPVNLSLRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGK
LFLALDDPQRVRAYAMRTGLAGHTRNSITQLPALERELAKARQSGIARDNEELELGVRCMAAGVHDDQGKLIAGLSISAP
ADRLDESWLPKLQATASAISLALGYTPGAQARSETAAGRRTDVRAE
>Mature_285_residues
AASDPLPLMKKLVPGVPVKPSVQVLERMFALIDVLASREEAISLKEISAKTGLHPSTAHRILNDLALGRFVDHPESGSYR
LGMRLLELGNLVKARLSVRDAALIPMRNLHRSIQQPVNLSLRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKL
FLALDDPQRVRAYAMRTGLAGHTRNSITQLPALERELAKARQSGIARDNEELELGVRCMAAGVHDDQGKLIAGLSISAPA
DRLDESWLPKLQATASAISLALGYTPGAQARSETAAGRRTDVRAE

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=251, Percent_Identity=34.6613545816733, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=33.6, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=32.9317269076305, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082359, Length=272, Percent_Identity=29.7794117647059, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=30.0813008130081, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790752, Length=227, Percent_Identity=27.3127753303965, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31040; Mature: 30909

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASDPLPLMKKLVPGVPVKPSVQVLERMFALIDVLASREEAISLKEISAKTGLHPSTAH
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RILNDLALGRFVDHPESGSYRLGMRLLELGNLVKARLSVRDAALIPMRNLHRSIQQPVNL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
SLRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLALDDPQRVRAYAMRTGL
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCC
AGHTRNSITQLPALERELAKARQSGIARDNEELELGVRCMAAGVHDDQGKLIAGLSISAP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
ADRLDESWLPKLQATASAISLALGYTPGAQARSETAAGRRTDVRAE
HHHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AASDPLPLMKKLVPGVPVKPSVQVLERMFALIDVLASREEAISLKEISAKTGLHPSTAH
CCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RILNDLALGRFVDHPESGSYRLGMRLLELGNLVKARLSVRDAALIPMRNLHRSIQQPVNL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
SLRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLALDDPQRVRAYAMRTGL
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCC
AGHTRNSITQLPALERELAKARQSGIARDNEELELGVRCMAAGVHDDQGKLIAGLSISAP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
ADRLDESWLPKLQATASAISLALGYTPGAQARSETAAGRRTDVRAE
HHHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]