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The map label for this gene is paaF [C]

Identifier: 121610500

GI number: 121610500

Start: 3948339

End: 3949127

Strand: Direct

Name: paaF [C]

Synonym: Veis_3571

Alternate gene names: 121610500

Gene position: 3948339-3949127 (Clockwise)

Preceding gene: 121610499

Following gene: 121610501

Centisome position: 70.93

GC content: 69.07

Gene sequence:

>789_bases
GTGGCCGATGAATGGGTGCAGACCGCCATCGACGGTGCGCTGGCAACCGTCACCCTGAACCGTCCCGACAAGCTCAACAC
GCTGACGCCGGTGATGCTCGACGCGCTCGAGAACGCGGCCCGCCGGCTGGAGGCCGAGCGCGATGTGCGCGTCGTGATCC
TCACCGGTGCCGGCGAGCGGGCGTTTTGCGCCGGCGCCGACATCCATGCCTGGGCCGCACTCCAGCCCCTGGACATGTGG
CGCCGCTGGGTGCGCCGCGGTCATCAGGTGTTCGACCAGTGGGCGCGTCTGCGCCAGCCGGTGATTGCCGCCCTGAACGG
GCACGCCTTCGGTGGCGGATTGGAATTGGCCATCGCCTGCGACCTGCGGATTGCCGACCAGGCTGCCCAATTCGCCCTGC
CCGAGGCCCGCATCGCCACCTGCCCTGGCTGGTCGGGCACGCAACGCCTGGTGCGCCTGATCGGGCCGAGCGCGGCCAAA
TACCTGGCTTTGAGCGGGCAGCGTCTGGACAGCGCTGGCGCCCTGCGCTGCGGCTTGCTGCATGAGGTGACGACGACGGC
AGCGAAAGGGGCCGCCCTGGAGCGTGCGCAGCAGTTGGCGCAGCAGATGTGCGAACAAGCGCCGGTGTCGCTGCAACTGG
CCAAGCAGCTGATCAACGCGGCGGCAGATGAAGATGCCGCCGCTTGCATGGAGGCCATGGCCGGCGCATTGGCCGCTTTC
ACCGACGATGCGAAAGAGGGTGTGTCCAGCTTTCGGGACAAGCGCCCGCCGCACTACACCGGCCGCTGA

Upstream 100 bases:

>100_bases
GAGACGCCCCTGCGGGTTGCCGACGACTTGCGGCCGATGGCTGCGGCCATTTTTCAGCCCCCGCCGATGGGACTGGTGCT
CAAACCTGCCGGGGTGCGCC

Downstream 100 bases:

>100_bases
CCAGCGGCCACTGATTCCATGACCCCCCCATACGACCCGCGTACTGCGATGAACCCGCAAACGAATCCACCGCCCTACCA
GGGCCGGCTGCTCATCGATG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MADEWVQTAIDGALATVTLNRPDKLNTLTPVMLDALENAARRLEAERDVRVVILTGAGERAFCAGADIHAWAALQPLDMW
RRWVRRGHQVFDQWARLRQPVIAALNGHAFGGGLELAIACDLRIADQAAQFALPEARIATCPGWSGTQRLVRLIGPSAAK
YLALSGQRLDSAGALRCGLLHEVTTTAAKGAALERAQQLAQQMCEQAPVSLQLAKQLINAAADEDAAACMEAMAGALAAF
TDDAKEGVSSFRDKRPPHYTGR

Sequences:

>Translated_262_residues
MADEWVQTAIDGALATVTLNRPDKLNTLTPVMLDALENAARRLEAERDVRVVILTGAGERAFCAGADIHAWAALQPLDMW
RRWVRRGHQVFDQWARLRQPVIAALNGHAFGGGLELAIACDLRIADQAAQFALPEARIATCPGWSGTQRLVRLIGPSAAK
YLALSGQRLDSAGALRCGLLHEVTTTAAKGAALERAQQLAQQMCEQAPVSLQLAKQLINAAADEDAAACMEAMAGALAAF
TDDAKEGVSSFRDKRPPHYTGR
>Mature_261_residues
ADEWVQTAIDGALATVTLNRPDKLNTLTPVMLDALENAARRLEAERDVRVVILTGAGERAFCAGADIHAWAALQPLDMWR
RWVRRGHQVFDQWARLRQPVIAALNGHAFGGGLELAIACDLRIADQAAQFALPEARIATCPGWSGTQRLVRLIGPSAAKY
LALSGQRLDSAGALRCGLLHEVTTTAAKGAALERAQQLAQQMCEQAPVSLQLAKQLINAAADEDAAACMEAMAGALAAFT
DDAKEGVSSFRDKRPPHYTGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=35.0597609561753, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=36.2549800796813, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI68989263, Length=219, Percent_Identity=34.2465753424658, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI20127408, Length=278, Percent_Identity=26.2589928057554, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=31.7647058823529, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI213417737, Length=198, Percent_Identity=28.2828282828283, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI157694516, Length=198, Percent_Identity=28.2828282828283, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI70995211, Length=229, Percent_Identity=30.1310043668122, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=30.8108108108108, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=30.8108108108108, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694520, Length=178, Percent_Identity=28.6516853932584, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI261878539, Length=118, Percent_Identity=39.8305084745763, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=25.4901960784314, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=36.6533864541833, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=31.3953488372093, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=33.2075471698113, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=34.375, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788597, Length=273, Percent_Identity=27.8388278388278, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=30.0546448087432, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=34.0807174887892, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=35.5731225296443, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=31.9066147859922, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=27.027027027027, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=213, Percent_Identity=27.2300469483568, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=27.3809523809524, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=28.6384976525822, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=213, Percent_Identity=28.6384976525822, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=28.6384976525822, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=23.7154150197628, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=28.9099526066351, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=175, Percent_Identity=31.4285714285714, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=175, Percent_Identity=31.4285714285714, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=26.6393442622951, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=201, Percent_Identity=26.865671641791, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=32.5757575757576, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI20129971, Length=268, Percent_Identity=31.7164179104478, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653477, Length=268, Percent_Identity=31.7164179104478, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28571730, Length=241, Percent_Identity=26.9709543568465, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571729, Length=241, Percent_Identity=26.9709543568465, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583077, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583079, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921000, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357171, Length=193, Percent_Identity=30.5699481865285, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=24.3027888446215, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28159; Mature: 28028

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEWVQTAIDGALATVTLNRPDKLNTLTPVMLDALENAARRLEAERDVRVVILTGAGER
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AFCAGADIHAWAALQPLDMWRRWVRRGHQVFDQWARLRQPVIAALNGHAFGGGLELAIAC
CEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DLRIADQAAQFALPEARIATCPGWSGTQRLVRLIGPSAAKYLALSGQRLDSAGALRCGLL
CCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHH
HEVTTTAAKGAALERAQQLAQQMCEQAPVSLQLAKQLINAAADEDAAACMEAMAGALAAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TDDAKEGVSSFRDKRPPHYTGR
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADEWVQTAIDGALATVTLNRPDKLNTLTPVMLDALENAARRLEAERDVRVVILTGAGER
CHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AFCAGADIHAWAALQPLDMWRRWVRRGHQVFDQWARLRQPVIAALNGHAFGGGLELAIAC
CEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DLRIADQAAQFALPEARIATCPGWSGTQRLVRLIGPSAAKYLALSGQRLDSAGALRCGLL
CCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHH
HEVTTTAAKGAALERAQQLAQQMCEQAPVSLQLAKQLINAAADEDAAACMEAMAGALAAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TDDAKEGVSSFRDKRPPHYTGR
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA