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The map label for this gene is pemK [H]

Identifier: 121609813

GI number: 121609813

Start: 3211646

End: 3212008

Strand: Direct

Name: pemK [H]

Synonym: Veis_2866

Alternate gene names: 121609813

Gene position: 3211646-3212008 (Clockwise)

Preceding gene: 121609812

Following gene: 121609825

Centisome position: 57.69

GC content: 60.06

Gene sequence:

>363_bases
ATGACATCGCGCGGCCGTGGCCGCCAGGTCAGGCAAGGCGATATCCTGCTCGTCACGCCCGATCCGGCGGCAGGAAGCGA
AATCCGGGGGGAGCGGCCAGTGCTCGTTCTGTCGAACTCGGAGTTCAATCGCGTTGGCCGGGCACTGGTGGCTGCGATAA
CGCAGGGTGGAAATTTCGACCGGGTCAGAGGCTGGGCTGTATCACTGATGGGCACAGGCACTGAAACACAAGGCGCAGTC
GTCCTGAGCCAATGCCGCATGATCGACTATATCGAGCGCGGTGCCAGGCATGTTGAATCTGTGCCTTCTCATGTCATTGA
AGAAGCATTGGCAAAGCTCCAGGCAGCGCTCGACCCTGAATGA

Upstream 100 bases:

>100_bases
ACACGGCGGCGCAATTGAATGCACAGTGCGATTTGAAGGCCGCAATGCCTTCTGATCTCGTGGCTTGGGAGCGTGCCCCG
GCGGTCGGCAGCGAAGCGCC

Downstream 100 bases:

>100_bases
GTGGTGCGCATTGCAGCCTGAACAAGCCTTTGCCCGATTTTTGGCGCCAAACGAATGTAGTGCGATGCGGGCCTTTTTTC
TCGCGTTTGGTTTGTAGTCG

Product: transcriptional modulator of MazE/toxin, MazF

Products: NA

Alternate protein names: Endoribonuclease PemK; Kid toxin protein [H]

Number of amino acids: Translated: 120; Mature: 119

Protein sequence:

>120_residues
MTSRGRGRQVRQGDILLVTPDPAAGSEIRGERPVLVLSNSEFNRVGRALVAAITQGGNFDRVRGWAVSLMGTGTETQGAV
VLSQCRMIDYIERGARHVESVPSHVIEEALAKLQAALDPE

Sequences:

>Translated_120_residues
MTSRGRGRQVRQGDILLVTPDPAAGSEIRGERPVLVLSNSEFNRVGRALVAAITQGGNFDRVRGWAVSLMGTGTETQGAV
VLSQCRMIDYIERGARHVESVPSHVIEEALAKLQAALDPE
>Mature_119_residues
TSRGRGRQVRQGDILLVTPDPAAGSEIRGERPVLVLSNSEFNRVGRALVAAITQGGNFDRVRGWAVSLMGTGTETQGAVV
LSQCRMIDYIERGARHVESVPSHVIEEALAKLQAALDPE

Specific function: Part of a toxin-antitoxin (TA) module. Probably functions as an mRNA interferase. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Both pemI and PemK proteins bind to t

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1790672, Length=114, Percent_Identity=43.859649122807, Blast_Score=89, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 12923; Mature: 12792

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRGRGRQVRQGDILLVTPDPAAGSEIRGERPVLVLSNSEFNRVGRALVAAITQGGNFD
CCCCCCCCEECCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
RVRGWAVSLMGTGTETQGAVVLSQCRMIDYIERGARHVESVPSHVIEEALAKLQAALDPE
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSRGRGRQVRQGDILLVTPDPAAGSEIRGERPVLVLSNSEFNRVGRALVAAITQGGNFD
CCCCCCCEECCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
RVRGWAVSLMGTGTETQGAVVLSQCRMIDYIERGARHVESVPSHVIEEALAKLQAALDPE
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570 [H]