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The map label for this gene is rho [H]

Identifier: 121609560

GI number: 121609560

Start: 2946310

End: 2947572

Strand: Direct

Name: rho [H]

Synonym: Veis_2604

Alternate gene names: 121609560

Gene position: 2946310-2947572 (Clockwise)

Preceding gene: 121609558

Following gene: 121609569

Centisome position: 52.93

GC content: 61.52

Gene sequence:

>1263_bases
ATGCACTTAAACGAACTCAAGGCACTGCATGTGTCTGAAGTCCTCAAGCAAGCTGAAGACCTCGAAATCGAAAACACCGG
CCGCATGCGCAAGCAGGAGCTGATGTTTGCCATCATCAAAAAGCGCGCCAAAACGGGCGAGCAGGTGTTTGCCGACGGCG
TTCTGGAAATCCTGCCTGACGGATTCGGCTTTCTGCGCAGCCCCGACACCAGTTTCACCGCCAGCACCGATGACATCTAC
ATCTCGCCCAGCCAGGTGCGCCGCTTCAACCTGCACACGGGCGACATGATCGAAGGCGAGGTGCGCACGCCCAAGGATGG
CGAGCGCTACTTTGCCCTGACCAAGCTCGACGCCGTCAACGGCGGCCCGCCCGAGCAGAACAAGCACAAGGTGATGTTCG
AGAACCTGACGCCGCTGTTCCCCAAGGAGCAGATGCGGCTGGAGCGCGAAATCAAGGGCGAGGAGAACATCACCGGCCGC
ATCATCGACATCATTGCGCCGCTGGGTCGCGGCCAGCGCGCGCTGATCGTGGCCCCGCCCAAGAGCGGCAAGACGGTGAT
GATGCAGCACATCGCGCACGCGATCACGGCCAACAACCCCGATGTGCACCTGATGGTGCTGCTGGTCGATGAGCGCCCGG
AAGAAGTGACCGAGATGCAGCGCACGGTCAAGGGCGAGATCATCGCCTCGACCTTCGACGAGCCCGCTGCGCGCCATGTG
CATGTGGCCGAAATGGTGATCGAGCGCGCCAAGCGCCTGGTCGAGCTCAAGAAAGACGTGGTGATCCTGCTGGACTCCAT
CACCCGCCTGGCCCGCGCCTACAACAACGTGGTGCCCTCGTCGGGCAAGGTGCTGTCCGGCGGGGTCGATGCCGCCGCCC
TGCAACGCCCGAAGCGCTTTTTCGGCGCCGCGCGCAAGGTCGAAGAAGGCGGCTCGCTGACCATCATCGCCACGGCGCTG
GTCGACACCGGCAGCCGCATGGACGAAGTGATCTTTGAAGAATTCAAGGGCACCGGCAACTGCGAAATCCACCTGAACCG
CCGCCTGTACGAAAAGCGCGTATTCCCCGCGATCGAACTGAACAAGAGCGGCACGCGGCGCGAAGAACTGCTGCTGGCGC
CCGAGATCCTGCAAAAGACCCGCATCCTGCGCCAGTTCATGTACAGCATGGATGAAATCGAGTCCATGGAACTGATGATC
AAGAACATGAAGGCCACCAAGACCAATGTGGACTTCTTCGACATGATGCGCCGGGGCGGGTGA

Upstream 100 bases:

>100_bases
GGGATCCCCTCAGGGCCGGATCTGCAAGCAGCAGTTCATCCGCCCCCGGATCACCATCCCCCGCCCCAAGATCCCACCCG
CTGCGCCAAAGAGCCCATCC

Downstream 100 bases:

>100_bases
AGCCCGTCGCGGTCGAGCCGGTCATTCACCGGGCGGCATGGCCAGCCGCCGCTCACTTTCGAACACCCGCCGCTCACCGC
TGAGCGGATCGACGAACTCC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAKTGEQVFADGVLEILPDGFGFLRSPDTSFTASTDDIY
ISPSQVRRFNLHTGDMIEGEVRTPKDGERYFALTKLDAVNGGPPEQNKHKVMFENLTPLFPKEQMRLEREIKGEENITGR
IIDIIAPLGRGQRALIVAPPKSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTEMQRTVKGEIIASTFDEPAARHV
HVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLSGGVDAAALQRPKRFFGAARKVEEGGSLTIIATAL
VDTGSRMDEVIFEEFKGTGNCEIHLNRRLYEKRVFPAIELNKSGTRREELLLAPEILQKTRILRQFMYSMDEIESMELMI
KNMKATKTNVDFFDMMRRGG

Sequences:

>Translated_420_residues
MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAKTGEQVFADGVLEILPDGFGFLRSPDTSFTASTDDIY
ISPSQVRRFNLHTGDMIEGEVRTPKDGERYFALTKLDAVNGGPPEQNKHKVMFENLTPLFPKEQMRLEREIKGEENITGR
IIDIIAPLGRGQRALIVAPPKSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTEMQRTVKGEIIASTFDEPAARHV
HVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLSGGVDAAALQRPKRFFGAARKVEEGGSLTIIATAL
VDTGSRMDEVIFEEFKGTGNCEIHLNRRLYEKRVFPAIELNKSGTRREELLLAPEILQKTRILRQFMYSMDEIESMELMI
KNMKATKTNVDFFDMMRRGG
>Mature_420_residues
MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAKTGEQVFADGVLEILPDGFGFLRSPDTSFTASTDDIY
ISPSQVRRFNLHTGDMIEGEVRTPKDGERYFALTKLDAVNGGPPEQNKHKVMFENLTPLFPKEQMRLEREIKGEENITGR
IIDIIAPLGRGQRALIVAPPKSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTEMQRTVKGEIIASTFDEPAARHV
HVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLSGGVDAAALQRPKRFFGAARKVEEGGSLTIIATAL
VDTGSRMDEVIFEEFKGTGNCEIHLNRRLYEKRVFPAIELNKSGTRREELLLAPEILQKTRILRQFMYSMDEIESMELMI
KNMKATKTNVDFFDMMRRGG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=66.2679425837321, Blast_Score=583, Evalue=1e-168,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47393; Mature: 47393

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAKTGEQVFADGVLEILPD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GFGFLRSPDTSFTASTDDIYISPSQVRRFNLHTGDMIEGEVRTPKDGERYFALTKLDAVN
CCCCCCCCCCCCCCCCCCEEECHHHHEEEECCCCCEECCCCCCCCCCCEEEEEEEEECCC
GGPPEQNKHKVMFENLTPLFPKEQMRLEREIKGEENITGRIIDIIAPLGRGQRALIVAPP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEHHCCCCCCEEEEEECC
KSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTEMQRTVKGEIIASTFDEPAARHV
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCHHEECCCCCHHHHHH
HVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLSGGVDAAALQRPKRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
FGAARKVEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNCEIHLNRRLYEKRVFPAIEL
HHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCEEEE
NKSGTRREELLLAPEILQKTRILRQFMYSMDEIESMELMIKNMKATKTNVDFFDMMRRGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAKTGEQVFADGVLEILPD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GFGFLRSPDTSFTASTDDIYISPSQVRRFNLHTGDMIEGEVRTPKDGERYFALTKLDAVN
CCCCCCCCCCCCCCCCCCEEECHHHHEEEECCCCCEECCCCCCCCCCCEEEEEEEEECCC
GGPPEQNKHKVMFENLTPLFPKEQMRLEREIKGEENITGRIIDIIAPLGRGQRALIVAPP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEHHCCCCCCEEEEEECC
KSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTEMQRTVKGEIIASTFDEPAARHV
CCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCHHEECCCCCHHHHHH
HVAEMVIERAKRLVELKKDVVILLDSITRLARAYNNVVPSSGKVLSGGVDAAALQRPKRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
FGAARKVEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNCEIHLNRRLYEKRVFPAIEL
HHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCEEEE
NKSGTRREELLLAPEILQKTRILRQFMYSMDEIESMELMIKNMKATKTNVDFFDMMRRGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]