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The map label for this gene is nasD [H]

Identifier: 121609549

GI number: 121609549

Start: 2933722

End: 2936199

Strand: Direct

Name: nasD [H]

Synonym: Veis_2593

Alternate gene names: 121609549

Gene position: 2933722-2936199 (Clockwise)

Preceding gene: 121609548

Following gene: 121609550

Centisome position: 52.7

GC content: 66.71

Gene sequence:

>2478_bases
ATGAAAAAGCCTCAACTGGTGCTGGTGGGCAACGGCATGGCCGGTGTGCGCACGCTGGAAGAACTGCTCAGGATCGCCCC
CGATCGGTACGACATCACGGTGTTCGGGGCCGAGCCGCACCCGAACTACAACCGTATCCTGCTGTCGCCGGTATTGGCCG
GCGAGCAGAGCATGGACCAGATCATCCTCAACGACTGGTCCTGGTACGCCGAGCACCACATCACGCTGCACAGCGGCTGC
ACGGTGACCGATGCAGACCGCGCGCGCCGCGTGGTCCGTGCCAGCGACGCGGCGGGCAAGGTCATCAGCGCCCAGTACGA
CCGCCTCATCATCGCCACCGGCTCCACCCCCTTCATGCTGCCGATTCCCGGCACCGACCTCCAGGGCGTGCTGACCTACC
GCGACATGGCCGACACCCGGGCCATGATCGCTGCGGCCGCAAGCTACCGGCACGCCGTCGTCATCGGCGGCGGCCTGCTG
GGCCTGGAGGCCGCCAACGGCCTGATGAAGCGCGGCATGCAGGTCACCGTGGTGCATGTGGCCGACTGGCTGATGGAGCG
CCAGCTCGACGCCGTGGCCGGCAAGATGCTGCAGCAGTCGCTGCAAGAGCGCGGCATGCAGTTTCTGCTGGGCGCGCAGA
CGCAGGCGCTGCTCGGCCATGCCGACGGCAACGGGCCAGGGCAAGGGCAAGGGGCCGCCCCGAGGGTGCGCGCCGTCCGA
TTCAAGGACGGCAGCGAACTGCCCGCCGACCTGGTGGTGATGGCCGTCGGCATCCGCCCCAACACCGCGTTGGCCGAAAG
GATGCGTCTGCATGTTCAGCGCGGCATCGTGGTCAGCGACACGCTGCAAACCACCACCGACGCGCGCATCTACGCGGTGG
GCGAATGCGCCGCCCACCGCGGCATCGCCTACGGGCTGGTGGCCCCGCTGTTCGAGCAGGGCAAGGTGCTGGCCAACCAT
CTGGCCGAATTCGGCATCGGCCGCTACCAAGGCTCGCAGACCTCGACCAAGCTCAAGGTCACCGGCATCGACCTGTTCTC
GGCCGGCAACTTTCACGGGGGCGGGCACAGCGAAGAAATCGTGCTCAGCGACCCCGGCGCCGGCGTGTACAAGAAACTCG
TCATCCAGGACGACAAACTCGTGGGCGCCTGCCTGTATGGCGACACGGTAGACGGCAACTGGTACTTCAAGCTGCTGCGC
GATGGCCGCAGCGTGGACGACATCCGCGACAAGCTGATGTTCGGCGAATCCCAGTTGGGCGACACCGGCCACCAGGGCCA
GCACAAGGCCGCAGCGCTGCAAGACAGCGACGAAGTCTGCGGCTGCAACGGCGTGACCAAGGGCCGCATCTGCAAAGCCA
TCCGGGAGCAGGGCCTGTTCACCCTCGAAGAGGTGCGCAAGCACACCAAGGCCAGCGCCAGTTGCGGCTCGTGCACCGGG
CTGGTCGAGCAGATCCTGATGTTCGCCGCCGGCGGCGACTACAGCGCCACGCCCAAAACCCGCGCCCTGTGCGGCTGCAC
CGGGCATGGCCACCAGGCCGTGCGCGATGCCATCCGCACCCTGAAGCTGCTCAGCATCCAGGCGGTCTTCGAGCACCTGC
AATGGCGCACGCCCAACGGCTGCGCCAGTTGCCGCCCGGCCGTGAACTACTATCTCGTCAGCACCTGGCCCAAGGACGCC
AGGGACGACCCGCAAAGCCGCGCCATCAACGAGCGCAGCCACGCCAACATCCAGAAAGACGGCAGCTACAGCGTCATCCC
GCGCATATGGGGCGGCGAGACCACGGCCGACGAACTGCGCCGCATTGCCGATGTCGCCGACAAATACCGTATCCCGACCA
TCAAGCTCACCGGCGGCCAGCGCATCGACCTGCTGGGCGTGAAGAAGGAAGACCTGGTAGGCGTCTGGAAAGACCTGGCC
ATGCCCAGCGGCCACGCCTACGCCAAGGCCCTGCGCACGGTCAAGACCTGCGTGGGCAGCGAATGGTGCCGCATGGGCGT
GCAAGACAGCACCCGCCTGGGCAAGGAACTCGAACGCGCGATGTGGCGCATGTATGCCCCGCACAAACTCAAATTCGCCG
TCTCGGGCTGCCCGCGCAACTGCGCCGAAGCCGGCATCAAGGACGTGGGCATCATCGGCGTGGACAGCGGCTGGGAGATG
TACATCGCCGGCAACGGCGGCATCAAGACCGAGGTGGCGCATTTCTTCACCAAGCTCAAGACCGCCCAGGAGGTGCTGGA
ATACACCGGCGCGTTCTGCGAGCTCTACCGCCAGGAAGGCTGGTACCAGGAGCGCAGCGTGCACTACGTGGGCCGCGTGG
GCCTGGAGCATGTGAAAAAGCGCATTCTCCAAGACCCGGCCGGGCGCAAGGCGCTGTGGGAACGACTGCAATGTGCGCTG
GCCGATGAGCCCGACCCCTGGGTCGAGTTCGACAAGGCGGCGCTGGACACACGCCAGTTTGCCGCGCTGAACGCCTGA

Upstream 100 bases:

>100_bases
CGACTTTTTGTATACGCGCCAGGCGCATGTGGAGACGATGGTCTGACCGTCTCGGGGGCTGGACGCTTTTTTTGTTTTCG
ATCGAACCGACGGAGCCACG

Downstream 100 bases:

>100_bases
GCCGGCACCCGAAGGACCTTGGCCCATGACCGAGCACATTCCCGATTGGCAAGCCATCTGCCGCATCGACGACATCCCGG
TGCTGGGCGCGCGCCGCGTG

Product: nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MKKPQLVLVGNGMAGVRTLEELLRIAPDRYDITVFGAEPHPNYNRILLSPVLAGEQSMDQIILNDWSWYAEHHITLHSGC
TVTDADRARRVVRASDAAGKVISAQYDRLIIATGSTPFMLPIPGTDLQGVLTYRDMADTRAMIAAAASYRHAVVIGGGLL
GLEAANGLMKRGMQVTVVHVADWLMERQLDAVAGKMLQQSLQERGMQFLLGAQTQALLGHADGNGPGQGQGAAPRVRAVR
FKDGSELPADLVVMAVGIRPNTALAERMRLHVQRGIVVSDTLQTTTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANH
LAEFGIGRYQGSQTSTKLKVTGIDLFSAGNFHGGGHSEEIVLSDPGAGVYKKLVIQDDKLVGACLYGDTVDGNWYFKLLR
DGRSVDDIRDKLMFGESQLGDTGHQGQHKAAALQDSDEVCGCNGVTKGRICKAIREQGLFTLEEVRKHTKASASCGSCTG
LVEQILMFAAGGDYSATPKTRALCGCTGHGHQAVRDAIRTLKLLSIQAVFEHLQWRTPNGCASCRPAVNYYLVSTWPKDA
RDDPQSRAINERSHANIQKDGSYSVIPRIWGGETTADELRRIADVADKYRIPTIKLTGGQRIDLLGVKKEDLVGVWKDLA
MPSGHAYAKALRTVKTCVGSEWCRMGVQDSTRLGKELERAMWRMYAPHKLKFAVSGCPRNCAEAGIKDVGIIGVDSGWEM
YIAGNGGIKTEVAHFFTKLKTAQEVLEYTGAFCELYRQEGWYQERSVHYVGRVGLEHVKKRILQDPAGRKALWERLQCAL
ADEPDPWVEFDKAALDTRQFAALNA

Sequences:

>Translated_825_residues
MKKPQLVLVGNGMAGVRTLEELLRIAPDRYDITVFGAEPHPNYNRILLSPVLAGEQSMDQIILNDWSWYAEHHITLHSGC
TVTDADRARRVVRASDAAGKVISAQYDRLIIATGSTPFMLPIPGTDLQGVLTYRDMADTRAMIAAAASYRHAVVIGGGLL
GLEAANGLMKRGMQVTVVHVADWLMERQLDAVAGKMLQQSLQERGMQFLLGAQTQALLGHADGNGPGQGQGAAPRVRAVR
FKDGSELPADLVVMAVGIRPNTALAERMRLHVQRGIVVSDTLQTTTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANH
LAEFGIGRYQGSQTSTKLKVTGIDLFSAGNFHGGGHSEEIVLSDPGAGVYKKLVIQDDKLVGACLYGDTVDGNWYFKLLR
DGRSVDDIRDKLMFGESQLGDTGHQGQHKAAALQDSDEVCGCNGVTKGRICKAIREQGLFTLEEVRKHTKASASCGSCTG
LVEQILMFAAGGDYSATPKTRALCGCTGHGHQAVRDAIRTLKLLSIQAVFEHLQWRTPNGCASCRPAVNYYLVSTWPKDA
RDDPQSRAINERSHANIQKDGSYSVIPRIWGGETTADELRRIADVADKYRIPTIKLTGGQRIDLLGVKKEDLVGVWKDLA
MPSGHAYAKALRTVKTCVGSEWCRMGVQDSTRLGKELERAMWRMYAPHKLKFAVSGCPRNCAEAGIKDVGIIGVDSGWEM
YIAGNGGIKTEVAHFFTKLKTAQEVLEYTGAFCELYRQEGWYQERSVHYVGRVGLEHVKKRILQDPAGRKALWERLQCAL
ADEPDPWVEFDKAALDTRQFAALNA
>Mature_825_residues
MKKPQLVLVGNGMAGVRTLEELLRIAPDRYDITVFGAEPHPNYNRILLSPVLAGEQSMDQIILNDWSWYAEHHITLHSGC
TVTDADRARRVVRASDAAGKVISAQYDRLIIATGSTPFMLPIPGTDLQGVLTYRDMADTRAMIAAAASYRHAVVIGGGLL
GLEAANGLMKRGMQVTVVHVADWLMERQLDAVAGKMLQQSLQERGMQFLLGAQTQALLGHADGNGPGQGQGAAPRVRAVR
FKDGSELPADLVVMAVGIRPNTALAERMRLHVQRGIVVSDTLQTTTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANH
LAEFGIGRYQGSQTSTKLKVTGIDLFSAGNFHGGGHSEEIVLSDPGAGVYKKLVIQDDKLVGACLYGDTVDGNWYFKLLR
DGRSVDDIRDKLMFGESQLGDTGHQGQHKAAALQDSDEVCGCNGVTKGRICKAIREQGLFTLEEVRKHTKASASCGSCTG
LVEQILMFAAGGDYSATPKTRALCGCTGHGHQAVRDAIRTLKLLSIQAVFEHLQWRTPNGCASCRPAVNYYLVSTWPKDA
RDDPQSRAINERSHANIQKDGSYSVIPRIWGGETTADELRRIADVADKYRIPTIKLTGGQRIDLLGVKKEDLVGVWKDLA
MPSGHAYAKALRTVKTCVGSEWCRMGVQDSTRLGKELERAMWRMYAPHKLKFAVSGCPRNCAEAGIKDVGIIGVDSGWEM
YIAGNGGIKTEVAHFFTKLKTAQEVLEYTGAFCELYRQEGWYQERSVHYVGRVGLEHVKKRILQDPAGRKALWERLQCAL
ADEPDPWVEFDKAALDTRQFAALNA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=293, Percent_Identity=23.5494880546075, Blast_Score=77, Evalue=8e-14,
Organism=Homo sapiens, GI226437568, Length=293, Percent_Identity=23.5494880546075, Blast_Score=77, Evalue=1e-13,
Organism=Homo sapiens, GI21389617, Length=293, Percent_Identity=23.5494880546075, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789765, Length=816, Percent_Identity=35.171568627451, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=30.9148264984227, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788892, Length=302, Percent_Identity=27.8145695364238, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=418, Percent_Identity=25.1196172248804, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281359715, Length=306, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI281359713, Length=306, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639250, Length=306, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18543267, Length=306, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639252, Length=306, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639257, Length=306, Percent_Identity=27.4509803921569, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585130, Length=295, Percent_Identity=23.728813559322, Blast_Score=83, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 90151; Mature: 90151

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPQLVLVGNGMAGVRTLEELLRIAPDRYDITVFGAEPHPNYNRILLSPVLAGEQSMDQ
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHCCCHHHHH
IILNDWSWYAEHHITLHSGCTVTDADRARRVVRASDAAGKVISAQYDRLIIATGSTPFML
HHHCCCCEEEEEEEEEECCCEEECHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEE
PIPGTDLQGVLTYRDMADTRAMIAAAASYRHAVVIGGGLLGLEAANGLMKRGMQVTVVHV
ECCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEEHHH
ADWLMERQLDAVAGKMLQQSLQERGMQFLLGAQTQALLGHADGNGPGQGQGAAPRVRAVR
HHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHEEEECCCCCCCCCCCCCCCEEEEE
FKDGSELPADLVVMAVGIRPNTALAERMRLHVQRGIVVSDTLQTTTDARIYAVGECAAHR
ECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEHHHHHC
GIAYGLVAPLFEQGKVLANHLAEFGIGRYQGSQTSTKLKVTGIDLFSAGNFHGGGHSEEI
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEE
VLSDPGAGVYKKLVIQDDKLVGACLYGDTVDGNWYFKLLRDGRSVDDIRDKLMFGESQLG
EEECCCCCHHHHHEEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
DTGHQGQHKAAALQDSDEVCGCNGVTKGRICKAIREQGLFTLEEVRKHTKASASCGSCTG
CCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHH
LVEQILMFAAGGDYSATPKTRALCGCTGHGHQAVRDAIRTLKLLSIQAVFEHLQWRTPNG
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CASCRPAVNYYLVSTWPKDARDDPQSRAINERSHANIQKDGSYSVIPRIWGGETTADELR
CCCCCCCCEEEEEECCCCCCCCCCHHHHCCCHHCCCCCCCCCEEEEEEECCCCCCHHHHH
RIADVADKYRIPTIKLTGGQRIDLLGVKKEDLVGVWKDLAMPSGHAYAKALRTVKTCVGS
HHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
EWCRMGVQDSTRLGKELERAMWRMYAPHKLKFAVSGCPRNCAEAGIKDVGIIGVDSGWEM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEE
YIAGNGGIKTEVAHFFTKLKTAQEVLEYTGAFCELYRQEGWYQERSVHYVGRVGLEHVKK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
RILQDPAGRKALWERLQCALADEPDPWVEFDKAALDTRQFAALNA
HHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure
MKKPQLVLVGNGMAGVRTLEELLRIAPDRYDITVFGAEPHPNYNRILLSPVLAGEQSMDQ
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHCCCHHHHH
IILNDWSWYAEHHITLHSGCTVTDADRARRVVRASDAAGKVISAQYDRLIIATGSTPFML
HHHCCCCEEEEEEEEEECCCEEECHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEE
PIPGTDLQGVLTYRDMADTRAMIAAAASYRHAVVIGGGLLGLEAANGLMKRGMQVTVVHV
ECCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEEHHH
ADWLMERQLDAVAGKMLQQSLQERGMQFLLGAQTQALLGHADGNGPGQGQGAAPRVRAVR
HHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHEEEECCCCCCCCCCCCCCCEEEEE
FKDGSELPADLVVMAVGIRPNTALAERMRLHVQRGIVVSDTLQTTTDARIYAVGECAAHR
ECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEHHHHHC
GIAYGLVAPLFEQGKVLANHLAEFGIGRYQGSQTSTKLKVTGIDLFSAGNFHGGGHSEEI
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEE
VLSDPGAGVYKKLVIQDDKLVGACLYGDTVDGNWYFKLLRDGRSVDDIRDKLMFGESQLG
EEECCCCCHHHHHEEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
DTGHQGQHKAAALQDSDEVCGCNGVTKGRICKAIREQGLFTLEEVRKHTKASASCGSCTG
CCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHH
LVEQILMFAAGGDYSATPKTRALCGCTGHGHQAVRDAIRTLKLLSIQAVFEHLQWRTPNG
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CASCRPAVNYYLVSTWPKDARDDPQSRAINERSHANIQKDGSYSVIPRIWGGETTADELR
CCCCCCCCEEEEEECCCCCCCCCCHHHHCCCHHCCCCCCCCCEEEEEEECCCCCCHHHHH
RIADVADKYRIPTIKLTGGQRIDLLGVKKEDLVGVWKDLAMPSGHAYAKALRTVKTCVGS
HHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
EWCRMGVQDSTRLGKELERAMWRMYAPHKLKFAVSGCPRNCAEAGIKDVGIIGVDSGWEM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEE
YIAGNGGIKTEVAHFFTKLKTAQEVLEYTGAFCELYRQEGWYQERSVHYVGRVGLEHVKK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
RILQDPAGRKALWERLQCALADEPDPWVEFDKAALDTRQFAALNA
HHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]