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The map label for this gene is def1 [H]

Identifier: 120613319

GI number: 120613319

Start: 5232722

End: 5233231

Strand: Direct

Name: def1 [H]

Synonym: Aave_4688

Alternate gene names: 120613319

Gene position: 5232722-5233231 (Clockwise)

Preceding gene: 120613316

Following gene: 120613320

Centisome position: 97.76

GC content: 67.84

Gene sequence:

>510_bases
ATGGCCCTCCTTCCCATCCTCTGCTATCCCGATCCCCGCCTGCACAAGGTGGCCCAGCCCGTCCAGGTGGTGGACGACCG
CGTGCGCGCGCTGCTGGACGACATGCTCGAGACCATGTACGACGCGCAGGGCATCGGGCTGGCGGCCACGCAGGTGGACG
TCCACGAGCGGATCATCACCATCGACGTGTCCGAGGACCGCGACACGCCCCTGGTGCTGATCAACCCGGAGATCCTCTGG
GCCAGCGAGGACAAGCAGGTCGGCGAGGAAGGCTGCCTGTCCGTGCCCGGCATCTACGACGGGGTGGAGCGCTCCACCGC
CGTGCGCGTGCAGGCCCTCGACGCGCAGGGGCAGTCCCGCGTCATCGAGGCCGAGGGACTGCTGGCCATCTGCATCCAGC
ACGAGATGGACCACCTGCTGGGCAAGGTGTTCGTCGAGTACCTCTCCCCCCTCAAGCGCACGCGCATCAAGACCAAGATG
CTCAAGCAGCAGCGGGGTGCCCGGGCATGA

Upstream 100 bases:

>100_bases
TTCTGGCCCCAAGCCCCGGATCGGGCAATGATTATTGCCCTCGCCGCCAGCGGCGCGGCGTGAAAGTGGCGAAAATAGCG
ACATCTTCGTCCGTTGTCCC

Downstream 100 bases:

>100_bases
TCGACCGCACGGCCACGTTCTGGCGCCGCGCCGCGGCGGCCTGCCTGGCCGTGCTGCTGGCCGCCTGCGCATCGCCCGAC
TTCCTGCGCCCGGGCGCGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MALLPILCYPDPRLHKVAQPVQVVDDRVRALLDDMLETMYDAQGIGLAATQVDVHERIITIDVSEDRDTPLVLINPEILW
ASEDKQVGEEGCLSVPGIYDGVERSTAVRVQALDAQGQSRVIEAEGLLAICIQHEMDHLLGKVFVEYLSPLKRTRIKTKM
LKQQRGARA

Sequences:

>Translated_169_residues
MALLPILCYPDPRLHKVAQPVQVVDDRVRALLDDMLETMYDAQGIGLAATQVDVHERIITIDVSEDRDTPLVLINPEILW
ASEDKQVGEEGCLSVPGIYDGVERSTAVRVQALDAQGQSRVIEAEGLLAICIQHEMDHLLGKVFVEYLSPLKRTRIKTKM
LKQQRGARA
>Mature_168_residues
ALLPILCYPDPRLHKVAQPVQVVDDRVRALLDDMLETMYDAQGIGLAATQVDVHERIITIDVSEDRDTPLVLINPEILWA
SEDKQVGEEGCLSVPGIYDGVERSTAVRVQALDAQGQSRVIEAEGLLAICIQHEMDHLLGKVFVEYLSPLKRTRIKTKML
KQQRGARA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=171, Percent_Identity=29.8245614035088, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=57.0588235294118, Blast_Score=185, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.2101910828025, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=28.6624203821656, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18853; Mature: 18722

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILCYPDPRLHKVAQPVQVVDDRVRALLDDMLETMYDAQGIGLAATQVDVHERIIT
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHEEEE
IDVSEDRDTPLVLINPEILWASEDKQVGEEGCLSVPGIYDGVERSTAVRVQALDAQGQSR
EEECCCCCCCEEEECCEEEEECCCHHCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCE
VIEAEGLLAICIQHEMDHLLGKVFVEYLSPLKRTRIKTKMLKQQRGARA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALLPILCYPDPRLHKVAQPVQVVDDRVRALLDDMLETMYDAQGIGLAATQVDVHERIIT
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHEEEE
IDVSEDRDTPLVLINPEILWASEDKQVGEEGCLSVPGIYDGVERSTAVRVQALDAQGQSR
EEECCCCCCCEEEECCEEEEECCCHHCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCE
VIEAEGLLAICIQHEMDHLLGKVFVEYLSPLKRTRIKTKMLKQQRGARA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]