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The map label for this gene is tgt

Identifier: 120613114

GI number: 120613114

Start: 4979008

End: 4980180

Strand: Direct

Name: tgt

Synonym: Aave_4479

Alternate gene names: 120613114

Gene position: 4979008-4980180 (Clockwise)

Preceding gene: 120613112

Following gene: 120613115

Centisome position: 93.02

GC content: 68.12

Gene sequence:

>1173_bases
ATGCTTCAATTCGAACTTCTCGCGACCGATCCCGCCAGCCACGCGCGGCGCGGCACGCTCACGCTCAACCACGGGGTGGT
GCAGACCCCCATCTTCATGCCGGTGGGCACCTACGGCACCGTCAAGGGCGTGATGCCGCGCAGCCTGCACGAGATGGGCG
CGCAGATCATCCTGAGCAACACCTTCCACCTGTGGATGCGACCGGGCCTGGACGTGATGCAGAGCTTCGGCGGCCTGCAC
GGCTTCGAGCAGTGGAACAAGCCCATCCTCACCGACTCGGGCGGCTTCCAGGTCTGGTCGCTGGGCGCCATGCGCAAGAT
CACCGAGGAGGGGGTGCACTTCGCGAGCCCGGTGAACGGCGACAAGCTCTTCATGTCGCCCGAGGTGAGCATGCAGATCC
AGACGGTGCTCAACTCCGACATCGTCATGCAGCTCGACGAATGCACGCCCTACGAGACCAAGGGCCACAAGACCACGGAG
GCCGAGGCGCGCAAGTCGATGGAGATGAGCCTGCGCTGGGCGCGCCGCAGCCAGGACGAGTTCCACCGCCTGGGCAACCC
CAACGCGCTCTTCGGCATCGTGCAGGGCGGCATGTACAAGAACCTGCGCCAGGAATCGCTGGAGCGGCTGGTGGAGATGG
ATTTCCCGGGCTACGCCGTGGGCGGCGTGAGCGTGGGCGAGCCCAAGGACGAGATGCTGGACATCATGGCCCACACGCCG
CACCGGCTGCCGGCCCACAAGCCGCGCTACCTGATGGGCGTGGGCACGCCGGAAGACCTCGTGCAGGGCGTGGCCGACGG
CGTGGACATGTTCGACTGCGTGATGCCCACGCGCAATGCGCGCAACGGCACCATCTTCACCCGCTTCGGCGACCTGAAGA
TCCGCAACGCACGCCACAAGGCCGACCACCAGCCGCTGGACCCGACCTGCACCTGCCACGCCTGCGCGGGCACCGAGGGC
GTGGCCTGGGCCGACGGCGGCCGCGGGGGGTTCAGCCGCGCGTACCTGCACCACCTCGACCGCTGCGGCGAGATGCTGGG
CCCCATGCTCACCACGGTGCACAACCTGCACTACTACCTGAACCTCATGCGGGAGATCCGCGAGTCGCTCGACGCGGGCC
GCTTCGGCGAGTTCCGCGCCCGCTTCGCTGCCGACCGCGCGCAGGGCGTGTAG

Upstream 100 bases:

>100_bases
CTTTCTTCTGGAGGCCGGCCGGAGGGCCGCGGAGCGCGGCGCCGCTGCGGACCAGTGTAGTGCCGGCGCGCGGGCGGCCG
TCTGGGACAATCGCCCGATC

Downstream 100 bases:

>100_bases
GGACGCGCGCGGGCCGGGAAGTCGGCCGGGGGGATCGGCCACGGCCTACGCCCTGGGACACCTGGGCGGCCTACCATGCG
CCCATGCTGACCGTCCACCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLQFELLATDPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLHEMGAQIILSNTFHLWMRPGLDVMQSFGGLH
GFEQWNKPILTDSGGFQVWSLGAMRKITEEGVHFASPVNGDKLFMSPEVSMQIQTVLNSDIVMQLDECTPYETKGHKTTE
AEARKSMEMSLRWARRSQDEFHRLGNPNALFGIVQGGMYKNLRQESLERLVEMDFPGYAVGGVSVGEPKDEMLDIMAHTP
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTIFTRFGDLKIRNARHKADHQPLDPTCTCHACAGTEG
VAWADGGRGGFSRAYLHHLDRCGEMLGPMLTTVHNLHYYLNLMREIRESLDAGRFGEFRARFAADRAQGV

Sequences:

>Translated_390_residues
MLQFELLATDPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLHEMGAQIILSNTFHLWMRPGLDVMQSFGGLH
GFEQWNKPILTDSGGFQVWSLGAMRKITEEGVHFASPVNGDKLFMSPEVSMQIQTVLNSDIVMQLDECTPYETKGHKTTE
AEARKSMEMSLRWARRSQDEFHRLGNPNALFGIVQGGMYKNLRQESLERLVEMDFPGYAVGGVSVGEPKDEMLDIMAHTP
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTIFTRFGDLKIRNARHKADHQPLDPTCTCHACAGTEG
VAWADGGRGGFSRAYLHHLDRCGEMLGPMLTTVHNLHYYLNLMREIRESLDAGRFGEFRARFAADRAQGV
>Mature_390_residues
MLQFELLATDPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLHEMGAQIILSNTFHLWMRPGLDVMQSFGGLH
GFEQWNKPILTDSGGFQVWSLGAMRKITEEGVHFASPVNGDKLFMSPEVSMQIQTVLNSDIVMQLDECTPYETKGHKTTE
AEARKSMEMSLRWARRSQDEFHRLGNPNALFGIVQGGMYKNLRQESLERLVEMDFPGYAVGGVSVGEPKDEMLDIMAHTP
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTIFTRFGDLKIRNARHKADHQPLDPTCTCHACAGTEG
VAWADGGRGGFSRAYLHHLDRCGEMLGPMLTTVHNLHYYLNLMREIRESLDAGRFGEFRARFAADRAQGV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=377, Percent_Identity=44.0318302387268, Blast_Score=303, Evalue=1e-82,
Organism=Escherichia coli, GI1786607, Length=381, Percent_Identity=57.2178477690289, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17544680, Length=385, Percent_Identity=40.7792207792208, Blast_Score=281, Evalue=3e-76,
Organism=Drosophila melanogaster, GI19920510, Length=383, Percent_Identity=42.0365535248042, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI21355311, Length=233, Percent_Identity=27.4678111587983, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_ACIAC (A1TVN0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_972792.1
- ProteinModelPortal:   A1TVN0
- SMR:   A1TVN0
- STRING:   A1TVN0
- GeneID:   4668000
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_4479
- NMPDR:   fig|397945.5.peg.3856
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   A1TVN0
- ProtClustDB:   PRK00112
- BioCyc:   AAVE397945:AAVE_4479-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43485; Mature: 43485

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQFELLATDPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLHEMGAQIILSN
CEEEEEEECCCHHHCCCCEEEECCCEEECCEEEECCCCCCHHCCCHHHHHHCCCEEEEEC
TFHLWMRPGLDVMQSFGGLHGFEQWNKPILTDSGGFQVWSLGAMRKITEEGVHFASPVNG
CEEEEECCCHHHHHHHCCCCCHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCC
DKLFMSPEVSMQIQTVLNSDIVMQLDECTPYETKGHKTTEAEARKSMEMSLRWARRSQDE
CEEEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
FHRLGNPNALFGIVQGGMYKNLRQESLERLVEMDFPGYAVGGVSVGEPKDEMLDIMAHTP
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCC
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTIFTRFGDLKIRNARHK
CCCCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCEEEECCHHC
ADHQPLDPTCTCHACAGTEGVAWADGGRGGFSRAYLHHLDRCGEMLGPMLTTVHNLHYYL
CCCCCCCCCCEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLMREIRESLDAGRFGEFRARFAADRAQGV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQFELLATDPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLHEMGAQIILSN
CEEEEEEECCCHHHCCCCEEEECCCEEECCEEEECCCCCCHHCCCHHHHHHCCCEEEEEC
TFHLWMRPGLDVMQSFGGLHGFEQWNKPILTDSGGFQVWSLGAMRKITEEGVHFASPVNG
CEEEEECCCHHHHHHHCCCCCHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCEECCCCCC
DKLFMSPEVSMQIQTVLNSDIVMQLDECTPYETKGHKTTEAEARKSMEMSLRWARRSQDE
CEEEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
FHRLGNPNALFGIVQGGMYKNLRQESLERLVEMDFPGYAVGGVSVGEPKDEMLDIMAHTP
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCC
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTIFTRFGDLKIRNARHK
CCCCCCCCCEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCEEEECCHHC
ADHQPLDPTCTCHACAGTEGVAWADGGRGGFSRAYLHHLDRCGEMLGPMLTTVHNLHYYL
CCCCCCCCCCEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLMREIRESLDAGRFGEFRARFAADRAQGV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA