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The map label for this gene is alaXM [H]

Identifier: 120613091

GI number: 120613091

Start: 4952237

End: 4953004

Strand: Direct

Name: alaXM [H]

Synonym: Aave_4455

Alternate gene names: 120613091

Gene position: 4952237-4953004 (Clockwise)

Preceding gene: 120613090

Following gene: 120613092

Centisome position: 92.52

GC content: 72.53

Gene sequence:

>768_bases
ATGACCCACGACGAAGCCCTCTCCGCTCCCACCCGCGACCTGTTCCGTGAAGACGCCTACCTGCGCGAATGCCGTGCCAC
CGTGGCCGCGGTGCTGCAAGGCGGCGGCATCGTGCTCGACCGCACGGTGTTCTATCCGCTGGGCGGCGGGCAGGCGGGCG
ATGCCGGGGTGCTGGTGCGGGCCGACGGGCGCGAGGTGCCGATCACTGATACGCGCAAGGGCAAGGATGCCGAAGGCAAG
CCCACGGCCGCCATCGTGCACGTGCCGGCGCCCGGCGCCGAGCCGCCCGCCGTGGGCGAGGAAGTCACCGCGCGCATCGA
CTGGGAGCGCCGCCACCGGCTGATGCGCTTCCACACCACCACCCACCTGCTGTGCCATCTGGTGGCGCAGCCGGTGAACG
GCTGCTCGGTCACGCCCGACGCCGCGCGGCTGGATTTCCACATGGACGGCCCGCTGGATGCCGCCGCGCTGAACGCCGGC
ATCGCCCGGCTCGTGGCCGAGGCGCACCCGGTGGCGATCGGCGCGATCACCGATGCCGAGCTGGACGCGCAGCCCGAACT
CGTCAAGAGCATGAGCGTGCAGCCGCCGCGCGGCAGCGGCCAGGTGCGCACCGTGCGCGTGGGCACCGACGGCCAGACCC
AGATCGACCTGCAGCCCTGCGGCGGCACGCACGTCGCCAACACCGCCGAGATCGGCGCGGTGGTCGTCACCAAGATCGAG
AAAAAAGGCGCGATGGCCCGGCGCGTGGTGCTCGGCTTCGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCACGGCGTCCGCTGGCATGGCCTGCTCCGCGGCCTTCGGACAGGGCTGGCCGCAGGACGTCCCAGCGGCGTGCAGGAG
GACGGCGCTACCATCGCCGG

Downstream 100 bases:

>100_bases
GCGCCACCGCTGCACGCCGCCCTTCCCGGAACCAAAGCCGCCCGCGCCGCTCCCACACCGCGCCATGTCCGTCCGCCTCC
TGCAGCGCCTGCTGCCGTCC

Product: metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; AlaXp; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTHDEALSAPTRDLFREDAYLRECRATVAAVLQGGGIVLDRTVFYPLGGGQAGDAGVLVRADGREVPITDTRKGKDAEGK
PTAAIVHVPAPGAEPPAVGEEVTARIDWERRHRLMRFHTTTHLLCHLVAQPVNGCSVTPDAARLDFHMDGPLDAAALNAG
IARLVAEAHPVAIGAITDAELDAQPELVKSMSVQPPRGSGQVRTVRVGTDGQTQIDLQPCGGTHVANTAEIGAVVVTKIE
KKGAMARRVVLGFAA

Sequences:

>Translated_255_residues
MTHDEALSAPTRDLFREDAYLRECRATVAAVLQGGGIVLDRTVFYPLGGGQAGDAGVLVRADGREVPITDTRKGKDAEGK
PTAAIVHVPAPGAEPPAVGEEVTARIDWERRHRLMRFHTTTHLLCHLVAQPVNGCSVTPDAARLDFHMDGPLDAAALNAG
IARLVAEAHPVAIGAITDAELDAQPELVKSMSVQPPRGSGQVRTVRVGTDGQTQIDLQPCGGTHVANTAEIGAVVVTKIE
KKGAMARRVVLGFAA
>Mature_254_residues
THDEALSAPTRDLFREDAYLRECRATVAAVLQGGGIVLDRTVFYPLGGGQAGDAGVLVRADGREVPITDTRKGKDAEGKP
TAAIVHVPAPGAEPPAVGEEVTARIDWERRHRLMRFHTTTHLLCHLVAQPVNGCSVTPDAARLDFHMDGPLDAAALNAGI
ARLVAEAHPVAIGAITDAELDAQPELVKSMSVQPPRGSGQVRTVRVGTDGQTQIDLQPCGGTHVANTAEIGAVVVTKIEK
KGAMARRVVLGFAA

Specific function: Functions in trans to edit the amino acid moiety from mischarged Ser-tRNA(Ala). Recognition depends, at least in part, on the acceptor stem of tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=237, Percent_Identity=28.6919831223629, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26832; Mature: 26701

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDEALSAPTRDLFREDAYLRECRATVAAVLQGGGIVLDRTVFYPLGGGQAGDAGVLVR
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEEEEE
ADGREVPITDTRKGKDAEGKPTAAIVHVPAPGAEPPAVGEEVTARIDWERRHRLMRFHTT
ECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHEEEECHHHHHHHHHHHHH
THLLCHLVAQPVNGCSVTPDAARLDFHMDGPLDAAALNAGIARLVAEAHPVAIGAITDAE
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
LDAQPELVKSMSVQPPRGSGQVRTVRVGTDGQTQIDLQPCGGTHVANTAEIGAVVVTKIE
CCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHCEEEEEEEH
KKGAMARRVVLGFAA
HHCHHHHHHEEEECC
>Mature Secondary Structure 
THDEALSAPTRDLFREDAYLRECRATVAAVLQGGGIVLDRTVFYPLGGGQAGDAGVLVR
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEEEEE
ADGREVPITDTRKGKDAEGKPTAAIVHVPAPGAEPPAVGEEVTARIDWERRHRLMRFHTT
ECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHEEEECHHHHHHHHHHHHH
THLLCHLVAQPVNGCSVTPDAARLDFHMDGPLDAAALNAGIARLVAEAHPVAIGAITDAE
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
LDAQPELVKSMSVQPPRGSGQVRTVRVGTDGQTQIDLQPCGGTHVANTAEIGAVVVTKIE
CCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHCEEEEEEEH
KKGAMARRVVLGFAA
HHCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]