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The map label for this gene is flgJ [H]

Identifier: 120613065

GI number: 120613065

Start: 4921953

End: 4922855

Strand: Direct

Name: flgJ [H]

Synonym: Aave_4429

Alternate gene names: 120613065

Gene position: 4921953-4922855 (Clockwise)

Preceding gene: 120613064

Following gene: 120613066

Centisome position: 91.95

GC content: 68.44

Gene sequence:

>903_bases
ATGTCGCTCAGCCTGACCTCCTCCGCCCCCGCCACGGCCTCCAACGCGCTGGCGGTGGACGCGCGCTCGCTGGACGCGCT
GAAGTTCCAGGCCGGAAGCAACGACCCGAAGGCCGCCAAGGAAGCGGCCAAGCAGTTCGAATCGCTGTTCATGCGCGAGA
TGATCAAGAGCATGCGCGAGGCGACGATGAAGTCGGGCCTGATGGAGGGCGAGCAGGGCAACCTGGGCCAGGACATGCTC
GACCAGCAGTTCTCGGTACAGATGTCGGGCCTGCAGGGCGGCCTCTCCGAAGCCATCCAGCGCCAGCTCACGCGCCAGAT
CGGCAGCGGCCAGGAAGCCGCGCCGGCATTCTCGCCCCCCTCCACGCTGAGCATGCAGGCCCCTTCGCCGGCCCGCGCCG
CCGCGGCGGCCAACGCCTACGCACCGGCGCCCAAGGGCCGCGACGGCTTCGTGCAGCACCACCGCGAGGCCGCCGAGCGC
GTGGCGCAGGAAAGCGGCATTCCCGCGAGCTTCATGCTCGGCCAGGCCGGCCACGAAACCGGCTGGGGCAAGAGCGAGAT
CCGCAGCAAGGACGGCAGCAATTCGTTCAACCTGTTCGGCATCAAGGCCGGCAAGGGCTGGACGGGCAAGGTGGCGGAGA
TCACCACCACCGAATACATCAACGGCACGCCGCGCAAGGTGACGGCGAAGTTCCGCGCCTACGATTCCTACGAGGACTCC
TTCAAGGACTACGCGCGCCTCATCAACGACAACCCACGCTACGAGAAGGCGCGCGGCAAGACGCATTCGGCCGTGGCCTA
CGCCGCCGAGCTGCAGAAGGCCGGCTACGCCACCGACCCGGAATACGCGAGCAAGCTGAGCCGGGCCATCCACAGCACGC
TGCGCGTGGGGCCCTCGGCATGA

Upstream 100 bases:

>100_bases
CATCCTGCAGGCCATCAAGGCCGCGGGCGCCCTCAACGCCGAGCTGGAGGTCATCTGATGGCCGCCGCGTCCACCTCCGC
ATGACCGCCGGGAGACCGCC

Downstream 100 bases:

>100_bases
AGGCGGGTGAACGCACATGAGTCTGCTCAACGTCGGCGCGCGCGCCCTTCTCGCCAACCAGGTGGCCCTGCAGACCACCG
GCCACAACATCGCCAACGTC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSLSLTSSAPATASNALAVDARSLDALKFQAGSNDPKAAKEAAKQFESLFMREMIKSMREATMKSGLMEGEQGNLGQDML
DQQFSVQMSGLQGGLSEAIQRQLTRQIGSGQEAAPAFSPPSTLSMQAPSPARAAAAANAYAPAPKGRDGFVQHHREAAER
VAQESGIPASFMLGQAGHETGWGKSEIRSKDGSNSFNLFGIKAGKGWTGKVAEITTTEYINGTPRKVTAKFRAYDSYEDS
FKDYARLINDNPRYEKARGKTHSAVAYAAELQKAGYATDPEYASKLSRAIHSTLRVGPSA

Sequences:

>Translated_300_residues
MSLSLTSSAPATASNALAVDARSLDALKFQAGSNDPKAAKEAAKQFESLFMREMIKSMREATMKSGLMEGEQGNLGQDML
DQQFSVQMSGLQGGLSEAIQRQLTRQIGSGQEAAPAFSPPSTLSMQAPSPARAAAAANAYAPAPKGRDGFVQHHREAAER
VAQESGIPASFMLGQAGHETGWGKSEIRSKDGSNSFNLFGIKAGKGWTGKVAEITTTEYINGTPRKVTAKFRAYDSYEDS
FKDYARLINDNPRYEKARGKTHSAVAYAAELQKAGYATDPEYASKLSRAIHSTLRVGPSA
>Mature_299_residues
SLSLTSSAPATASNALAVDARSLDALKFQAGSNDPKAAKEAAKQFESLFMREMIKSMREATMKSGLMEGEQGNLGQDMLD
QQFSVQMSGLQGGLSEAIQRQLTRQIGSGQEAAPAFSPPSTLSMQAPSPARAAAAANAYAPAPKGRDGFVQHHREAAERV
AQESGIPASFMLGQAGHETGWGKSEIRSKDGSNSFNLFGIKAGKGWTGKVAEITTTEYINGTPRKVTAKFRAYDSYEDSF
KDYARLINDNPRYEKARGKTHSAVAYAAELQKAGYATDPEYASKLSRAIHSTLRVGPSA

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=282, Percent_Identity=40.0709219858156, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 32080; Mature: 31949

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLTSSAPATASNALAVDARSLDALKFQAGSNDPKAAKEAAKQFESLFMREMIKSMRE
CCCCCCCCCCCCCCCCEEECHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATMKSGLMEGEQGNLGQDMLDQQFSVQMSGLQGGLSEAIQRQLTRQIGSGQEAAPAFSPP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
STLSMQAPSPARAAAAANAYAPAPKGRDGFVQHHREAAERVAQESGIPASFMLGQAGHET
CCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
GWGKSEIRSKDGSNSFNLFGIKAGKGWTGKVAEITTTEYINGTPRKVTAKFRAYDSYEDS
CCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCHHHH
FKDYARLINDNPRYEKARGKTHSAVAYAAELQKAGYATDPEYASKLSRAIHSTLRVGPSA
HHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLSLTSSAPATASNALAVDARSLDALKFQAGSNDPKAAKEAAKQFESLFMREMIKSMRE
CCCCCCCCCCCCCCCEEECHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATMKSGLMEGEQGNLGQDMLDQQFSVQMSGLQGGLSEAIQRQLTRQIGSGQEAAPAFSPP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
STLSMQAPSPARAAAAANAYAPAPKGRDGFVQHHREAAERVAQESGIPASFMLGQAGHET
CCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
GWGKSEIRSKDGSNSFNLFGIKAGKGWTGKVAEITTTEYINGTPRKVTAKFRAYDSYEDS
CCCHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCHHHH
FKDYARLINDNPRYEKARGKTHSAVAYAAELQKAGYATDPEYASKLSRAIHSTLRVGPSA
HHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]