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The map label for this gene is rffA [H]

Identifier: 120613042

GI number: 120613042

Start: 4897111

End: 4898265

Strand: Direct

Name: rffA [H]

Synonym: Aave_4406

Alternate gene names: 120613042

Gene position: 4897111-4898265 (Clockwise)

Preceding gene: 120613041

Following gene: 120613043

Centisome position: 91.49

GC content: 61.3

Gene sequence:

>1155_bases
ATGGACAACTCCAGCATCCCTTTCGGCCAGCCGTTCATCGTCGGCAAGGAGCTTTTCTACATCGCCCAGGCAGTCATGAA
CGGCTCCATCGCCGGCGACGGGGATTTCACGCGCAAATGCCAGGAGTGGCTAGAGGTCCATCTGTCGTGCAAGAAGGCCC
TTCTGACCACGTCCTGCACCACGGCGCTCGAAATGGCGGCCATCCTGACAGATGTGCAACCGGGCGACGAAATCATCATG
CCGTCGTTCACGTTCGTCTCGACGGCGAATGCCTTCGTGCTGCGCGGCGGCGTTCCGGTCTTCGTCGATATCCGCGCCGA
CACGCTGAATCTGGATGAGCGTCTGGTGGAGGCGCTCATCACGCCGCGCACCAAGGCGGTCGTGGCCGTGCATTACGCAG
GGTTTCCCTGCGCCATGGACAAGCTCAAGGCCCTGTGCCGCCAGCACGGCCTGCGGCTCATCGAAGACGCCGCACAAGCC
ATCCTGACGCACGACGAAGGCCAAGCGTTGGGCACGATCGGCGACATGGGTTGCCTCAGCTTCCATGAAACCAAGAACGT
CATCTGCGGCGAGGGCGGGGCCCTGCTCATCAACGACCCGGCGCTGATCGAGCGCGCCGAAATCATCTGGCAGAAAGGAA
CCAACCGCAAAGCCTTCTTCAGAGGCGAAGTGGACAGGTATTCCTGGGTCGACATAGGCTCGTCGTTCCTGCCGAATGAA
CTCACGGCCGCGTTCCTCTACGCACAACTGGAACACGCCAAGCGAATCAACACCCACAGGCGCGTGTTGTATGAGAAGTA
CCTCCAGTTGCTCGGACCGCTGGAGCAGGAAGGTTTTCTCCAACTGCCGAAGACCGCTGCGCAAGTGGCCAACGGCCATA
TCTTCTACATCCTGTGCACCTCTCTGAAGGAGCGCACCGCCTTGACCGGATTTCTGCGCGCACAGGGTATCGGCGCAGTG
TTCCATTATGTTCCGCTGCATGATTCGCCGGCCGGCCTGCGTCATGGCCGTGGCGGGCACCTGCCGGTCACCACCGATAT
TTCACGGAGGTTGCTGCGCCTGCCCATATATTCCAGCATGACCAGCGACGATGTCCGGCGCGTCGCTGGCGCCGTGAAAA
GCTTCTATCAGCCACACTCCCGCACCGGGGCGTGA

Upstream 100 bases:

>100_bases
GCCATCGACTGGGGGATTCCGCCCGATATGCTCGACATGTACCGGTACGGCCACGCAAAGGCCTGAACCGGGTCCCCGCC
CCTCACGGAAGAGCCCAGCC

Downstream 100 bases:

>100_bases
AGCCCTCCCAGCCCCATGAAACCAGTGCAGTTCATCCGGGATAACCACGCCCGCTTTCTCCACCTGCTGGAACACGTCCG
TCAACTCGTCCAAGCACGGC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDNSSIPFGQPFIVGKELFYIAQAVMNGSIAGDGDFTRKCQEWLEVHLSCKKALLTTSCTTALEMAAILTDVQPGDEIIM
PSFTFVSTANAFVLRGGVPVFVDIRADTLNLDERLVEALITPRTKAVVAVHYAGFPCAMDKLKALCRQHGLRLIEDAAQA
ILTHDEGQALGTIGDMGCLSFHETKNVICGEGGALLINDPALIERAEIIWQKGTNRKAFFRGEVDRYSWVDIGSSFLPNE
LTAAFLYAQLEHAKRINTHRRVLYEKYLQLLGPLEQEGFLQLPKTAAQVANGHIFYILCTSLKERTALTGFLRAQGIGAV
FHYVPLHDSPAGLRHGRGGHLPVTTDISRRLLRLPIYSSMTSDDVRRVAGAVKSFYQPHSRTGA

Sequences:

>Translated_384_residues
MDNSSIPFGQPFIVGKELFYIAQAVMNGSIAGDGDFTRKCQEWLEVHLSCKKALLTTSCTTALEMAAILTDVQPGDEIIM
PSFTFVSTANAFVLRGGVPVFVDIRADTLNLDERLVEALITPRTKAVVAVHYAGFPCAMDKLKALCRQHGLRLIEDAAQA
ILTHDEGQALGTIGDMGCLSFHETKNVICGEGGALLINDPALIERAEIIWQKGTNRKAFFRGEVDRYSWVDIGSSFLPNE
LTAAFLYAQLEHAKRINTHRRVLYEKYLQLLGPLEQEGFLQLPKTAAQVANGHIFYILCTSLKERTALTGFLRAQGIGAV
FHYVPLHDSPAGLRHGRGGHLPVTTDISRRLLRLPIYSSMTSDDVRRVAGAVKSFYQPHSRTGA
>Mature_384_residues
MDNSSIPFGQPFIVGKELFYIAQAVMNGSIAGDGDFTRKCQEWLEVHLSCKKALLTTSCTTALEMAAILTDVQPGDEIIM
PSFTFVSTANAFVLRGGVPVFVDIRADTLNLDERLVEALITPRTKAVVAVHYAGFPCAMDKLKALCRQHGLRLIEDAAQA
ILTHDEGQALGTIGDMGCLSFHETKNVICGEGGALLINDPALIERAEIIWQKGTNRKAFFRGEVDRYSWVDIGSSFLPNE
LTAAFLYAQLEHAKRINTHRRVLYEKYLQLLGPLEQEGFLQLPKTAAQVANGHIFYILCTSLKERTALTGFLRAQGIGAV
FHYVPLHDSPAGLRHGRGGHLPVTTDISRRLLRLPIYSSMTSDDVRRVAGAVKSFYQPHSRTGA

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=374, Percent_Identity=54.0106951871658, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI145693159, Length=378, Percent_Identity=29.1005291005291, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42258; Mature: 42258

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSSIPFGQPFIVGKELFYIAQAVMNGSIAGDGDFTRKCQEWLEVHLSCKKALLTTSCT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TALEMAAILTDVQPGDEIIMPSFTFVSTANAFVLRGGVPVFVDIRADTLNLDERLVEALI
HHHHHHHHHHCCCCCCCEECCCCHHHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHH
TPRTKAVVAVHYAGFPCAMDKLKALCRQHGLRLIEDAAQAILTHDEGQALGTIGDMGCLS
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
FHETKNVICGEGGALLINDPALIERAEIIWQKGTNRKAFFRGEVDRYSWVDIGSSFLPNE
HHHCCCEEECCCCEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCHH
LTAAFLYAQLEHAKRINTHRRVLYEKYLQLLGPLEQEGFLQLPKTAAQVANGHIFYILCT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHCCCCEEEEEEC
SLKERTALTGFLRAQGIGAVFHYVPLHDSPAGLRHGRGGHLPVTTDISRRLLRLPIYSSM
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHCCC
TSDDVRRVAGAVKSFYQPHSRTGA
CHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDNSSIPFGQPFIVGKELFYIAQAVMNGSIAGDGDFTRKCQEWLEVHLSCKKALLTTSCT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TALEMAAILTDVQPGDEIIMPSFTFVSTANAFVLRGGVPVFVDIRADTLNLDERLVEALI
HHHHHHHHHHCCCCCCCEECCCCHHHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHH
TPRTKAVVAVHYAGFPCAMDKLKALCRQHGLRLIEDAAQAILTHDEGQALGTIGDMGCLS
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
FHETKNVICGEGGALLINDPALIERAEIIWQKGTNRKAFFRGEVDRYSWVDIGSSFLPNE
HHHCCCEEECCCCEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCHH
LTAAFLYAQLEHAKRINTHRRVLYEKYLQLLGPLEQEGFLQLPKTAAQVANGHIFYILCT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHCCCCEEEEEEC
SLKERTALTGFLRAQGIGAVFHYVPLHDSPAGLRHGRGGHLPVTTDISRRLLRLPIYSSM
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHCCC
TSDDVRRVAGAVKSFYQPHSRTGA
CHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]