Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 120611806

GI number: 120611806

Start: 3472134

End: 3472508

Strand: Direct

Name: gcvH [H]

Synonym: Aave_3146

Alternate gene names: 120611806

Gene position: 3472134-3472508 (Clockwise)

Preceding gene: 120611805

Following gene: 120611807

Centisome position: 64.87

GC content: 67.2

Gene sequence:

>375_bases
ATGACCGTCAAGTATTCCCGCGACCACGAGTGGATCAACGCCGCCGACACGGGCGCCGCCATCGTCGGCATCACCGTCCA
TGCGCAGGACGCGCTGGGCGACGTGGTGTTCGTCGATCTGCCCGAGGTGGGCAAGACCTTCGCTCAGGGCGAGGTGGCCG
GCGTGGTCGAGTCCGTGAAGGCCGCTGCCGACGTGTACATGCCCGTGTCCGGCGAGATCGTCGAAGTGAACGAGGCGCTG
CGCGCCGATCCGTCGCTCGCCAACAGCGCGCCGCTGGGCGACGGCTGGTTCTTCAAGGTGCGCCTGTCCGATGCGTCGCA
GCTCGACGCGCTCATGGACGAGACGGCCTACACCGAGTTCTCCAAGAACGCCTGA

Upstream 100 bases:

>100_bases
GGCCGCGGCTACAGTCGCAGTCGCGGGCCCCCACCAGGGCCACGCATTCTTTTTTTCGTTTTCCCGCTTTTTTCCGCGGA
CCTTTCCTCCAGGAGCCCCC

Downstream 100 bases:

>100_bases
CCGGCCCCGGCGGCGGGTGGCTTCGGGCCGGCCCGCCGCGGTGCGCCCGCGCCGGGTGCGCCCCTTCCGTTGTTCCTTTT
TTCCCCTTTTCTGTTGACCG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MTVKYSRDHEWINAADTGAAIVGITVHAQDALGDVVFVDLPEVGKTFAQGEVAGVVESVKAAADVYMPVSGEIVEVNEAL
RADPSLANSAPLGDGWFFKVRLSDASQLDALMDETAYTEFSKNA

Sequences:

>Translated_124_residues
MTVKYSRDHEWINAADTGAAIVGITVHAQDALGDVVFVDLPEVGKTFAQGEVAGVVESVKAAADVYMPVSGEIVEVNEAL
RADPSLANSAPLGDGWFFKVRLSDASQLDALMDETAYTEFSKNA
>Mature_123_residues
TVKYSRDHEWINAADTGAAIVGITVHAQDALGDVVFVDLPEVGKTFAQGEVAGVVESVKAAADVYMPVSGEIVEVNEALR
ADPSLANSAPLGDGWFFKVRLSDASQLDALMDETAYTEFSKNA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=46.6666666666667, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=46.6666666666667, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789271, Length=115, Percent_Identity=57.3913043478261, Blast_Score=139, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=43.9655172413793, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=40.3361344537815, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=118, Percent_Identity=42.3728813559322, Blast_Score=111, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=47.008547008547, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13162; Mature: 13031

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKYSRDHEWINAADTGAAIVGITVHAQDALGDVVFVDLPEVGKTFAQGEVAGVVESVK
CCCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHCCHHHHHHHHHH
AAADVYMPVSGEIVEVNEALRADPSLANSAPLGDGWFFKVRLSDASQLDALMDETAYTEF
HHHHEECCCCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHC
SKNA
CCCC
>Mature Secondary Structure 
TVKYSRDHEWINAADTGAAIVGITVHAQDALGDVVFVDLPEVGKTFAQGEVAGVVESVK
CCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHCCHHHHHHHHHH
AAADVYMPVSGEIVEVNEALRADPSLANSAPLGDGWFFKVRLSDASQLDALMDETAYTEF
HHHHEECCCCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHC
SKNA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]