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The map label for this gene is rlmB [H]

Identifier: 120611681

GI number: 120611681

Start: 3327370

End: 3328122

Strand: Reverse

Name: rlmB [H]

Synonym: Aave_3018

Alternate gene names: 120611681

Gene position: 3328122-3327370 (Counterclockwise)

Preceding gene: 120611682

Following gene: 120611680

Centisome position: 62.18

GC content: 70.25

Gene sequence:

>753_bases
ATGTCCTCCAGCCCCAAAGTGCTATTCGGCTTCCACGCCGTGGGCGTGCGCCTGAAGACCGCGCCGCAATCCATCATCGA
GATCTACTACGAGGCCACGCGGCGCGACGCGCGCATGCGCCAGTTCCTCGAGCGCGCGAAGGAGGCGGGAGCGCGCCTCA
TCGAAGCCGATGCGCTGCGCATCGCCAAGCTCGCCGGCAGCCACGGGCACCAGGGCGTGGCCGCCCGCGTGGAAGCCGTC
GCGCAGGTCCATTCGCTCGACGAGCTGCTCGAACAGCTGGAGGCCGACGGCGTCGAGAAGCCGCTGCTGCTGGTGCTCGA
CGGCGTGACCGATCCGCACAACCTGGGCGCCTGCCTGCGCGTGGCCGACGGTGCCGGCGCGCACGCGGTGATCGCGCCCA
AGGACCATGCCGTGGGCATCAATGCCACCGTGGCCAAGGTGGCCAGCGGCGCGGCCGAGACCATGCCGTACTTCATGGTC
ACCAACCTCGCGCGCACCCTGGGCGAGCTCAAGGAGCGCAACATCTGGTGCATCGGCACCAGCGACGACGCCCCGCGCAC
CCTCTACGAAGTGGACCTGAAGGGCCCGGTGGCGCTGGTGCTGGGCGCCGAGGGGGCCGGCATGCGGCAGCTCACGCGCA
AGACCTGCGACGAGCTGGTTTCCATCCCGATGCGGGGAGCGGTGGAAAGCTTGAACGTGTCGGTGGCCAGCGGCGTGTGC
CTCTACGAGGCGCTGCGCCAGCGCGGGGGCTGA

Upstream 100 bases:

>100_bases
ACCATCGACGCAGGCCGGATGGTGGCCGGCGCCTGAGGCGGCCCGGCCTTCTCCGGCTGCCGGAGGGGAAGGGGGCGCCG
GCGGGCGGATAATCGCGCGC

Downstream 100 bases:

>100_bases
TCCGTGACGCAGGAGGCCATGGAACGCCTGCGGTCCTGGGTGGCCGGGGCGCAGCGCATCCTGGTGCTGACCGGCGCCGG
GATGAGCGCGGAATCCGGGG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSSSPKVLFGFHAVGVRLKTAPQSIIEIYYEATRRDARMRQFLERAKEAGARLIEADALRIAKLAGSHGHQGVAARVEAV
AQVHSLDELLEQLEADGVEKPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMV
TNLARTLGELKERNIWCIGTSDDAPRTLYEVDLKGPVALVLGAEGAGMRQLTRKTCDELVSIPMRGAVESLNVSVASGVC
LYEALRQRGG

Sequences:

>Translated_250_residues
MSSSPKVLFGFHAVGVRLKTAPQSIIEIYYEATRRDARMRQFLERAKEAGARLIEADALRIAKLAGSHGHQGVAARVEAV
AQVHSLDELLEQLEADGVEKPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMV
TNLARTLGELKERNIWCIGTSDDAPRTLYEVDLKGPVALVLGAEGAGMRQLTRKTCDELVSIPMRGAVESLNVSVASGVC
LYEALRQRGG
>Mature_249_residues
SSSPKVLFGFHAVGVRLKTAPQSIIEIYYEATRRDARMRQFLERAKEAGARLIEADALRIAKLAGSHGHQGVAARVEAVA
QVHSLDELLEQLEADGVEKPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVT
NLARTLGELKERNIWCIGTSDDAPRTLYEVDLKGPVALVLGAEGAGMRQLTRKTCDELVSIPMRGAVESLNVSVASGVCL
YEALRQRGG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=28.5171102661597, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=49.7942386831276, Blast_Score=233, Evalue=9e-63,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=37.2549019607843, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788935, Length=248, Percent_Identity=25, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=264, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=178, Percent_Identity=32.5842696629214, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26616; Mature: 26485

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSPKVLFGFHAVGVRLKTAPQSIIEIYYEATRRDARMRQFLERAKEAGARLIEADALR
CCCCCCEEEEEHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IAKLAGSHGHQGVAARVEAVAQVHSLDELLEQLEADGVEKPLLLVLDGVTDPHNLGACLR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHEE
VADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVTNLARTLGELKERNIWCIGT
ECCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
SDDAPRTLYEVDLKGPVALVLGAEGAGMRQLTRKTCDELVSIPMRGAVESLNVSVASGVC
CCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHH
LYEALRQRGG
HHHHHHHCCC
>Mature Secondary Structure 
SSSPKVLFGFHAVGVRLKTAPQSIIEIYYEATRRDARMRQFLERAKEAGARLIEADALR
CCCCCEEEEEHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IAKLAGSHGHQGVAARVEAVAQVHSLDELLEQLEADGVEKPLLLVLDGVTDPHNLGACLR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHEE
VADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVTNLARTLGELKERNIWCIGT
ECCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
SDDAPRTLYEVDLKGPVALVLGAEGAGMRQLTRKTCDELVSIPMRGAVESLNVSVASGVC
CCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHH
LYEALRQRGG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]