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The map label for this gene is mdeA [H]

Identifier: 120611660

GI number: 120611660

Start: 3304642

End: 3305979

Strand: Direct

Name: mdeA [H]

Synonym: Aave_2997

Alternate gene names: 120611660

Gene position: 3304642-3305979 (Clockwise)

Preceding gene: 120611657

Following gene: 120611661

Centisome position: 61.74

GC content: 70.7

Gene sequence:

>1338_bases
ATGCCCGGCCATTCCGACCCCGGCTTCGATACGCTGGCGCTGCATGCGGGCGCCGTGCCCGATCCGGCCACCGGCGCAAG
CGCCGTGCCCATCCACCTGACGACCTCGTTCGTCTTCGAATCGAGCGACCATGCGGCGTCGCTGTTCAACCTCGAACGGC
CCGGCCATGTGTACAGCCGGGTCAGCAATCCCACCAACGCGGTGCTGGAGCAGCGCGTGGCGGCGCTCGAAGGCGCCGCC
GGGGCCATCGCGGCGGCGAGCGGCCAGGCCGCGCTGCACCTGGCCATCAGCACGCTGATGGGCGCGGGCGGGCACATCGT
CGCGAGCACGGCGCTCTACGGTGGCTCGCAGAACCTGCTGCACTACACGCTGCGGCGCTTCGGCATCGAGACCACCTTCG
TGCAGCCGGACGACCTGGACGGCTGGCGCGCCGCCATCCGGCCGGAGACGCGGCTGCTGTTCGGAGAGACGGTGGGCAAT
CCGGGCCTGCAGGTGCTGGACATCCCGGCCGTGGCGGAGATCGCCCACGCGGCGGGCGTTCCGCTGCTGGTGGACTCCAC
TCTCACCACCCCCTGGCTCATGCGCCCGCTGGAGCACGGGGCCGACCTGGTCTGTCACTCCGCCACCAAGTTCCTCTCGG
GCCATGGCACGGTGATCGGCGGCGTACTGGTCGATGGGGGCAGCTTCGACTGGGAGGCCTCGGGGCGCTTCCCGGAACTC
TGCGCGCCGTACGCGGGCTTCCACGGCATGGTGTTCTCGGAGGAGTCCACCGTGGGTGCCCTCCTGCTGCGCGCGCGGCG
CGAGGGGCTGCGCGATTTCGGCGCGTGCCTGAGCCCGCACACGGCCTGGCTGATCCTGCAGGGCATCGAAACCCTGCCGC
TGCGCATGGAACGGCACATGCGCAACACGGAGCGGGTGGTGGAGTTCCTGGCGAGCCACCCCTTCGTGTCCCGCGTCGGC
CACCCGATGCTGGAGTCGCACCCCAGCCATGCACTCGCGAAGCGCCTGCTGCCGCGCGGCGCAGGCTCGGTGTTCAGCTT
CGATCTGCGCGGCGACCGGGCCCAGGGCCGGGCCTTCATCGAGTCGCTGAAGGTCTTCAGCCACCTCGCCAACGTGGGCG
ATTGCCGCAGCCTGGTGATCCACCCCGCCAGCACGACGCACTTCCGGATGGACGATGCGGCGCTGGCGCAGTCCGGCATC
GCACAGGGCACGATCCGCCTGTCGATCGGACTGGAGGATGCGGACGACCTGATCGACGACCTGAAACGCGCGCTCAAGTC
CGCGGAGAAAGCAGGCGCCCGGACTGGCGACCGAGCCGGCGGAAAGGCGGGTGCGTGA

Upstream 100 bases:

>100_bases
GGTCTCCAGGGGGCGCCGCGCCGCTGCGGCGGGCCGATCGATGTTTCCTGCGCAATATAGCCCACAATGTTGCGCTGCGA
TCACCCATAGGAGATAACGA

Downstream 100 bases:

>100_bases
TGCAGGTGACCGTCAACGGCCACGCGGCCTATTGCTATACGGGCAGCAAGCCCTTCGACCCGGCACTGCCCACGGTGGTG
ATGGTGCATGGCGTGCTCAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPGHSDPGFDTLALHAGAVPDPATGASAVPIHLTTSFVFESSDHAASLFNLERPGHVYSRVSNPTNAVLEQRVAALEGAA
GAIAAASGQAALHLAISTLMGAGGHIVASTALYGGSQNLLHYTLRRFGIETTFVQPDDLDGWRAAIRPETRLLFGETVGN
PGLQVLDIPAVAEIAHAAGVPLLVDSTLTTPWLMRPLEHGADLVCHSATKFLSGHGTVIGGVLVDGGSFDWEASGRFPEL
CAPYAGFHGMVFSEESTVGALLLRARREGLRDFGACLSPHTAWLILQGIETLPLRMERHMRNTERVVEFLASHPFVSRVG
HPMLESHPSHALAKRLLPRGAGSVFSFDLRGDRAQGRAFIESLKVFSHLANVGDCRSLVIHPASTTHFRMDDAALAQSGI
AQGTIRLSIGLEDADDLIDDLKRALKSAEKAGARTGDRAGGKAGA

Sequences:

>Translated_445_residues
MPGHSDPGFDTLALHAGAVPDPATGASAVPIHLTTSFVFESSDHAASLFNLERPGHVYSRVSNPTNAVLEQRVAALEGAA
GAIAAASGQAALHLAISTLMGAGGHIVASTALYGGSQNLLHYTLRRFGIETTFVQPDDLDGWRAAIRPETRLLFGETVGN
PGLQVLDIPAVAEIAHAAGVPLLVDSTLTTPWLMRPLEHGADLVCHSATKFLSGHGTVIGGVLVDGGSFDWEASGRFPEL
CAPYAGFHGMVFSEESTVGALLLRARREGLRDFGACLSPHTAWLILQGIETLPLRMERHMRNTERVVEFLASHPFVSRVG
HPMLESHPSHALAKRLLPRGAGSVFSFDLRGDRAQGRAFIESLKVFSHLANVGDCRSLVIHPASTTHFRMDDAALAQSGI
AQGTIRLSIGLEDADDLIDDLKRALKSAEKAGARTGDRAGGKAGA
>Mature_444_residues
PGHSDPGFDTLALHAGAVPDPATGASAVPIHLTTSFVFESSDHAASLFNLERPGHVYSRVSNPTNAVLEQRVAALEGAAG
AIAAASGQAALHLAISTLMGAGGHIVASTALYGGSQNLLHYTLRRFGIETTFVQPDDLDGWRAAIRPETRLLFGETVGNP
GLQVLDIPAVAEIAHAAGVPLLVDSTLTTPWLMRPLEHGADLVCHSATKFLSGHGTVIGGVLVDGGSFDWEASGRFPELC
APYAGFHGMVFSEESTVGALLLRARREGLRDFGACLSPHTAWLILQGIETLPLRMERHMRNTERVVEFLASHPFVSRVGH
PMLESHPSHALAKRLLPRGAGSVFSFDLRGDRAQGRAFIESLKVFSHLANVGDCRSLVIHPASTTHFRMDDAALAQSGIA
QGTIRLSIGLEDADDLIDDLKRALKSAEKAGARTGDRAGGKAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.9881796690307, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=32.1513002364066, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=31.042654028436, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=32.6190476190476, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=26.8235294117647, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=405, Percent_Identity=32.0987654320988, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=33.7236533957845, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=33.2552693208431, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=441, Percent_Identity=42.8571428571429, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=33.9578454332553, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=25.1111111111111, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=27.4011299435028, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=27.6102088167053, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=35.8313817330211, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47070; Mature: 46939

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGHSDPGFDTLALHAGAVPDPATGASAVPIHLTTSFVFESSDHAASLFNLERPGHVYSR
CCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHCCCCCCHHHHH
VSNPTNAVLEQRVAALEGAAGAIAAASGQAALHLAISTLMGAGGHIVASTALYGGSQNLL
CCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEHEEECCCCHHH
HYTLRRFGIETTFVQPDDLDGWRAAIRPETRLLFGETVGNPGLQVLDIPAVAEIAHAAGV
HHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCCCCCEEEECHHHHHHHHHCCC
PLLVDSTLTTPWLMRPLEHGADLVCHSATKFLSGHGTVIGGVLVDGGSFDWEASGRFPEL
CEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHH
CAPYAGFHGMVFSEESTVGALLLRARREGLRDFGACLSPHTAWLILQGIETLPLRMERHM
HCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHHHHHH
RNTERVVEFLASHPFVSRVGHPMLESHPSHALAKRLLPRGAGSVFSFDLRGDRAQGRAFI
HHHHHHHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
ESLKVFSHLANVGDCRSLVIHPASTTHFRMDDAALAQSGIAQGTIRLSIGLEDADDLIDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEECCHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKRALKSAEKAGARTGDRAGGKAGA
HHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGHSDPGFDTLALHAGAVPDPATGASAVPIHLTTSFVFESSDHAASLFNLERPGHVYSR
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHCCCCCCHHHHH
VSNPTNAVLEQRVAALEGAAGAIAAASGQAALHLAISTLMGAGGHIVASTALYGGSQNLL
CCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEHEEECCCCHHH
HYTLRRFGIETTFVQPDDLDGWRAAIRPETRLLFGETVGNPGLQVLDIPAVAEIAHAAGV
HHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCCCCCEEEECHHHHHHHHHCCC
PLLVDSTLTTPWLMRPLEHGADLVCHSATKFLSGHGTVIGGVLVDGGSFDWEASGRFPEL
CEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHH
CAPYAGFHGMVFSEESTVGALLLRARREGLRDFGACLSPHTAWLILQGIETLPLRMERHM
HCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHHHHHH
RNTERVVEFLASHPFVSRVGHPMLESHPSHALAKRLLPRGAGSVFSFDLRGDRAQGRAFI
HHHHHHHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
ESLKVFSHLANVGDCRSLVIHPASTTHFRMDDAALAQSGIAQGTIRLSIGLEDADDLIDD
HHHHHHHHHHCCCCCCEEEEECCCCCEEECCHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKRALKSAEKAGARTGDRAGGKAGA
HHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]