Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 120611646

GI number: 120611646

Start: 3275225

End: 3277381

Strand: Direct

Name: glgX [H]

Synonym: Aave_2983

Alternate gene names: 120611646

Gene position: 3275225-3277381 (Clockwise)

Preceding gene: 120611645

Following gene: 120611647

Centisome position: 61.19

GC content: 69.96

Gene sequence:

>2157_bases
ATGAAGGCAGGCGACGCCGCACCGCACGTGACCTCCCGCCGCCCGCGCGCCACCCGGCACACCGCCGAGGCCGGCCGGCC
GCTGCCCCTGGGCGCCACGGCCGACCGCAACGGCGTGAACTTCGCCGTGCACGCGCCGGGCGCGGAACGCGTCGAAGTCT
GCCTCTTCGCCGATGGCGAGAACGAGACGCAGCGCATCGCCCTGCCGGCATTCACCGCCGGCACGTGGCACGGGCACGTC
AAGGGAATCAAGCCGGGCCAGCGCTACGGCCTGCGAGTCCATGGACCCTACGCCCCTGAGAAGGGGCAGCGCTTCAATCC
CGCCAAGCTGCTCATCGATCCGTACGCCCGCGCGCTCGACCGCCCCGTGCTCGGCGCCGACGACCAGTTCGGCTACGAAC
TCGGCCACGAGGACGAGGACCATGCCGTCAGCAGCGTGGACAACGGTGCCACAGCGCCCAAGGCCATCGTGGTCCGCTCG
CGCTTCGACTGGGACGACGACGTCCCGCCCCGCGTTCCGCCCTCCAGGACCGTCTTCTATGAAATGCACGTTAAGGGCTT
CACGCAGACGCTGTCCGGCGTTCCGGAAGGCGAGCGCGGCACCTATGCCGGGCTGGCATCGGAAGCCGCCATCGCCTACC
TGAAGGACCTGGGCGTGACCAGCGTGGAACTGCTGCCCGTGCAGGCCTTCGTGGACGACAAGCGGCTCGTGGACGCGGGC
CTGGCCAACTACTGGGGCTACAACACCATCGCCTTCTTCGCGCCCGAGCCCCGCTACGCCGCCAGCCGCGGCGACGGCGG
CACGGACGAGTTCAAGGCCATGGTCAGGGCCCTGCACGCCGCCGGCATCGAGGTGATCCTGGACGTGGTGTACAACCACA
CCGCCGAAGGCAACCACCTGGGCCCCACGCTCAGCCTCAAGGGCATCGACAACGCCGCCTACTACCGCCTCTCCCCGGAA
GATCCGCGCTTTTACGTGGACTACACCGGCACGGGGAACACCGTGGACACGAGCAGCCCCGCCGCCCTGCGCCTGGTCAT
GGACAGCCTGCGCTACTGGGTCACGGAGATGCACGTGGACGGCTTCCGCTTCGACCTGGCCTGCTCGCTGGGGCGCGACG
TTTCGGGCGCCTTCACCCACCGCGCGGCCTTCTTCGCCGCGGTGGCGCAGGATCCCGTGCTCTCGCGCGTCAAGCTCATC
GCCGAACCCTGGGACCTGGGCCCCGACGGCTACCAGGTGGGCGGCTTCCCCGACGGCTGGATGGAATGGAACGGTCGCTA
CCGCGACGTGGTGCGCGACTACTGGCGCGGCACCGACGGCAGCCTGCCCGCATTCGCCGCCTGCCTCTGCGGCAGTGCCG
ACATCCTGGAAGCACGCCGCCGCCCGGCGACCGACAGCATCAACATCGTCACCGTGCACGACGGCTTCACCCTGGCCGAC
CTCGTCAGCTACAACGAAAAGCACAACGAGGCCAACCAGGAAGACAGCCGGGACGGCGAAAACCACAACCGCAGCTGGAA
CTGCGGCGCCGAAGGCCCGACCGAGGACCCCGCCATCCTGGACCTGCGCGAGCGCCAGATGCGCAACTTTCTCGCCACGC
TCTTCGTGTCGCACGGTACGCCGCTGCTGCTGGGCGGCGACGAGCATGCCCGCACCCAGCAGGGCAACAACAACGGCTAC
TGCCAGGACAGCCCCCTGTCGTGGTACGACTGGGAGCACGCCGGCCGCAACGACGCCCAGCGCCGCTTCACCGCGGCCCT
GATCGCCCTGCGCTCCGAACTGCCGGCGCTGCGCACCGCGGTAGCCGACAGCGCCCGGGCCGACTGCGTGGGCGTGCACT
GGCACAGCGTCTGGGGCCTGCCGATGACGGCGGAAGAATGGGACGACCCGCAATCGCGCTGCGTCGCCGCCCTGATGGAG
GCGGATGCCGGCGGCTGCGCGGCGCTCGTCCTCTTCAACGCCACCGCCGAAGACGCGACCTTCACCCTCCCGCAGGAGGA
GCAGGCCCGTTCCTGGACCGTGCGCGTGGACACGCGCACCGCCGACGTGCCACCCGCAGATACCGAGCCTGTAGCTTCCG
GAGGCCAATACGTGCTGCCGGGGCATTCCATGGCGATCCTCACCGCATTGGGCGCTTCCGGCGACCCCGCTCCATGA

Upstream 100 bases:

>100_bases
TCTTCAAGCCGCAGAGCCTGCAGTCCACCACCCCCAGCATCGGACGGGCACGCCCCTCCGTGGCGTCGGCATCCGAGGGC
TCCGGCCTGAAGGTGGCCGC

Downstream 100 bases:

>100_bases
AGCACCAGCACGACATGCCGATCGGCGCCCGCCTCCTTCCTGAAGGCGGCGTCCACTTCACCCTCTGGGCCCCCGCCGCC
TCGCGGCTCACCCTCCACCA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKAGDAAPHVTSRRPRATRHTAEAGRPLPLGATADRNGVNFAVHAPGAERVEVCLFADGENETQRIALPAFTAGTWHGHV
KGIKPGQRYGLRVHGPYAPEKGQRFNPAKLLIDPYARALDRPVLGADDQFGYELGHEDEDHAVSSVDNGATAPKAIVVRS
RFDWDDDVPPRVPPSRTVFYEMHVKGFTQTLSGVPEGERGTYAGLASEAAIAYLKDLGVTSVELLPVQAFVDDKRLVDAG
LANYWGYNTIAFFAPEPRYAASRGDGGTDEFKAMVRALHAAGIEVILDVVYNHTAEGNHLGPTLSLKGIDNAAYYRLSPE
DPRFYVDYTGTGNTVDTSSPAALRLVMDSLRYWVTEMHVDGFRFDLACSLGRDVSGAFTHRAAFFAAVAQDPVLSRVKLI
AEPWDLGPDGYQVGGFPDGWMEWNGRYRDVVRDYWRGTDGSLPAFAACLCGSADILEARRRPATDSINIVTVHDGFTLAD
LVSYNEKHNEANQEDSRDGENHNRSWNCGAEGPTEDPAILDLRERQMRNFLATLFVSHGTPLLLGGDEHARTQQGNNNGY
CQDSPLSWYDWEHAGRNDAQRRFTAALIALRSELPALRTAVADSARADCVGVHWHSVWGLPMTAEEWDDPQSRCVAALME
ADAGGCAALVLFNATAEDATFTLPQEEQARSWTVRVDTRTADVPPADTEPVASGGQYVLPGHSMAILTALGASGDPAP

Sequences:

>Translated_718_residues
MKAGDAAPHVTSRRPRATRHTAEAGRPLPLGATADRNGVNFAVHAPGAERVEVCLFADGENETQRIALPAFTAGTWHGHV
KGIKPGQRYGLRVHGPYAPEKGQRFNPAKLLIDPYARALDRPVLGADDQFGYELGHEDEDHAVSSVDNGATAPKAIVVRS
RFDWDDDVPPRVPPSRTVFYEMHVKGFTQTLSGVPEGERGTYAGLASEAAIAYLKDLGVTSVELLPVQAFVDDKRLVDAG
LANYWGYNTIAFFAPEPRYAASRGDGGTDEFKAMVRALHAAGIEVILDVVYNHTAEGNHLGPTLSLKGIDNAAYYRLSPE
DPRFYVDYTGTGNTVDTSSPAALRLVMDSLRYWVTEMHVDGFRFDLACSLGRDVSGAFTHRAAFFAAVAQDPVLSRVKLI
AEPWDLGPDGYQVGGFPDGWMEWNGRYRDVVRDYWRGTDGSLPAFAACLCGSADILEARRRPATDSINIVTVHDGFTLAD
LVSYNEKHNEANQEDSRDGENHNRSWNCGAEGPTEDPAILDLRERQMRNFLATLFVSHGTPLLLGGDEHARTQQGNNNGY
CQDSPLSWYDWEHAGRNDAQRRFTAALIALRSELPALRTAVADSARADCVGVHWHSVWGLPMTAEEWDDPQSRCVAALME
ADAGGCAALVLFNATAEDATFTLPQEEQARSWTVRVDTRTADVPPADTEPVASGGQYVLPGHSMAILTALGASGDPAP
>Mature_718_residues
MKAGDAAPHVTSRRPRATRHTAEAGRPLPLGATADRNGVNFAVHAPGAERVEVCLFADGENETQRIALPAFTAGTWHGHV
KGIKPGQRYGLRVHGPYAPEKGQRFNPAKLLIDPYARALDRPVLGADDQFGYELGHEDEDHAVSSVDNGATAPKAIVVRS
RFDWDDDVPPRVPPSRTVFYEMHVKGFTQTLSGVPEGERGTYAGLASEAAIAYLKDLGVTSVELLPVQAFVDDKRLVDAG
LANYWGYNTIAFFAPEPRYAASRGDGGTDEFKAMVRALHAAGIEVILDVVYNHTAEGNHLGPTLSLKGIDNAAYYRLSPE
DPRFYVDYTGTGNTVDTSSPAALRLVMDSLRYWVTEMHVDGFRFDLACSLGRDVSGAFTHRAAFFAAVAQDPVLSRVKLI
AEPWDLGPDGYQVGGFPDGWMEWNGRYRDVVRDYWRGTDGSLPAFAACLCGSADILEARRRPATDSINIVTVHDGFTLAD
LVSYNEKHNEANQEDSRDGENHNRSWNCGAEGPTEDPAILDLRERQMRNFLATLFVSHGTPLLLGGDEHARTQQGNNNGY
CQDSPLSWYDWEHAGRNDAQRRFTAALIALRSELPALRTAVADSARADCVGVHWHSVWGLPMTAEEWDDPQSRCVAALME
ADAGGCAALVLFNATAEDATFTLPQEEQARSWTVRVDTRTADVPPADTEPVASGGQYVLPGHSMAILTALGASGDPAP

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=649, Percent_Identity=45.7627118644068, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78139; Mature: 78139

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGDAAPHVTSRRPRATRHTAEAGRPLPLGATADRNGVNFAVHAPGAERVEVCLFADGE
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCC
NETQRIALPAFTAGTWHGHVKGIKPGQRYGLRVHGPYAPEKGQRFNPAKLLIDPYARALD
CCCEEEEEEEEECCCCCCEECCCCCCHHCCEEEECCCCCCCCCCCCCHHEEECHHHHHHC
RPVLGADDQFGYELGHEDEDHAVSSVDNGATAPKAIVVRSRFDWDDDVPPRVPPSRTVFY
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
EMHVKGFTQTLSGVPEGERGTYAGLASEAAIAYLKDLGVTSVELLPVQAFVDDKRLVDAG
EEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCHHHHHHH
LANYWGYNTIAFFAPEPRYAASRGDGGTDEFKAMVRALHAAGIEVILDVVYNHTAEGNHL
HHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GPTLSLKGIDNAAYYRLSPEDPRFYVDYTGTGNTVDTSSPAALRLVMDSLRYWVTEMHVD
CCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GFRFDLACSLGRDVSGAFTHRAAFFAAVAQDPVLSRVKLIAEPWDLGPDGYQVGGFPDGW
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCH
MEWNGRYRDVVRDYWRGTDGSLPAFAACLCGSADILEARRRPATDSINIVTVHDGFTLAD
HCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LVSYNEKHNEANQEDSRDGENHNRSWNCGAEGPTEDPAILDLRERQMRNFLATLFVSHGT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PLLLGGDEHARTQQGNNNGYCQDSPLSWYDWEHAGRNDAQRRFTAALIALRSELPALRTA
EEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
VADSARADCVGVHWHSVWGLPMTAEEWDDPQSRCVAALMEADAGGCAALVLFNATAEDAT
HHCCCCCCEEEEEEHHCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCE
FTLPQEEQARSWTVRVDTRTADVPPADTEPVASGGQYVLPGHSMAILTALGASGDPAP
EECCCHHCCCEEEEEEECEECCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKAGDAAPHVTSRRPRATRHTAEAGRPLPLGATADRNGVNFAVHAPGAERVEVCLFADGE
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCC
NETQRIALPAFTAGTWHGHVKGIKPGQRYGLRVHGPYAPEKGQRFNPAKLLIDPYARALD
CCCEEEEEEEEECCCCCCEECCCCCCHHCCEEEECCCCCCCCCCCCCHHEEECHHHHHHC
RPVLGADDQFGYELGHEDEDHAVSSVDNGATAPKAIVVRSRFDWDDDVPPRVPPSRTVFY
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
EMHVKGFTQTLSGVPEGERGTYAGLASEAAIAYLKDLGVTSVELLPVQAFVDDKRLVDAG
EEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCHHHHHHH
LANYWGYNTIAFFAPEPRYAASRGDGGTDEFKAMVRALHAAGIEVILDVVYNHTAEGNHL
HHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GPTLSLKGIDNAAYYRLSPEDPRFYVDYTGTGNTVDTSSPAALRLVMDSLRYWVTEMHVD
CCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GFRFDLACSLGRDVSGAFTHRAAFFAAVAQDPVLSRVKLIAEPWDLGPDGYQVGGFPDGW
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCH
MEWNGRYRDVVRDYWRGTDGSLPAFAACLCGSADILEARRRPATDSINIVTVHDGFTLAD
HCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LVSYNEKHNEANQEDSRDGENHNRSWNCGAEGPTEDPAILDLRERQMRNFLATLFVSHGT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PLLLGGDEHARTQQGNNNGYCQDSPLSWYDWEHAGRNDAQRRFTAALIALRSELPALRTA
EEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
VADSARADCVGVHWHSVWGLPMTAEEWDDPQSRCVAALMEADAGGCAALVLFNATAEDAT
HHCCCCCCEEEEEEHHCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCE
FTLPQEEQARSWTVRVDTRTADVPPADTEPVASGGQYVLPGHSMAILTALGASGDPAP
EECCCHHCCCEEEEEEECEECCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]