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The map label for this gene is alkK [H]

Identifier: 120611609

GI number: 120611609

Start: 3235819

End: 3237459

Strand: Reverse

Name: alkK [H]

Synonym: Aave_2946

Alternate gene names: 120611609

Gene position: 3237459-3235819 (Counterclockwise)

Preceding gene: 120611615

Following gene: 120611608

Centisome position: 60.48

GC content: 66.91

Gene sequence:

>1641_bases
ATGCTGGGCCTGATGCAGAGCCAACCCCTGTTGATTTCGACGCTCGTCGAGTTCGCGGAACGCCACCATGGTGATGCGCA
GATCGTCTCGCGGCGCGTGGAGGGCGACATCCACCGCTACACATACCGGGACATGGCAGCCCGTGCGCGCCAGCTCGCCA
ACACGCTGGATGCGGAGGGCCTCGCCGCCAGCGCCCGCGTCGCCAGCCTGGCATGGAACGGCTACCGCCACCTGGAGATG
TATTACGGGGTGAGCGGTTCTGGCCGCGTGCTGCACACGATCAATCCGCGGCTGCATCCCGACCAGGTCGCCTGGATCGT
GAACCATGCGGAAGACGAAGTGCTGTGCTTCGACCTCACGTTCCTGCCCATCGTGCAGGCGGTGCACGCGAAATGCCCCT
CGGTCCGGCGCTGGGTGGCGCTTTGCGATGCCGACCGGTTGCCGGAGGGCACGGGTATTCCGCAGCTCACGAGCTACGAG
AGCTGGATCGGCGCGGCCTCCAGCGACTACGCCTGGCCGGAGTTCGACGAGAACTCCGCGTCCAGCCTCTGCTACACCAG
CGGCACCACGGGCCACCCCAAGGGCGTGCTCTACAGCCACCGCTCCTCGGTCCTGCATGCCTACGCCGCGGCACTGCCGG
ACGTGATGGGCCTTTCGGCCAGCGATTCGGTGCTCCCCGTGGTGCCCATGTTCCACGTCAACGCCTGGGGCATCCCGTAT
TCGGCGCCGCTCACCGGGTGCAAGCTGGTCTTCCCCGGCCCCGCGCTGGACGGGAAGTCCGTCTATGACCTCATGGACGC
CGAAGGCGTGACCTTCGCGGCCGGCGTACCGACGGTCTGGCAGATGCTGCTGCAGCACGTCAGATCCATCGGCGCGCGTT
TCGGCAAGCTCCGGCGCACGGTGATCGGCGGCTCCGCGTGCCCGCCCGCGATGATCGAGGCCTTCCAGAACGAGTACGGC
GTGCGCGTGCTGCATGCCTGGGGCATGACCGAGATGAGTCCCCTGGGCACGCTCTGCTCGCTCAAGAACAAGCACCTGGA
CCTGCCGGAGCAGGAGCGCATGAAGATCCTGCTCAAGCAGGGCCGCGCCATCTACGGCGTCGACATGAAAATCGTCGATG
GCGAAGGGCGCGAGCTGCCCTGGGACGGCAAGACCTACGGCGACCTGCTGGTCAAGGGGCCCTGGATCGTGGACCGCTAC
TACCGCTCCGACGAGAGCCCCCTGGTGCCCGACGCGCAGGGCCGCGGCTGGTTCCCGACGGGCGACGTGGCGACCATCGA
CCCCGACGGCTACATGCAGATCACGGACCGCAGCAAGGACGTGATCAAGTCCGGTGGCGAATGGATCAGCTCCATCGACA
TCGAGAACATCGCCATGGCGCATCCAGCCGTGGCCATGGCGGCATGCGTGGGCATGCCGCATCCGAAGTGGGACGAGCGA
CCCATCGTGGCCGTCGTGCGGCGCCAGGGCGCAGAACTCACGCGCGAGGAACTGTTGGCCTTCTACGAGGGGAAGACCGC
GAAATGGCAGATTCCGGACGATGTCGTGTTCCTCGATGCGATCCCCATCGGCGCGACCGGGAAGATGCTCAAGGCGAAGC
TGCGCGACCAGTTGCAGGGCTACCGCCTGCCCGGGCTCTGA

Upstream 100 bases:

>100_bases
ACAAGGGCCGCCGGACGCGGGGTTGCCCTGGGGGCGCGGCACCGTACAGCAGGGTTAGGATCCGGTGATCACCACATCCA
CGGGATTGAAGGAGACAAAC

Downstream 100 bases:

>100_bases
GCGCGGCAGGTGCGGCGCGCGGCGCGGGCGGCGCGCCGCCGCGAGTCGCATCCGGGATAGACGCTAGGGTGAATCCCTGA
ACACCGCCGCGGAGCTCGCC

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MLGLMQSQPLLISTLVEFAERHHGDAQIVSRRVEGDIHRYTYRDMAARARQLANTLDAEGLAASARVASLAWNGYRHLEM
YYGVSGSGRVLHTINPRLHPDQVAWIVNHAEDEVLCFDLTFLPIVQAVHAKCPSVRRWVALCDADRLPEGTGIPQLTSYE
SWIGAASSDYAWPEFDENSASSLCYTSGTTGHPKGVLYSHRSSVLHAYAAALPDVMGLSASDSVLPVVPMFHVNAWGIPY
SAPLTGCKLVFPGPALDGKSVYDLMDAEGVTFAAGVPTVWQMLLQHVRSIGARFGKLRRTVIGGSACPPAMIEAFQNEYG
VRVLHAWGMTEMSPLGTLCSLKNKHLDLPEQERMKILLKQGRAIYGVDMKIVDGEGRELPWDGKTYGDLLVKGPWIVDRY
YRSDESPLVPDAQGRGWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWISSIDIENIAMAHPAVAMAACVGMPHPKWDER
PIVAVVRRQGAELTREELLAFYEGKTAKWQIPDDVVFLDAIPIGATGKMLKAKLRDQLQGYRLPGL

Sequences:

>Translated_546_residues
MLGLMQSQPLLISTLVEFAERHHGDAQIVSRRVEGDIHRYTYRDMAARARQLANTLDAEGLAASARVASLAWNGYRHLEM
YYGVSGSGRVLHTINPRLHPDQVAWIVNHAEDEVLCFDLTFLPIVQAVHAKCPSVRRWVALCDADRLPEGTGIPQLTSYE
SWIGAASSDYAWPEFDENSASSLCYTSGTTGHPKGVLYSHRSSVLHAYAAALPDVMGLSASDSVLPVVPMFHVNAWGIPY
SAPLTGCKLVFPGPALDGKSVYDLMDAEGVTFAAGVPTVWQMLLQHVRSIGARFGKLRRTVIGGSACPPAMIEAFQNEYG
VRVLHAWGMTEMSPLGTLCSLKNKHLDLPEQERMKILLKQGRAIYGVDMKIVDGEGRELPWDGKTYGDLLVKGPWIVDRY
YRSDESPLVPDAQGRGWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWISSIDIENIAMAHPAVAMAACVGMPHPKWDER
PIVAVVRRQGAELTREELLAFYEGKTAKWQIPDDVVFLDAIPIGATGKMLKAKLRDQLQGYRLPGL
>Mature_546_residues
MLGLMQSQPLLISTLVEFAERHHGDAQIVSRRVEGDIHRYTYRDMAARARQLANTLDAEGLAASARVASLAWNGYRHLEM
YYGVSGSGRVLHTINPRLHPDQVAWIVNHAEDEVLCFDLTFLPIVQAVHAKCPSVRRWVALCDADRLPEGTGIPQLTSYE
SWIGAASSDYAWPEFDENSASSLCYTSGTTGHPKGVLYSHRSSVLHAYAAALPDVMGLSASDSVLPVVPMFHVNAWGIPY
SAPLTGCKLVFPGPALDGKSVYDLMDAEGVTFAAGVPTVWQMLLQHVRSIGARFGKLRRTVIGGSACPPAMIEAFQNEYG
VRVLHAWGMTEMSPLGTLCSLKNKHLDLPEQERMKILLKQGRAIYGVDMKIVDGEGRELPWDGKTYGDLLVKGPWIVDRY
YRSDESPLVPDAQGRGWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWISSIDIENIAMAHPAVAMAACVGMPHPKWDER
PIVAVVRRQGAELTREELLAFYEGKTAKWQIPDDVVFLDAIPIGATGKMLKAKLRDQLQGYRLPGL

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=25.5238095238095, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=25.1838235294118, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=25.1838235294118, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI38505220, Length=430, Percent_Identity=26.7441860465116, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=25.0489236790607, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=25.0489236790607, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=25.0489236790607, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=23.4615384615385, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=23.5867446393762, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI115511026, Length=524, Percent_Identity=22.9007633587786, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI28416953, Length=549, Percent_Identity=23.3151183970856, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI13375727, Length=396, Percent_Identity=24.7474747474747, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8923896, Length=405, Percent_Identity=25.1851851851852, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI253970489, Length=405, Percent_Identity=25.1851851851852, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI221142682, Length=509, Percent_Identity=25.5402750491159, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1788107, Length=528, Percent_Identity=25.7575757575758, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693145, Length=510, Percent_Identity=26.6666666666667, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=26.9444444444444, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786810, Length=359, Percent_Identity=27.2980501392758, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788595, Length=340, Percent_Identity=24.7058823529412, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790505, Length=397, Percent_Identity=24.9370277078086, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=534, Percent_Identity=24.9063670411985, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=534, Percent_Identity=24.9063670411985, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=534, Percent_Identity=24.9063670411985, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=363, Percent_Identity=28.3746556473829, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=375, Percent_Identity=27.7333333333333, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=530, Percent_Identity=26.0377358490566, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=434, Percent_Identity=26.9585253456221, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=24.0986717267552, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=23.21083172147, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=21.2475633528265, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=357, Percent_Identity=24.6498599439776, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=509, Percent_Identity=21.2180746561886, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996755, Length=377, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=389, Percent_Identity=26.2210796915167, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=389, Percent_Identity=26.2210796915167, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=29.1666666666667, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24581924, Length=560, Percent_Identity=25.3571428571429, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859661, Length=468, Percent_Identity=28.6324786324786, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=22.9601518026565, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922652, Length=466, Percent_Identity=24.2489270386266, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648260, Length=372, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24656500, Length=506, Percent_Identity=22.5296442687747, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356947, Length=358, Percent_Identity=24.3016759776536, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=378, Percent_Identity=23.015873015873, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648257, Length=533, Percent_Identity=21.5759849906191, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=21.4574898785425, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281365686, Length=256, Percent_Identity=23.4375, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=361, Percent_Identity=22.4376731301939, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648255, Length=494, Percent_Identity=21.2550607287449, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 60106; Mature: 60106

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQSQPLLISTLVEFAERHHGDAQIVSRRVEGDIHRYTYRDMAARARQLANTLDAEG
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
LAASARVASLAWNGYRHLEMYYGVSGSGRVLHTINPRLHPDQVAWIVNHAEDEVLCFDLT
CHHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEECH
FLPIVQAVHAKCPSVRRWVALCDADRLPEGTGIPQLTSYESWIGAASSDYAWPEFDENSA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
SSLCYTSGTTGHPKGVLYSHRSSVLHAYAAALPDVMGLSASDSVLPVVPMFHVNAWGIPY
CCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCC
SAPLTGCKLVFPGPALDGKSVYDLMDAEGVTFAAGVPTVWQMLLQHVRSIGARFGKLRRT
CCCCCCCEEEECCCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
VIGGSACPPAMIEAFQNEYGVRVLHAWGMTEMSPLGTLCSLKNKHLDLPEQERMKILLKQ
HCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHC
GRAIYGVDMKIVDGEGRELPWDGKTYGDLLVKGPWIVDRYYRSDESPLVPDAQGRGWFPT
CCEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCEEEHHHHCCCCCCCCCCCCCCCCCCC
GDVATIDPDGYMQITDRSKDVIKSGGEWISSIDIENIAMAHPAVAMAACVGMPHPKWDER
CCEEEECCCCCEEECCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PIVAVVRRQGAELTREELLAFYEGKTAKWQIPDDVVFLDAIPIGATGKMLKAKLRDQLQG
HHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCC
YRLPGL
CCCCCC
>Mature Secondary Structure
MLGLMQSQPLLISTLVEFAERHHGDAQIVSRRVEGDIHRYTYRDMAARARQLANTLDAEG
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
LAASARVASLAWNGYRHLEMYYGVSGSGRVLHTINPRLHPDQVAWIVNHAEDEVLCFDLT
CHHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEECH
FLPIVQAVHAKCPSVRRWVALCDADRLPEGTGIPQLTSYESWIGAASSDYAWPEFDENSA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
SSLCYTSGTTGHPKGVLYSHRSSVLHAYAAALPDVMGLSASDSVLPVVPMFHVNAWGIPY
CCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCC
SAPLTGCKLVFPGPALDGKSVYDLMDAEGVTFAAGVPTVWQMLLQHVRSIGARFGKLRRT
CCCCCCCEEEECCCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
VIGGSACPPAMIEAFQNEYGVRVLHAWGMTEMSPLGTLCSLKNKHLDLPEQERMKILLKQ
HCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHC
GRAIYGVDMKIVDGEGRELPWDGKTYGDLLVKGPWIVDRYYRSDESPLVPDAQGRGWFPT
CCEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCEEEHHHHCCCCCCCCCCCCCCCCCCC
GDVATIDPDGYMQITDRSKDVIKSGGEWISSIDIENIAMAHPAVAMAACVGMPHPKWDER
CCEEEECCCCCEEECCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
PIVAVVRRQGAELTREELLAFYEGKTAKWQIPDDVVFLDAIPIGATGKMLKAKLRDQLQG
HHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCC
YRLPGL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]