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The map label for this gene is pheS

Identifier: 120611519

GI number: 120611519

Start: 3146934

End: 3147986

Strand: Reverse

Name: pheS

Synonym: Aave_2855

Alternate gene names: 120611519

Gene position: 3147986-3146934 (Counterclockwise)

Preceding gene: 120611520

Following gene: 120611518

Centisome position: 58.81

GC content: 66.67

Gene sequence:

>1053_bases
ATGAACGAGCTGGATTCCCTGGTAGATGCCGCCCGCAGGATGTTCGCGGACGCGGCCACGCCCACCGACCTGGAAAACGC
CAAGGCCCAGTTCCTGGGCAAGGCCGGGCGGGTGACCGAACTCCTCAAGGGCCTGGCCCAGCTGCCCGTCGAGGAGAAGA
AGTCCCGGGGTGCCGCGGTGAACGTGGCCAAGCAGGCCATCGAGCAGGCCCTGAACGAACGCCGCCAGGCCCTGGCCGAC
GCCGAACTGCAGGCGCAACTGCAGGCCGAGGCGCTCGACGTCACGCTGCCCGGCCGCCAGCGGGGGCAGGGCGGCCTGCA
TCCTGTCTCGCTTACCCTGGAGCGCATCGAGGGCATCTTCGGATCCATGGGTTTCGACGTGGCGGACGGCCCGGAAATCG
AATCCGACTGGTTCAACTTCACGGCATTGAACACGCCCGAGGACCATCCGGCGCGCTCGATGCACGACACCTTCTACGTG
GAGGGCGGCACCGCGTCCGCACCGCTGCTGCTGCGTACGCATACCAGCCCGATGCAGATCCGCCATGCCGTCCAGCACGT
GAAGAAGCACCGCGCGTTGCTGGATGCCGGCCAGCCCATGCCCGAGATCCGCGTGATCGCGCCGGGCCGTACCTACCGGG
TGGACAGCGACGCCACGCACTCGCCGATGTTCCACCAGTGCGAAGGGCTCTGGATCGGCGAGAACGTCAGCTTCAAGGAC
CTGAAGGTCGTGTTCACCGCCTTCTGCCGCACCTTCTTCGAAAGCGATGACCTGGTGCTGCGGTTCCGCCCGAGCTTCTT
CCCGTTCACCGAGCCGAGCGCCGAGATCGACATCCAGTTCCAGGACGGCCCGCTGGCAGGCCGCTGGCTGGAAGTGGCGG
GATCCGGGCAGGTGCATCCCAACGTGGTGCGCAACATGGGGCTCGATCCCGAGAAGTACATCGGCTTCGCGTTCGGCATG
GGCCCCGACCGGCTGACGATGCTGCGCTACGGCGTCAACGACCTGCGCCTGTTCTTCGACGGGGACATCCGCTTCCTCTC
GCAGTTCCAGTAA

Upstream 100 bases:

>100_bases
CTATCCTCCCCGGGTAGCCTGCCTGCGCAACAACAGCAAGGGCTAGGGCTTGAAAAGGCGCTAGCCCTTGTTTATTTCTC
CAAGACGACAAAGAGTTGGT

Downstream 100 bases:

>100_bases
TCGCCGAACCCCTTTTCGACTTCGCCGCGCGGGCCCGGCCCCCGCGGCGCCGGCCGTTCCTTTTGAACCGACTATGCAAT
TTCCTGAATCCTGGCTGCGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNELDSLVDAARRMFADAATPTDLENAKAQFLGKAGRVTELLKGLAQLPVEEKKSRGAAVNVAKQAIEQALNERRQALAD
AELQAQLQAEALDVTLPGRQRGQGGLHPVSLTLERIEGIFGSMGFDVADGPEIESDWFNFTALNTPEDHPARSMHDTFYV
EGGTASAPLLLRTHTSPMQIRHAVQHVKKHRALLDAGQPMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTAFCRTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQDGPLAGRWLEVAGSGQVHPNVVRNMGLDPEKYIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ

Sequences:

>Translated_350_residues
MNELDSLVDAARRMFADAATPTDLENAKAQFLGKAGRVTELLKGLAQLPVEEKKSRGAAVNVAKQAIEQALNERRQALAD
AELQAQLQAEALDVTLPGRQRGQGGLHPVSLTLERIEGIFGSMGFDVADGPEIESDWFNFTALNTPEDHPARSMHDTFYV
EGGTASAPLLLRTHTSPMQIRHAVQHVKKHRALLDAGQPMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTAFCRTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQDGPLAGRWLEVAGSGQVHPNVVRNMGLDPEKYIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
>Mature_350_residues
MNELDSLVDAARRMFADAATPTDLENAKAQFLGKAGRVTELLKGLAQLPVEEKKSRGAAVNVAKQAIEQALNERRQALAD
AELQAQLQAEALDVTLPGRQRGQGGLHPVSLTLERIEGIFGSMGFDVADGPEIESDWFNFTALNTPEDHPARSMHDTFYV
EGGTASAPLLLRTHTSPMQIRHAVQHVKKHRALLDAGQPMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTAFCRTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQDGPLAGRWLEVAGSGQVHPNVVRNMGLDPEKYIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=301, Percent_Identity=31.5614617940199, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=31.1111111111111, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1788007, Length=350, Percent_Identity=52.2857142857143, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32563657, Length=276, Percent_Identity=31.1594202898551, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=276, Percent_Identity=31.1594202898551, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=261, Percent_Identity=32.183908045977, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=373, Percent_Identity=26.5415549597855, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=93, Percent_Identity=39.7849462365591, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI18858079, Length=290, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137424, Length=302, Percent_Identity=27.4834437086093, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACIAC (A1TR35)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971197.1
- ProteinModelPortal:   A1TR35
- SMR:   A1TR35
- STRING:   A1TR35
- GeneID:   4668091
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2855
- NMPDR:   fig|397945.5.peg.2426
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   A1TR35
- ProtClustDB:   PRK00488
- BioCyc:   AAVE397945:AAVE_2855-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38913; Mature: 38913

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELDSLVDAARRMFADAATPTDLENAKAQFLGKAGRVTELLKGLAQLPVEEKKSRGAAV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCHHH
NVAKQAIEQALNERRQALADAELQAQLQAEALDVTLPGRQRGQGGLHPVSLTLERIEGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GSMGFDVADGPEIESDWFNFTALNTPEDHPARSMHDTFYVEGGTASAPLLLRTHTSPMQI
HCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHH
RHAVQHVKKHRALLDAGQPMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCHHHHCCCEEECCCCCHHH
LKVVFTAFCRTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQDGPLAGRWLEVAGSGQVHP
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCH
NVVRNMGLDPEKYIGFAFGMGPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
HHHHHCCCCHHHHEEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHCCC
>Mature Secondary Structure
MNELDSLVDAARRMFADAATPTDLENAKAQFLGKAGRVTELLKGLAQLPVEEKKSRGAAV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCHHH
NVAKQAIEQALNERRQALADAELQAQLQAEALDVTLPGRQRGQGGLHPVSLTLERIEGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GSMGFDVADGPEIESDWFNFTALNTPEDHPARSMHDTFYVEGGTASAPLLLRTHTSPMQI
HCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHH
RHAVQHVKKHRALLDAGQPMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCHHHHCCCEEECCCCCHHH
LKVVFTAFCRTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQDGPLAGRWLEVAGSGQVHP
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCH
NVVRNMGLDPEKYIGFAFGMGPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
HHHHHCCCCHHHHEEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA