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The map label for this gene is metR [H]

Identifier: 120611446

GI number: 120611446

Start: 3054854

End: 3055768

Strand: Direct

Name: metR [H]

Synonym: Aave_2782

Alternate gene names: 120611446

Gene position: 3054854-3055768 (Clockwise)

Preceding gene: 120611442

Following gene: 120611448

Centisome position: 57.07

GC content: 69.07

Gene sequence:

>915_bases
ATGCTGGAGCGCATCCACCTCAGCATCGTCCGGCAGGTCGAGAAGCAAGGCTCGCTGACGGCCGCCGCGGGCGTGCTGAA
CCTCACCCAGTCGGCCCTGAGCCACAGCATGAAGAAGCTCGAGCAGCAACTGGGCACCGATGTCTGGCTGCGCGAAGGCC
GCAGCCTGCGCCTGACGCAGGCGGGCCAGTACCTGCTGGCCGTGGCCAACCGCGTGCTGCCGCAACTGGAACTGGCCGAG
GAGCGGCTGGGCCAGTTCGCGCAGGGCGAGCGCGGCGCGCTGCGGATCGGCATGGAATGCCACCCGTGCTACCAATGGCT
GCTCAAGGTGGTTTCGCCCTACCTGACCGCGTGGCCCGACGTCGATGTGGACGTCAAGCAGAAGTTCCAGTTCGGCGGGA
TCGGCGCCCTCTTCGGCTACGAGATCGACCTGCTGGTCACGCCCGATCCGCTGTACAAGCCCGGGCTGCAGTTCGAGCCC
GTGTTCGACTACGAGCAGGTGCTGGTCGTGGCCGGGGGCCACGCCCTGGCTTCGGCGGCCTACGTGAAGCCGCAGCAGCT
GACCGGGGAAGTGCTCATCAGCTACCCGGTGGACATCGAGCGCCTGGACATCTACAACCAGTTCCTCCTGCCGGCCGGCG
TCACGCCCCGGCGCCACAAGGCCATCGAGACCACCGACATCATGTTGCAGATGGTGGCCAGCGGCCGCGGCGTGGCCGCC
CTGCCGCGGTGGCTGGTGGAGGAATACGCCGCCCGGATGGACGTGGTGCCCGTGCGGCTGGGTGCGCGCGGCATCGCCAA
GCAGATCTTCCTGGGCGCGCGCGAGGCGGACACCGCCATCGACTACGTGCGGGCCTTCATCGAGCTGGCGCGCCAGCCTG
CGGCCGCCACTGCGGGAAGAGCCTGGCGGCCATGA

Upstream 100 bases:

>100_bases
TTTCTTTCCTTCGTGTCAATGGTTCGGGCGGATGGTATGGGGCGGTATCAATGAAGTAAAATGGTTTTATTTCAACCATT
CATGAATACGACTCATTGAA

Downstream 100 bases:

>100_bases
GACGATGCTGAGGGGCGCGTGCGGGATCCCTATGATTGCGCCACGCCACCGGTAATGCAGGTCGTTGTCGGCCTTGCCGG
CGGTGACGCCGGCCCGCGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLERIHLSIVRQVEKQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLELAE
ERLGQFAQGERGALRIGMECHPCYQWLLKVVSPYLTAWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLQFEP
VFDYEQVLVVAGGHALASAAYVKPQQLTGEVLISYPVDIERLDIYNQFLLPAGVTPRRHKAIETTDIMLQMVASGRGVAA
LPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFIELARQPAAATAGRAWRP

Sequences:

>Translated_304_residues
MLERIHLSIVRQVEKQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLELAE
ERLGQFAQGERGALRIGMECHPCYQWLLKVVSPYLTAWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLQFEP
VFDYEQVLVVAGGHALASAAYVKPQQLTGEVLISYPVDIERLDIYNQFLLPAGVTPRRHKAIETTDIMLQMVASGRGVAA
LPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFIELARQPAAATAGRAWRP
>Mature_304_residues
MLERIHLSIVRQVEKQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLELAE
ERLGQFAQGERGALRIGMECHPCYQWLLKVVSPYLTAWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLQFEP
VFDYEQVLVVAGGHALASAAYVKPQQLTGEVLISYPVDIERLDIYNQFLLPAGVTPRRHKAIETTDIMLQMVASGRGVAA
LPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFIELARQPAAATAGRAWRP

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=295, Percent_Identity=40, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI157672245, Length=217, Percent_Identity=27.6497695852535, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI2367136, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=312, Percent_Identity=22.4358974358974, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787879, Length=264, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789204, Length=261, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=22.9787234042553, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=27.5280898876405, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=255, Percent_Identity=25.8823529411765, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=284, Percent_Identity=22.887323943662, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=109, Percent_Identity=34.8623853211009, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788296, Length=290, Percent_Identity=23.448275862069, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082024, Length=277, Percent_Identity=25.6317689530686, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33689; Mature: 33689

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERIHLSIVRQVEKQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEHH
AGQYLLAVANRVLPQLELAEERLGQFAQGERGALRIGMECHPCYQWLLKVVSPYLTAWPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
VDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLQFEPVFDYEQVLVVAGGHALASAA
CCCCHHHHHCCCCEEEEECEEEEEEECCCCCCCCCCCCCCCCCHHEEEEEECCHHHHHHH
YVKPQQLTGEVLISYPVDIERLDIYNQFLLPAGVTPRRHKAIETTDIMLQMVASGRGVAA
HCCHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHH
LPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFIELARQPAAATAGR
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCCC
AWRP
CCCC
>Mature Secondary Structure
MLERIHLSIVRQVEKQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEHH
AGQYLLAVANRVLPQLELAEERLGQFAQGERGALRIGMECHPCYQWLLKVVSPYLTAWPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
VDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLQFEPVFDYEQVLVVAGGHALASAA
CCCCHHHHHCCCCEEEEECEEEEEEECCCCCCCCCCCCCCCCCHHEEEEEECCHHHHHHH
YVKPQQLTGEVLISYPVDIERLDIYNQFLLPAGVTPRRHKAIETTDIMLQMVASGRGVAA
HCCHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHH
LPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFIELARQPAAATAGR
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCCC
AWRP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]