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The map label for this gene is nrtB [H]

Identifier: 120610821

GI number: 120610821

Start: 2356974

End: 2357921

Strand: Reverse

Name: nrtB [H]

Synonym: Aave_2146

Alternate gene names: 120610821

Gene position: 2357921-2356974 (Counterclockwise)

Preceding gene: 120610822

Following gene: 120610820

Centisome position: 44.05

GC content: 68.25

Gene sequence:

>948_bases
ATGGTCAGCGCCGTCTTCCATGCTCCCCCGGAGCCCGCCACCGCAGCCACCGCCGCGCATTCTGCCCCGGCCCCCTCCTC
CCCCGCATCCACGATGAAGACCGCCTCCCCGCCCGCCCCCGCAGCACCCGCCGCTGCCGTCCCGGCACCGCGCGACACCG
CCGCCGCCCTGCGCCGTTTCTGGCTCGCGGTGCTGCCGCCCGCCTGCGGCATCGGTCTGCTGCTGGTGGTTTGGGCCATC
GTCTCCACCACCACGGGCGGAAGCATTCCCAACCCGTCCGACACCTGGAAGCAGGCCGTGGAGATCTTCAGCGACCCGTT
CTACCGCAACGGCCCCAACGACCAGGGCGTGGGCTGGAACGTGCTGGCCTCGCTCGAGCGCGTGGCCGTCGGCTTCGGCC
TCGCCGCCGTGGTGGGCATCCCGGCCGGCTTCGTGATCGGGCGCTTCACCTTCCTCTCGCGCATGTTCAACCCGCTCATC
AGCCTGCTGCGCCCGGTCTCACCGCTGGCCTGGCTGCCGATCGGCCTGCTGGTCTTCAAGGGCGCGAACCCCGCGGCCAT
CTGGACCATCTTCATCTGCTCCATCTGGCCGATGATCATCAACACCGCCGTGGGCGTGCAGCGCGTGCCGCAGGACTACA
TGAACGTGGCCCGCGTGCTGAATCTCTCCGAATGGAAGATCGCCACCACCATCCTCTTCCCCTCGGTGCTGCCCTACATG
CTCACCGGCGTGCGCCTGGCCGTCGGCACCGCGTGGCTGGTGATCGTCGCTGCCGAAATGCTGACCGGCGGCGTGGGCAT
CGGCTTCTGGGTGTGGGACGAGTGGAACAACCTGAACGTCAAGAACATCCTGATCGCGATCTTCGTGATCGGCATCGTCG
GCCTGCTGCTCGAATACGCCCTCGTCAAGCTCGCCACGGCCTTCTCGTTCGAAGAGGTCAAGGCCTGA

Upstream 100 bases:

>100_bases
CATCGTCTGGGACGGCAAGGACCCCGCGAAATACGCGGACGGTTTCAAGATCAAGGCCTGAGCGCCGGCCCCCACCCCGA
TCACCGCGAAGGAGTCCACC

Downstream 100 bases:

>100_bases
TCCGGTCCGACCCGATCCACCCCACACCGGAGTTCCCCATGCATGCCTCCCTCAACACCAAGTTCATCGAAATCCAGGGC
GTGGAGCAGACCTTCAAGAC

Product: nitrate ABC transporter inner membrane subunit

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MVSAVFHAPPEPATAATAAHSAPAPSSPASTMKTASPPAPAAPAAAVPAPRDTAAALRRFWLAVLPPACGIGLLLVVWAI
VSTTTGGSIPNPSDTWKQAVEIFSDPFYRNGPNDQGVGWNVLASLERVAVGFGLAAVVGIPAGFVIGRFTFLSRMFNPLI
SLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKIATTILFPSVLPYM
LTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNILIAIFVIGIVGLLLEYALVKLATAFSFEEVKA

Sequences:

>Translated_315_residues
MVSAVFHAPPEPATAATAAHSAPAPSSPASTMKTASPPAPAAPAAAVPAPRDTAAALRRFWLAVLPPACGIGLLLVVWAI
VSTTTGGSIPNPSDTWKQAVEIFSDPFYRNGPNDQGVGWNVLASLERVAVGFGLAAVVGIPAGFVIGRFTFLSRMFNPLI
SLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKIATTILFPSVLPYM
LTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNILIAIFVIGIVGLLLEYALVKLATAFSFEEVKA
>Mature_315_residues
MVSAVFHAPPEPATAATAAHSAPAPSSPASTMKTASPPAPAAPAAAVPAPRDTAAALRRFWLAVLPPACGIGLLLVVWAI
VSTTTGGSIPNPSDTWKQAVEIFSDPFYRNGPNDQGVGWNVLASLERVAVGFGLAAVVGIPAGFVIGRFTFLSRMFNPLI
SLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKIATTILFPSVLPYM
LTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNILIAIFVIGIVGLLLEYALVKLATAFSFEEVKA

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=242, Percent_Identity=30.1652892561983, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081802, Length=167, Percent_Identity=26.9461077844311, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33406; Mature: 33406

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAVFHAPPEPATAATAAHSAPAPSSPASTMKTASPPAPAAPAAAVPAPRDTAAALRRF
CCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
WLAVLPPACGIGLLLVVWAIVSTTTGGSIPNPSDTWKQAVEIFSDPFYRNGPNDQGVGWN
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VLASLERVAVGFGLAAVVGIPAGFVIGRFTFLSRMFNPLISLLRPVSPLAWLPIGLLVFK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKIATTILFPSVLPYM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNILIAIFVIGIVGLLLEYA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVKLATAFSFEEVKA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSAVFHAPPEPATAATAAHSAPAPSSPASTMKTASPPAPAAPAAAVPAPRDTAAALRRF
CCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
WLAVLPPACGIGLLLVVWAIVSTTTGGSIPNPSDTWKQAVEIFSDPFYRNGPNDQGVGWN
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VLASLERVAVGFGLAAVVGIPAGFVIGRFTFLSRMFNPLISLLRPVSPLAWLPIGLLVFK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKIATTILFPSVLPYM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNILIAIFVIGIVGLLLEYA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVKLATAFSFEEVKA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]