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The map label for this gene is mexA [H]

Identifier: 120610789

GI number: 120610789

Start: 2320676

End: 2322028

Strand: Reverse

Name: mexA [H]

Synonym: Aave_2112

Alternate gene names: 120610789

Gene position: 2322028-2320676 (Counterclockwise)

Preceding gene: 120610792

Following gene: 120610788

Centisome position: 43.38

GC content: 74.58

Gene sequence:

>1353_bases
ATGAATTCCACGCCCTCCTGCTCCGCCCCCCGCTTCGCCGTCCCTCCCGTTTCCGCCGCTGCCTTTCCGCTCGAACGACC
GTCGCGCACTCCCACGGTCGCGCTGGAGGGTGGCCGCGCCCGTGGCGCACGGCGGATCCGCGCCGCGTGGCTGGTGCTGG
GCCTGGGGACGATCCTGGCCGGCTGTTCGGACAAGGCCCCGGCGCCGGCGCCGCGCGGCCCCGTGGAAGTGGGCGTGGTC
ACGCTGCAGCCCGAGCGCCAGTCCGTGACCACCGAATTGCCGGGACGCACCAATGCCTATCTCGTGGCCGAGATCCGGCC
CCAGGTGGGCGGGATCGTGCAGAAACGACTGTTCACGGAGGGCGCGGAAGTGAAGGCGGGCCAGGCGCTCTACCAGCTCG
ACCCGGCGCCGCTGGAGGCGGCCCTGGCCAGCGCCGAGGCCTCGCTGGGCCGGGCCCAGGCCACCGCCGCATCGGCGGAG
TCGAACGCGCGGCGCAATGCCGAGCTGGCGAAGATCGAGGCCGTCAGCCGGCAGGTGGCGGACGACAGCCAGGCGGCGGC
CCAGCAGGCGCGCTCGGATGTGGCCGTGGCACGCGCCGCGGTGGCCACGGCGCGCATCAACCTGGGTTACGCGCGCATCC
AGTCGCCCATCTCCGGCCGCACGGCGACGTCCAGCGTCACGCCCGGGGCCCTGGTCACCGCCAACCAGGCGACCGCCCTC
ACCACCGTCTCGCAGGTCGATCCGCTCTACGTGGACGTCACCCAGTCGAGCACCGACGTGCTGCGCTGGAAGACCGAACT
GGCGAATGGCCGGCTGCAGCGCGTCGGGGAGGGCCAGGCGCGCATGCGGCTCAGGCTCGAGGACGGCAGCACCTATGCCC
ACGCCGGCCGGCTGCAGTTCAGCGGCATCACGGTCAATCCGACGACCGGCGCGGTCACGCTGCGCGGCGTGGTGCCCAAC
CCGGACGGGCTGCTCATGCCCGGCATGTACGTGCGCGCCGTGCTGGAGACCGGCGTGAACGAGCAGGCGCTGCTCGTGCC
GCAGCAGGGCGTGACGCGCGACGCCGCCGGCAATGCCAGCGTGCTGGTCGCCAACGCGCAGGACCAGGTGGAGCGCCGCG
GCATCGAGGTGGGCGCGGCCGTGGGCAATCGCTGGGTGGCGACGGCGGGCATGGCGCCGGGCGACCGCGTCATCGTGGAC
GGCCTGCAGCGCATCCAGCCGGGCGACGCGGTGAAGCCGGTGGAGGTGAAGAGCCTGCCGCAGGGCGCCGCGCTCTCGCC
CTCCCAGGGAACATCGGCGCCGGCACCCGCTGCCCCCGGCGCCGCGGGCAGCGCCCCGGCGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCGGCCGCAGGAACGGAATGCGGAAACGCTGGCCTTCGCGCTTCACAAGCCGTTTTCATTCGGCGGTGAGAATGCCTGG
TTCTCCCAGCAGCGCCCGCC

Downstream 100 bases:

>100_bases
GGACGCGCCATGGCCCAATTCTTCATCCACCGGCCCATCTTCGCGTGGGTCATCGCCATCGTCATCATGCTCGCGGGCGC
GATGTCGATCCGCAATCTGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNSTPSCSAPRFAVPPVSAAAFPLERPSRTPTVALEGGRARGARRIRAAWLVLGLGTILAGCSDKAPAPAPRGPVEVGVV
TLQPERQSVTTELPGRTNAYLVAEIRPQVGGIVQKRLFTEGAEVKAGQALYQLDPAPLEAALASAEASLGRAQATAASAE
SNARRNAELAKIEAVSRQVADDSQAAAQQARSDVAVARAAVATARINLGYARIQSPISGRTATSSVTPGALVTANQATAL
TTVSQVDPLYVDVTQSSTDVLRWKTELANGRLQRVGEGQARMRLRLEDGSTYAHAGRLQFSGITVNPTTGAVTLRGVVPN
PDGLLMPGMYVRAVLETGVNEQALLVPQQGVTRDAAGNASVLVANAQDQVERRGIEVGAAVGNRWVATAGMAPGDRVIVD
GLQRIQPGDAVKPVEVKSLPQGAALSPSQGTSAPAPAAPGAAGSAPAAAR

Sequences:

>Translated_450_residues
MNSTPSCSAPRFAVPPVSAAAFPLERPSRTPTVALEGGRARGARRIRAAWLVLGLGTILAGCSDKAPAPAPRGPVEVGVV
TLQPERQSVTTELPGRTNAYLVAEIRPQVGGIVQKRLFTEGAEVKAGQALYQLDPAPLEAALASAEASLGRAQATAASAE
SNARRNAELAKIEAVSRQVADDSQAAAQQARSDVAVARAAVATARINLGYARIQSPISGRTATSSVTPGALVTANQATAL
TTVSQVDPLYVDVTQSSTDVLRWKTELANGRLQRVGEGQARMRLRLEDGSTYAHAGRLQFSGITVNPTTGAVTLRGVVPN
PDGLLMPGMYVRAVLETGVNEQALLVPQQGVTRDAAGNASVLVANAQDQVERRGIEVGAAVGNRWVATAGMAPGDRVIVD
GLQRIQPGDAVKPVEVKSLPQGAALSPSQGTSAPAPAAPGAAGSAPAAAR
>Mature_450_residues
MNSTPSCSAPRFAVPPVSAAAFPLERPSRTPTVALEGGRARGARRIRAAWLVLGLGTILAGCSDKAPAPAPRGPVEVGVV
TLQPERQSVTTELPGRTNAYLVAEIRPQVGGIVQKRLFTEGAEVKAGQALYQLDPAPLEAALASAEASLGRAQATAASAE
SNARRNAELAKIEAVSRQVADDSQAAAQQARSDVAVARAAVATARINLGYARIQSPISGRTATSSVTPGALVTANQATAL
TTVSQVDPLYVDVTQSSTDVLRWKTELANGRLQRVGEGQARMRLRLEDGSTYAHAGRLQFSGITVNPTTGAVTLRGVVPN
PDGLLMPGMYVRAVLETGVNEQALLVPQQGVTRDAAGNASVLVANAQDQVERRGIEVGAAVGNRWVATAGMAPGDRVIVD
GLQRIQPGDAVKPVEVKSLPQGAALSPSQGTSAPAPAAPGAAGSAPAAAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=365, Percent_Identity=50.958904109589, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=50.9485094850949, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=45.9677419354839, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87082036, Length=337, Percent_Identity=27.0029673590504, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46209; Mature: 46209

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTPSCSAPRFAVPPVSAAAFPLERPSRTPTVALEGGRARGARRIRAAWLVLGLGTILA
CCCCCCCCCCCEECCCCCHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GCSDKAPAPAPRGPVEVGVVTLQPERQSVTTELPGRTNAYLVAEIRPQVGGIVQKRLFTE
CCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHCC
GAEVKAGQALYQLDPAPLEAALASAEASLGRAQATAASAESNARRNAELAKIEAVSRQVA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHCHHCCCHHHHHHHHHHHHC
DDSQAAAQQARSDVAVARAAVATARINLGYARIQSPISGRTATSSVTPGALVTANQATAL
CCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCEE
TTVSQVDPLYVDVTQSSTDVLRWKTELANGRLQRVGEGQARMRLRLEDGSTYAHAGRLQF
EEHHHCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCCCCEEEEEEEECCCEEEECCEEEE
SGITVNPTTGAVTLRGVVPNPDGLLMPGMYVRAVLETGVNEQALLVPQQGVTRDAAGNAS
CCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCE
VLVANAQDQVERRGIEVGAAVGNRWVATAGMAPGDRVIVDGLQRIQPGDAVKPVEVKSLP
EEEECCHHHHHHCCCEEEHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
QGAALSPSQGTSAPAPAAPGAAGSAPAAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSTPSCSAPRFAVPPVSAAAFPLERPSRTPTVALEGGRARGARRIRAAWLVLGLGTILA
CCCCCCCCCCCEECCCCCHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GCSDKAPAPAPRGPVEVGVVTLQPERQSVTTELPGRTNAYLVAEIRPQVGGIVQKRLFTE
CCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHCC
GAEVKAGQALYQLDPAPLEAALASAEASLGRAQATAASAESNARRNAELAKIEAVSRQVA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHCHHCCCHHHHHHHHHHHHC
DDSQAAAQQARSDVAVARAAVATARINLGYARIQSPISGRTATSSVTPGALVTANQATAL
CCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCEE
TTVSQVDPLYVDVTQSSTDVLRWKTELANGRLQRVGEGQARMRLRLEDGSTYAHAGRLQF
EEHHHCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCCCCEEEEEEEECCCEEEECCEEEE
SGITVNPTTGAVTLRGVVPNPDGLLMPGMYVRAVLETGVNEQALLVPQQGVTRDAAGNAS
CCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCE
VLVANAQDQVERRGIEVGAAVGNRWVATAGMAPGDRVIVDGLQRIQPGDAVKPVEVKSLP
EEEECCHHHHHHCCCEEEHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
QGAALSPSQGTSAPAPAAPGAAGSAPAAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]