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The map label for this gene is garD [H]

Identifier: 120610760

GI number: 120610760

Start: 2284415

End: 2285944

Strand: Reverse

Name: garD [H]

Synonym: Aave_2082

Alternate gene names: 120610760

Gene position: 2285944-2284415 (Counterclockwise)

Preceding gene: 120610761

Following gene: 120610759

Centisome position: 42.71

GC content: 68.95

Gene sequence:

>1530_bases
ATGCCCAGCCTTGACAGTCCGCTGCTGCACCTGCATCCCAATGACAACGTGTTCGTCGCCAAGGCGGCGCTGGCGCTGGG
GCAGGAGATTCCCGAACTGGGGGTGCGGGTACGCGCGCAGGTGCCGCCGGGGCACAAGATCGCAGCGCGGCGCATCGCGC
GGGGCGAGCAGGTGAAGAAGTACGACACGGTGATCGGCGTGGCGACGCGCGACCTGGAGCCGGGCGAGTACGTGCACAGC
CACAACCTGGAGATCGTGGACCATTACCGCGACCCGGCGTTCTGCGCGGATGTCCGGCCGGTCCATTACGTGCCCGAGGC
CGAGCGCGCGACCTTCCAGGGTTTCGTGCGCGCGGATGGCTCCGTGGGCACGCGTAACTTCATCGGCATCCTGTCGTCGG
TCAATTGCTCCGCCACGGTCATCAAGCAGATCGCCGCGCATTTCACGCAGGAGCGGATGCGCGCATTCCCCCACGTGGAT
GGCGTGGTGGCGTTCGCGCAGACCAGCGGCTGCGGCATGTCCTCGCCCAGCGAGCATTTCGACGTGCTGCGCCGCACGAT
CGCGGGCTATGCCCGGCACCCGAACCTGGCCGGCGTGCTGATCGTGGGCCTGGGCTGCGAGCGCAACCAGGTGGACGGGC
TGGTCGAATCCCAGGGCCTGGAGCGCGGCCGGCTGATGCGCACGCTGGTGATGCAGGACGTGGGCGGCACGCGGGCGACC
ATCGCTGCCGGCATCGCGGCGATCGAGGAGATGCTGCCGGCCGCCAACGAGGCGCGCCGCAGCACGGTGCCCGCCAGCCA
CCTGAAGATCGGGCTCGAATGCGGCGGATCGGACGGCTTCTCCGGCATCACGGCCAACCCGGCGCTGGGCGCGGCGATGG
ACGTGCTCGTGCGCCATGGCGGCACCGCCATCCTGTCGGAAACGCCGGAAATCCACGGCGTGGAGTTCATGCTCACGCGC
CGCGCCATCAGCCCCGCTGTGGGCCAGAAGCTGCTGGACCGCCTGGCCTGGTGGGAGCGCTACGCGGCCGGGCAGAACGC
GCAGTTCAACGGCGTGGTGGGCCACGGCAACCAGGCCGGCGGCCTGGCGAACATCTTCGAGAAATCGCTGGGCAGCGCGA
TGAAAGGTGGCACCACGCCGCTGCGCGCCGTGTACGAATACGCCGAGCGCATCGACGAGAGCGGTTTCGTGTTCATGGAT
TCGCCGGGTTACGACCCGGTCGCCGCCACGGGACAGATCGCGAGCGGCGCGCAGCTGATCTGCTTCACCACCGGGCGCGG
CTCGATGTTCGGCAGCAAGCCGGCACCGACCATCAAGCTGGCCAGCAACACGCCCATGTACCGGCGCCTGGAGGAGGACA
TGGACATCAACTGCGGCGTGATCCTGGACGGCGAACTGACGGTGCCCGAGATGGGCGAGCGCATCTTCGCGCAGATCCTG
CGGCACGCGAGCGGCGAGGCGACCAAGAGCGAGCTGCTGGGCCTGGGCGACCACGAGTTCGTGCCCTGGCACCTGGGTAT
CGTGAGCTGA

Upstream 100 bases:

>100_bases
CGGCCGCGCCGGCGTCCGCGGCCTCGCCGCTGCGCGAGGTCCGGGCGAGGCATGCCCACACATCCCACGCACACGCCCTA
CCCCATGATGAAAGCCCCGC

Downstream 100 bases:

>100_bases
GCCCCGGTTCCGAGGCGGTGCGGTGCGGTTCTGCCCGACGTTCCGGCCCGCACGCGCTGCCATGCAGGCACCAGCGCGAC
ACCCGGCCTTTACCGCCGGA

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPSLDSPLLHLHPNDNVFVAKAALALGQEIPELGVRVRAQVPPGHKIAARRIARGEQVKKYDTVIGVATRDLEPGEYVHS
HNLEIVDHYRDPAFCADVRPVHYVPEAERATFQGFVRADGSVGTRNFIGILSSVNCSATVIKQIAAHFTQERMRAFPHVD
GVVAFAQTSGCGMSSPSEHFDVLRRTIAGYARHPNLAGVLIVGLGCERNQVDGLVESQGLERGRLMRTLVMQDVGGTRAT
IAAGIAAIEEMLPAANEARRSTVPASHLKIGLECGGSDGFSGITANPALGAAMDVLVRHGGTAILSETPEIHGVEFMLTR
RAISPAVGQKLLDRLAWWERYAAGQNAQFNGVVGHGNQAGGLANIFEKSLGSAMKGGTTPLRAVYEYAERIDESGFVFMD
SPGYDPVAATGQIASGAQLICFTTGRGSMFGSKPAPTIKLASNTPMYRRLEEDMDINCGVILDGELTVPEMGERIFAQIL
RHASGEATKSELLGLGDHEFVPWHLGIVS

Sequences:

>Translated_509_residues
MPSLDSPLLHLHPNDNVFVAKAALALGQEIPELGVRVRAQVPPGHKIAARRIARGEQVKKYDTVIGVATRDLEPGEYVHS
HNLEIVDHYRDPAFCADVRPVHYVPEAERATFQGFVRADGSVGTRNFIGILSSVNCSATVIKQIAAHFTQERMRAFPHVD
GVVAFAQTSGCGMSSPSEHFDVLRRTIAGYARHPNLAGVLIVGLGCERNQVDGLVESQGLERGRLMRTLVMQDVGGTRAT
IAAGIAAIEEMLPAANEARRSTVPASHLKIGLECGGSDGFSGITANPALGAAMDVLVRHGGTAILSETPEIHGVEFMLTR
RAISPAVGQKLLDRLAWWERYAAGQNAQFNGVVGHGNQAGGLANIFEKSLGSAMKGGTTPLRAVYEYAERIDESGFVFMD
SPGYDPVAATGQIASGAQLICFTTGRGSMFGSKPAPTIKLASNTPMYRRLEEDMDINCGVILDGELTVPEMGERIFAQIL
RHASGEATKSELLGLGDHEFVPWHLGIVS
>Mature_508_residues
PSLDSPLLHLHPNDNVFVAKAALALGQEIPELGVRVRAQVPPGHKIAARRIARGEQVKKYDTVIGVATRDLEPGEYVHSH
NLEIVDHYRDPAFCADVRPVHYVPEAERATFQGFVRADGSVGTRNFIGILSSVNCSATVIKQIAAHFTQERMRAFPHVDG
VVAFAQTSGCGMSSPSEHFDVLRRTIAGYARHPNLAGVLIVGLGCERNQVDGLVESQGLERGRLMRTLVMQDVGGTRATI
AAGIAAIEEMLPAANEARRSTVPASHLKIGLECGGSDGFSGITANPALGAAMDVLVRHGGTAILSETPEIHGVEFMLTRR
AISPAVGQKLLDRLAWWERYAAGQNAQFNGVVGHGNQAGGLANIFEKSLGSAMKGGTTPLRAVYEYAERIDESGFVFMDS
PGYDPVAATGQIASGAQLICFTTGRGSMFGSKPAPTIKLASNTPMYRRLEEDMDINCGVILDGELTVPEMGERIFAQILR
HASGEATKSELLGLGDHEFVPWHLGIVS

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=510, Percent_Identity=34.7058823529412, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1789475, Length=494, Percent_Identity=34.6153846153846, Blast_Score=254, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 54530; Mature: 54399

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLDSPLLHLHPNDNVFVAKAALALGQEIPELGVRVRAQVPPGHKIAARRIARGEQVKK
CCCCCCCEEEECCCCCEEEHHHHHHHHHHCHHCCCEEEEECCCCHHHHHHHHHCCHHHHH
YDTVIGVATRDLEPGEYVHSHNLEIVDHYRDPAFCADVRPVHYVPEAERATFQGFVRADG
HHHEEEEEECCCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHEECCC
SVGTRNFIGILSSVNCSATVIKQIAAHFTQERMRAFPHVDGVVAFAQTSGCGMSSPSEHF
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
DVLRRTIAGYARHPNLAGVLIVGLGCERNQVDGLVESQGLERGRLMRTLVMQDVGGTRAT
HHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHHHHCCCHHHHHHHHHHHHHCCCCHHH
IAAGIAAIEEMLPAANEARRSTVPASHLKIGLECGGSDGFSGITANPALGAAMDVLVRHG
HHHHHHHHHHHHCCHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GTAILSETPEIHGVEFMLTRRAISPAVGQKLLDRLAWWERYAAGQNAQFNGVVGHGNQAG
CCEEECCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCEEECCCCCC
GLANIFEKSLGSAMKGGTTPLRAVYEYAERIDESGFVFMDSPGYDPVAATGQIASGAQLI
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFTTGRGSMFGSKPAPTIKLASNTPMYRRLEEDMDINCGVILDGELTVPEMGERIFAQIL
EEECCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHH
RHASGEATKSELLGLGDHEFVPWHLGIVS
HHCCCCCCHHHHHCCCCCCCCCEEEEECC
>Mature Secondary Structure 
PSLDSPLLHLHPNDNVFVAKAALALGQEIPELGVRVRAQVPPGHKIAARRIARGEQVKK
CCCCCCEEEECCCCCEEEHHHHHHHHHHCHHCCCEEEEECCCCHHHHHHHHHCCHHHHH
YDTVIGVATRDLEPGEYVHSHNLEIVDHYRDPAFCADVRPVHYVPEAERATFQGFVRADG
HHHEEEEEECCCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHEECCC
SVGTRNFIGILSSVNCSATVIKQIAAHFTQERMRAFPHVDGVVAFAQTSGCGMSSPSEHF
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
DVLRRTIAGYARHPNLAGVLIVGLGCERNQVDGLVESQGLERGRLMRTLVMQDVGGTRAT
HHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHHHHCCCHHHHHHHHHHHHHCCCCHHH
IAAGIAAIEEMLPAANEARRSTVPASHLKIGLECGGSDGFSGITANPALGAAMDVLVRHG
HHHHHHHHHHHHCCHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GTAILSETPEIHGVEFMLTRRAISPAVGQKLLDRLAWWERYAAGQNAQFNGVVGHGNQAG
CCEEECCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCEEECCCCCC
GLANIFEKSLGSAMKGGTTPLRAVYEYAERIDESGFVFMDSPGYDPVAATGQIASGAQLI
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFTTGRGSMFGSKPAPTIKLASNTPMYRRLEEDMDINCGVILDGELTVPEMGERIFAQIL
EEECCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHH
RHASGEATKSELLGLGDHEFVPWHLGIVS
HHCCCCCCHHHHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]