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The map label for this gene is hemX [C]

Identifier: 120610677

GI number: 120610677

Start: 2169435

End: 2170517

Strand: Reverse

Name: hemX [C]

Synonym: Aave_1997

Alternate gene names: 120610677

Gene position: 2170517-2169435 (Counterclockwise)

Preceding gene: 120610678

Following gene: 120610676

Centisome position: 40.55

GC content: 74.15

Gene sequence:

>1083_bases
ATGAGTTCCGAGCCCACCCACGCACCTTCCGCGCCTCCCGCAGGCCCGGCGCCCCAGGCCTCCGGCGTGCAGCGCGCCGT
GCTGACGCTGCTCGGCGTCGTGGCCGTCGCAGGCCTCGCGACCAGCGTCATGCTGTGGCAGCGCCTCGGCAGCATCCAGG
AACAGCTCGCGCGCCAGTCGGCCGATGCCGGCGCGCAGTCCATCGAGGCCCGCACCCTCGCCAACCAGGCGCTGGACATG
GCGCGCGACGTCTCGGCGCGCATCGCCGTGAACGAAACCCGCGTGAGCGAAGTCGCCCTGCAACGCAGCCAGCTCGAGGA
ACTCATGCAGAGCCTCTCGCGCTCGCGCGACGAGAACCTGGTGGTGGACATCGAATCCGCGCTGCGCCTCGCGCAGCAGC
AGGCCCAGCTCACCGGCAGCCTGGAGCCCGTCATGGCCGCGCTCAAGAGCGCCAGCCAGCGCATCGAGCGGGCCGCGCAG
CCGCGCCTGGCCCCCGTGGCGCGCGCCATCGGCCGCGACCTCGACCGCGTGGGCTCGGCGCAGGTCACCGACACGGCCGG
CCTGCTGGCCCGCCTGGACGACCTCATGCGCCAGGTGGACGAACTGCCCCTGCAGAACGCCGTGGCCCAGGCCGCGGCCA
CGCGGCGCATGAATGCCGCCGCCCGCCCGTCCGAGGGCCCGGCCGCGCCCGGAGCCGACGGCGCCCTGCCCTGGTGGCAA
GCCGCGCTGCAGCGCGGCTGGGAAGTCGTGCGCGACGAGGCCCGCCAGCTGCTGCGCGTCACCCGCATCGACCGCCCGGA
AGCCATCCTCATCGCCCCCGATCAGGCCTTCTTCCTGCGCGAGAACCTCAAGCTCCAGCTGATGAACGCCCGCCTCGCGC
TGCTGGCGCGCCAGTACGAATCGGCGCGCGCAGACCTCTCCGCCGCCAACAACGCCCTGGGCCGGTACTTCGATCCCGCA
TCGCGCCGCACGCAGACAGCGGCCACGGTGCTGCAGCAGGCGCAGGTCCACCTCAAGGGCGCCGCCCTGCCCACGCTGGA
CGAAACCTTCGCCGCGCTGGCCACCGCCGCCGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
ATCCCCGCATCGCGCAGGCCGCGCAGGACGCCGGATTCGGGGCCGTGCGCCAGTGCCGCCCGGCCCTGAACGACGTGGTC
GCATCGATAGAATCCCACCC

Downstream 100 bases:

>100_bases
CCCGCGCAGGAGACCACCCACCCATGCGCGCAGCACTCTGGTTCCTGGTACTTTTCGGCACGGCCGTGGCCGCCGCCCTG
TTCGCGGGCAACAACCAGGG

Product: hypothetical protein

Products: S-adenosyl-L-homocysteine; precorrin-1; Precorrin 2

Alternate protein names: HemX Family Protein; Porphobilinogen Deaminase; Uroporphyrin-III C-Methyltransferase HemX; Uroporphyrin-III Methylase; Of Heme Biosynthesis

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSSEPTHAPSAPPAGPAPQASGVQRAVLTLLGVVAVAGLATSVMLWQRLGSIQEQLARQSADAGAQSIEARTLANQALDM
ARDVSARIAVNETRVSEVALQRSQLEELMQSLSRSRDENLVVDIESALRLAQQQAQLTGSLEPVMAALKSASQRIERAAQ
PRLAPVARAIGRDLDRVGSAQVTDTAGLLARLDDLMRQVDELPLQNAVAQAAATRRMNAAARPSEGPAAPGADGALPWWQ
AALQRGWEVVRDEARQLLRVTRIDRPEAILIAPDQAFFLRENLKLQLMNARLALLARQYESARADLSAANNALGRYFDPA
SRRTQTAATVLQQAQVHLKGAALPTLDETFAALATAAAGR

Sequences:

>Translated_360_residues
MSSEPTHAPSAPPAGPAPQASGVQRAVLTLLGVVAVAGLATSVMLWQRLGSIQEQLARQSADAGAQSIEARTLANQALDM
ARDVSARIAVNETRVSEVALQRSQLEELMQSLSRSRDENLVVDIESALRLAQQQAQLTGSLEPVMAALKSASQRIERAAQ
PRLAPVARAIGRDLDRVGSAQVTDTAGLLARLDDLMRQVDELPLQNAVAQAAATRRMNAAARPSEGPAAPGADGALPWWQ
AALQRGWEVVRDEARQLLRVTRIDRPEAILIAPDQAFFLRENLKLQLMNARLALLARQYESARADLSAANNALGRYFDPA
SRRTQTAATVLQQAQVHLKGAALPTLDETFAALATAAAGR
>Mature_359_residues
SSEPTHAPSAPPAGPAPQASGVQRAVLTLLGVVAVAGLATSVMLWQRLGSIQEQLARQSADAGAQSIEARTLANQALDMA
RDVSARIAVNETRVSEVALQRSQLEELMQSLSRSRDENLVVDIESALRLAQQQAQLTGSLEPVMAALKSASQRIERAAQP
RLAPVARAIGRDLDRVGSAQVTDTAGLLARLDDLMRQVDELPLQNAVAQAAATRRMNAAARPSEGPAAPGADGALPWWQA
ALQRGWEVVRDEARQLLRVTRIDRPEAILIAPDQAFFLRENLKLQLMNARLALLARQYESARADLSAANNALGRYFDPAS
RRTQTAATVLQQAQVHLKGAALPTLDETFAALATAAAGR

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.107

Molecular weight: Translated: 38493; Mature: 38362

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEPTHAPSAPPAGPAPQASGVQRAVLTLLGVVAVAGLATSVMLWQRLGSIQEQLARQS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAGAQSIEARTLANQALDMARDVSARIAVNETRVSEVALQRSQLEELMQSLSRSRDENL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVDIESALRLAQQQAQLTGSLEPVMAALKSASQRIERAAQPRLAPVARAIGRDLDRVGSA
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
QVTDTAGLLARLDDLMRQVDELPLQNAVAQAAATRRMNAAARPSEGPAAPGADGALPWWQ
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AALQRGWEVVRDEARQLLRVTRIDRPEAILIAPDQAFFLRENLKLQLMNARLALLARQYE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
SARADLSAANNALGRYFDPASRRTQTAATVLQQAQVHLKGAALPTLDETFAALATAAAGR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSEPTHAPSAPPAGPAPQASGVQRAVLTLLGVVAVAGLATSVMLWQRLGSIQEQLARQS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAGAQSIEARTLANQALDMARDVSARIAVNETRVSEVALQRSQLEELMQSLSRSRDENL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVDIESALRLAQQQAQLTGSLEPVMAALKSASQRIERAAQPRLAPVARAIGRDLDRVGSA
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
QVTDTAGLLARLDDLMRQVDELPLQNAVAQAAATRRMNAAARPSEGPAAPGADGALPWWQ
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AALQRGWEVVRDEARQLLRVTRIDRPEAILIAPDQAFFLRENLKLQLMNARLALLARQYE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
SARADLSAANNALGRYFDPASRRTQTAATVLQQAQVHLKGAALPTLDETFAALATAAAGR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; uroporphyrin III; precorrin-1

Specific reaction: (1) S-adenosyl-L-methionine + uroporphyrin III = S-adenosyl-L-homocysteine + precorrin-1; (2) S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA