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The map label for this gene is gcvA [H]

Identifier: 120610663

GI number: 120610663

Start: 2152310

End: 2153209

Strand: Direct

Name: gcvA [H]

Synonym: Aave_1983

Alternate gene names: 120610663

Gene position: 2152310-2153209 (Clockwise)

Preceding gene: 120610658

Following gene: 120610664

Centisome position: 40.21

GC content: 72.44

Gene sequence:

>900_bases
ATGCGCCGCACCCTGCCGTCCACCCAGGCACTGGCCTGTTTCGAAGCCGCCGCCCGCCATGAAAGCTACACGCGGGCGGC
CCAGGAACTGTTCCTCACGCAAAGCGCCGTGTCGCGGCAGATCATGGCGCTGGAGGAGTTCCTGGGCGTGGCGCTGTTCC
GCCGCACCCGCCACGGCGTGGCGCTGACGCCGGCCGGCACGCACTACGCCGCCCAGGTCGGGCGCTGGCTCGAAGGCCTG
GAGCGCGACACGCTCGACGTCATGGCCCACCAGGGGCAGGGCGGTTCCATCCGCCTCGCCGCCGTGCCCACCTTCGCCAC
CCGCTGGCTGGTGCCCCGCCTGCCCCGGCTGGCCGAGCGGCATCCCGACATCGTCGTTCACATCGAGACCCAGGTGCGGC
CCTTCCTTTTCGCGGATGCGGGCTTCGATGCGGCCCTTTATGCCGGCACGCCCGAGCAGGTGGCCAATTGGCCCGGCACC
CAGGCCCTGCGGTTGATGCAGGAGGATGTGGCTCCGGTCTGCAGCCCCGCGCTGCTGCCGGAGGGGCGGCCCCTGCCGCC
GGAAGCCCTGGCGGAACTGCCGCTGCTGCAGCAGACGACGCGGCCGCTGGCCTGGCGCCAGTGGTTCGAATCCATGGGGG
TGCATGCCCCACAGCATGCCCTGGACGGCCCGCGCTACGAGCTCTTTTCCATGTCCGCCGTGGCCGCCGCCCACGGCCTG
GGCGTGGCGCTGGTACCGCTGATGCTGATCGAGCCCGAGCGTGCCCGGGGCGAACTGGTGCCGGCCTGCGACCGCGCGCC
GCGGGGAGGCCGAGGCTATTACTTCGTCACTCCCGCCCAGCCCCAGCCGCCGGTGGTCGCGGCGTTCGCCGCGTGGTTGC
AGGACATGGCCGCGACTTGA

Upstream 100 bases:

>100_bases
ACTCGGAATGCATGGATGCTATGCCTTGTGGATGGGCTGCGGCAAGCGATGAACTCGCATCCAGAAATGAGCTTTTGGAA
TACCTGTGTAGCATCCGGCC

Downstream 100 bases:

>100_bases
CCGCGACGGCGCCCTGTCCGGTTGCATCGCCCGCGACCGCGGGCTAGGCTGTGTCACACCTGCTGTAGAGCAGGCTTGCA
GGCTCTGTTTCCCTGCCTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRRTLPSTQALACFEAAARHESYTRAAQELFLTQSAVSRQIMALEEFLGVALFRRTRHGVALTPAGTHYAAQVGRWLEGL
ERDTLDVMAHQGQGGSIRLAAVPTFATRWLVPRLPRLAERHPDIVVHIETQVRPFLFADAGFDAALYAGTPEQVANWPGT
QALRLMQEDVAPVCSPALLPEGRPLPPEALAELPLLQQTTRPLAWRQWFESMGVHAPQHALDGPRYELFSMSAVAAAHGL
GVALVPLMLIEPERARGELVPACDRAPRGGRGYYFVTPAQPQPPVVAAFAAWLQDMAAT

Sequences:

>Translated_299_residues
MRRTLPSTQALACFEAAARHESYTRAAQELFLTQSAVSRQIMALEEFLGVALFRRTRHGVALTPAGTHYAAQVGRWLEGL
ERDTLDVMAHQGQGGSIRLAAVPTFATRWLVPRLPRLAERHPDIVVHIETQVRPFLFADAGFDAALYAGTPEQVANWPGT
QALRLMQEDVAPVCSPALLPEGRPLPPEALAELPLLQQTTRPLAWRQWFESMGVHAPQHALDGPRYELFSMSAVAAAHGL
GVALVPLMLIEPERARGELVPACDRAPRGGRGYYFVTPAQPQPPVVAAFAAWLQDMAAT
>Mature_299_residues
MRRTLPSTQALACFEAAARHESYTRAAQELFLTQSAVSRQIMALEEFLGVALFRRTRHGVALTPAGTHYAAQVGRWLEGL
ERDTLDVMAHQGQGGSIRLAAVPTFATRWLVPRLPRLAERHPDIVVHIETQVRPFLFADAGFDAALYAGTPEQVANWPGT
QALRLMQEDVAPVCSPALLPEGRPLPPEALAELPLLQQTTRPLAWRQWFESMGVHAPQHALDGPRYELFSMSAVAAAHGL
GVALVPLMLIEPERARGELVPACDRAPRGGRGYYFVTPAQPQPPVVAAFAAWLQDMAAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=36, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=31.6498316498317, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=31.8339100346021, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI145693193, Length=273, Percent_Identity=28.9377289377289, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=260, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=37.8151260504202, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32730; Mature: 32730

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTLPSTQALACFEAAARHESYTRAAQELFLTQSAVSRQIMALEEFLGVALFRRTRHGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
ALTPAGTHYAAQVGRWLEGLERDTLDVMAHQGQGGSIRLAAVPTFATRWLVPRLPRLAER
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHC
HPDIVVHIETQVRPFLFADAGFDAALYAGTPEQVANWPGTQALRLMQEDVAPVCSPALLP
CCCEEEEEECCCCEEEEECCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
EGRPLPPEALAELPLLQQTTRPLAWRQWFESMGVHAPQHALDGPRYELFSMSAVAAAHGL
CCCCCCHHHHHHCHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHCC
GVALVPLMLIEPERARGELVPACDRAPRGGRGYYFVTPAQPQPPVVAAFAAWLQDMAAT
HHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRTLPSTQALACFEAAARHESYTRAAQELFLTQSAVSRQIMALEEFLGVALFRRTRHGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
ALTPAGTHYAAQVGRWLEGLERDTLDVMAHQGQGGSIRLAAVPTFATRWLVPRLPRLAER
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHC
HPDIVVHIETQVRPFLFADAGFDAALYAGTPEQVANWPGTQALRLMQEDVAPVCSPALLP
CCCEEEEEECCCCEEEEECCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
EGRPLPPEALAELPLLQQTTRPLAWRQWFESMGVHAPQHALDGPRYELFSMSAVAAAHGL
CCCCCCHHHHHHCHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHCC
GVALVPLMLIEPERARGELVPACDRAPRGGRGYYFVTPAQPQPPVVAAFAAWLQDMAAT
HHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]