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The map label for this gene is ascD [H]

Identifier: 120610657

GI number: 120610657

Start: 2146151

End: 2147224

Strand: Reverse

Name: ascD [H]

Synonym: Aave_1977

Alternate gene names: 120610657

Gene position: 2147224-2146151 (Counterclockwise)

Preceding gene: 120610659

Following gene: 120610655

Centisome position: 40.11

GC content: 69.83

Gene sequence:

>1074_bases
ATGAACACCGCAGCGACGGCCGCAGCCTCCCACCAGATCACCGTGCAGCCCAGCGGCCGCGCCTTCACCGCCCAGGCCGG
CGAAACCCTGCTGGGCGCTGCCATCCGCAGCGGCGTGGGCCTGCCCTACGGCTGCAAGGACGGCGCCTGCGGTTCCTGCA
AATGCCGCAAGCTGAGCGGTTCGGTCGCGCACGGAGAGCACCAGTCCAAAGCCCTGACGCCCGAGGAGGCCGACGCGGGT
TTCGTGCTCACCTGCTGCGCGCAGCCGCTCACCGACGTGGTGCTGGAGTCGCGCCAGGTCACCGACGAAAGCGCCTACCC
CATCAAGAAGATGCCGGTGCGCGTCGCCGCCCTCGCGCGCGCCTCGCACGACGTGATGGTGGTTCGCCTGCAGCTGCCCG
CGGCCGACACGTTCCGCTACCACGCAGGCCAGTACGTGGAGTTCATCCTCAAGGACGGCGCACGCCGCGCTTATTCGATG
GCCAATGCGCCGCACACGCAGGAGGGCAGCCCGGGCATCGAGCTGCACATCCGCCACATGCCGGGCGGCCGGTTCACCGA
CCACGTGTTCGGCGCCATGAAGGAAAAGGAAATCCTGCGCGTGGAGGGCCCGTTCGGCAGCTTCTTCCTGCGCGAGGACA
GCCCCAAACCGATCATCCTGCTGGCCTCGGGCACGGGCTTCGCGCCGGTGAAGGCGCTCATCGAGCACCTGCGCTTCAAG
GGCATCGACCGCCCTGTCACGCTCTACTGGGGCGGCCGCCGGCCGGCGGACCTCTACATGGATGCCTGGGTCCGCGAGCG
CCTGGCGGACATGCCCGGGCTGCGCTACGTGCCGGTGGTGTCGCACGCCCTGCCGGAAGACGGCTGGACCGGCCGCACGG
GTTTCGTGCACCAGGCCGTGATGGACGATCACGCCGACCTGTCGGGACACCAGGTGTATGCCTGCGGGGCGCCGGTGGTG
GTGGAATCGGCGCGCAAGGCCTACAGTGCCGAGCGCGGCTTGCCCCCGGAAGAGTTCTACGCGGACGCCTTCACGTCCGA
GGCGGACAAGCACGGCGGCACCGTCGCCTCCTGA

Upstream 100 bases:

>100_bases
CGTTCACGGCGGAAACGCGCCGGCAGCGCGCCGAGGGGGTTCAGGTTTGAGGGCAAAATCCAGATCCGGCAACGAAGAGA
CACGCAAGGATAACGACCCC

Downstream 100 bases:

>100_bases
ACGGCGCGCGTCCCGGCAGGGCCCGGGCCGGCCGGACTGGAAGCCAGGTTCAGCCCATCGTGCGGCAGAGTTCCGCGCAG
CGCCGGCACATGGCGGCGCA

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNTAATAAASHQITVQPSGRAFTAQAGETLLGAAIRSGVGLPYGCKDGACGSCKCRKLSGSVAHGEHQSKALTPEEADAG
FVLTCCAQPLTDVVLESRQVTDESAYPIKKMPVRVAALARASHDVMVVRLQLPAADTFRYHAGQYVEFILKDGARRAYSM
ANAPHTQEGSPGIELHIRHMPGGRFTDHVFGAMKEKEILRVEGPFGSFFLREDSPKPIILLASGTGFAPVKALIEHLRFK
GIDRPVTLYWGGRRPADLYMDAWVRERLADMPGLRYVPVVSHALPEDGWTGRTGFVHQAVMDDHADLSGHQVYACGAPVV
VESARKAYSAERGLPPEEFYADAFTSEADKHGGTVAS

Sequences:

>Translated_357_residues
MNTAATAAASHQITVQPSGRAFTAQAGETLLGAAIRSGVGLPYGCKDGACGSCKCRKLSGSVAHGEHQSKALTPEEADAG
FVLTCCAQPLTDVVLESRQVTDESAYPIKKMPVRVAALARASHDVMVVRLQLPAADTFRYHAGQYVEFILKDGARRAYSM
ANAPHTQEGSPGIELHIRHMPGGRFTDHVFGAMKEKEILRVEGPFGSFFLREDSPKPIILLASGTGFAPVKALIEHLRFK
GIDRPVTLYWGGRRPADLYMDAWVRERLADMPGLRYVPVVSHALPEDGWTGRTGFVHQAVMDDHADLSGHQVYACGAPVV
VESARKAYSAERGLPPEEFYADAFTSEADKHGGTVAS
>Mature_357_residues
MNTAATAAASHQITVQPSGRAFTAQAGETLLGAAIRSGVGLPYGCKDGACGSCKCRKLSGSVAHGEHQSKALTPEEADAG
FVLTCCAQPLTDVVLESRQVTDESAYPIKKMPVRVAALARASHDVMVVRLQLPAADTFRYHAGQYVEFILKDGARRAYSM
ANAPHTQEGSPGIELHIRHMPGGRFTDHVFGAMKEKEILRVEGPFGSFFLREDSPKPIILLASGTGFAPVKALIEHLRFK
GIDRPVTLYWGGRRPADLYMDAWVRERLADMPGLRYVPVVSHALPEDGWTGRTGFVHQAVMDDHADLSGHQVYACGAPVV
VESARKAYSAERGLPPEEFYADAFTSEADKHGGTVAS

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=237, Percent_Identity=32.4894514767932, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787658, Length=90, Percent_Identity=44.4444444444444, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322699, Length=243, Percent_Identity=24.2798353909465, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 38499; Mature: 38499

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAATAAASHQITVQPSGRAFTAQAGETLLGAAIRSGVGLPYGCKDGACGSCKCRKLSG
CCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
SVAHGEHQSKALTPEEADAGFVLTCCAQPLTDVVLESRQVTDESAYPIKKMPVRVAALAR
CCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
ASHDVMVVRLQLPAADTFRYHAGQYVEFILKDGARRAYSMANAPHTQEGSPGIELHIRHM
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEEEEEEC
PGGRFTDHVFGAMKEKEILRVEGPFGSFFLREDSPKPIILLASGTGFAPVKALIEHLRFK
CCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHC
GIDRPVTLYWGGRRPADLYMDAWVRERLADMPGLRYVPVVSHALPEDGWTGRTGFVHQAV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCCCCCCHHHHHH
MDDHADLSGHQVYACGAPVVVESARKAYSAERGLPPEEFYADAFTSEADKHGGTVAS
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTAATAAASHQITVQPSGRAFTAQAGETLLGAAIRSGVGLPYGCKDGACGSCKCRKLSG
CCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
SVAHGEHQSKALTPEEADAGFVLTCCAQPLTDVVLESRQVTDESAYPIKKMPVRVAALAR
CCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
ASHDVMVVRLQLPAADTFRYHAGQYVEFILKDGARRAYSMANAPHTQEGSPGIELHIRHM
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEEEEEEC
PGGRFTDHVFGAMKEKEILRVEGPFGSFFLREDSPKPIILLASGTGFAPVKALIEHLRFK
CCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHC
GIDRPVTLYWGGRRPADLYMDAWVRERLADMPGLRYVPVVSHALPEDGWTGRTGFVHQAV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCCCCCCCHHHHHH
MDDHADLSGHQVYACGAPVVVESARKAYSAERGLPPEEFYADAFTSEADKHGGTVAS
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]