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The map label for this gene is rpoN [H]

Identifier: 120610579

GI number: 120610579

Start: 2054974

End: 2056380

Strand: Reverse

Name: rpoN [H]

Synonym: Aave_1899

Alternate gene names: 120610579

Gene position: 2056380-2054974 (Counterclockwise)

Preceding gene: 120610589

Following gene: 120610578

Centisome position: 38.42

GC content: 70.65

Gene sequence:

>1407_bases
ATGCCGACACTCGCTCTTGGAGCCCGACAGACCCAGACCCCGCACCTTTCGCCCCGCCTCCAGAAGGCTGTGCAATTGCT
GCAGATGCCGACGGCGGAGTTCATCCAGAGCGTGATGGCTGCCGTGGACGAGAACCCGTTCCTCGAGGAAGACGAGGCGG
CGGCACCTGCTGCCCCCGGCCCGGAAGAGGGGCCGGCCTGGACCGCGGCACCGGGCTCCCGGTCGCCCCAGGCCGGCAGC
GGCGACGGCCTGTCCATGCTGGACCGCGAACCGGCGACGGCCACGCTGCAGGAACACCTGCACTCGCAGTTGCGCCTGCT
GCGCCTGCCCGACCGCGCCTTTCTCCTGGCGAGCCTGGTGGTCGAAGAGTTGTCCGATGACGGCTACCTGCTGGCCTCCC
TGGACTCCCTCTGCAGGGACTACGGCGTGGAGCCGGAGGCCACGGCCGAGGAGCTGGACGCCGCGCTGCGCACCGTGCAG
TCCCTGGAGCCCGCGGGCGTGGGTGCGCGCGACCTGCAGGAATGCCTGCGCCTGCAGCTGCCCGCAGTCGCCTGCCCGCT
GCAGCGTGCGCTGTGCGATGCGGTACTGGCCAGGGCGGCGGAAGGGTCCGGGCCGCAGGCCATGCGGCAGCTGGCGGCAC
GCCTGGATGTACCCGCGCATGCCGTGCGCGAGGCAATGGAGCGGCTGCGGCACCTCGACCCCCACCCGGGATGGCGGCAC
GGGTCCCAGCCGGTGCAGTACATCATGCCCGATGTCATCGCGCACAAGGCCCGCGGGCAGTGGACCGTCTCGCTGAACGA
GGCCGTCATGCCGCGCGTGCGCGTCCATCGCCAGTACCAGCAGTTGCTTCAGTCGCCGGCCGATGCGCCGGACGGGCCGG
CGCCCGCTCCGGAAGACACCGGAGCGCGCAACGCGCACCGCGATCTGGCGGCGCAACTGGCCGAGGCGCGCTGGACCGTC
CGCCATGTGCAGCAGCGCTTTTCCACCGTGCTGGATGTCGCCAGGGCGATCCTGCGCCGGCAGCCCCACTTCCTGGAATA
CGGCCCCATCGGCATGCGACCGCTGGCGCTCAAGGACATCGCGGAAGAATTGGGGGTGCATGTCTCCACCGTATCGCGGG
TGACGAACAACAAGTACCTGCAGACGCCGTTCGGCTTGTTCGAACTGAAGTACTTCTTCTCCCGCGCCATGACCACGCCG
GCAGGGCGGGACAGTTCGCCCACCGCCATCCGCGGGCTCGTGCAGGAAATCCTGGCGGCGGACGACGGCCAGCGGCCGCT
CTCCGACGTGGACATCGCACGGCAGCTCGAGCGCCAGGGTATCCGCATCGCGCGCCGCACGGTGACCAAGTACCGGCAGC
AACTGCGCATTCCGCCCGCAGAGCAGCGTGCCGCGGCGCGTCCCTGA

Upstream 100 bases:

>100_bases
AGGTATCCATACGTACACTGTCGGACAAAGGCGGCATGGTGTTTGCACGAAGCATTGGAAGGCCACACATGGCTGGCCTG
CCCGAGGAACCTTTCGTCCG

Downstream 100 bases:

>100_bases
AGCTGCAGGCGCCTCCGCGGCCGCAAGGGGCCCCCGGCAGCGGGGACGGCCCATAATCACCGGCGCACCATGCCGTTCCT
CCGCAGCGCCCTCGCCGTCC

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPTLALGARQTQTPHLSPRLQKAVQLLQMPTAEFIQSVMAAVDENPFLEEDEAAAPAAPGPEEGPAWTAAPGSRSPQAGS
GDGLSMLDREPATATLQEHLHSQLRLLRLPDRAFLLASLVVEELSDDGYLLASLDSLCRDYGVEPEATAEELDAALRTVQ
SLEPAGVGARDLQECLRLQLPAVACPLQRALCDAVLARAAEGSGPQAMRQLAARLDVPAHAVREAMERLRHLDPHPGWRH
GSQPVQYIMPDVIAHKARGQWTVSLNEAVMPRVRVHRQYQQLLQSPADAPDGPAPAPEDTGARNAHRDLAAQLAEARWTV
RHVQQRFSTVLDVARAILRRQPHFLEYGPIGMRPLALKDIAEELGVHVSTVSRVTNNKYLQTPFGLFELKYFFSRAMTTP
AGRDSSPTAIRGLVQEILAADDGQRPLSDVDIARQLERQGIRIARRTVTKYRQQLRIPPAEQRAAARP

Sequences:

>Translated_468_residues
MPTLALGARQTQTPHLSPRLQKAVQLLQMPTAEFIQSVMAAVDENPFLEEDEAAAPAAPGPEEGPAWTAAPGSRSPQAGS
GDGLSMLDREPATATLQEHLHSQLRLLRLPDRAFLLASLVVEELSDDGYLLASLDSLCRDYGVEPEATAEELDAALRTVQ
SLEPAGVGARDLQECLRLQLPAVACPLQRALCDAVLARAAEGSGPQAMRQLAARLDVPAHAVREAMERLRHLDPHPGWRH
GSQPVQYIMPDVIAHKARGQWTVSLNEAVMPRVRVHRQYQQLLQSPADAPDGPAPAPEDTGARNAHRDLAAQLAEARWTV
RHVQQRFSTVLDVARAILRRQPHFLEYGPIGMRPLALKDIAEELGVHVSTVSRVTNNKYLQTPFGLFELKYFFSRAMTTP
AGRDSSPTAIRGLVQEILAADDGQRPLSDVDIARQLERQGIRIARRTVTKYRQQLRIPPAEQRAAARP
>Mature_467_residues
PTLALGARQTQTPHLSPRLQKAVQLLQMPTAEFIQSVMAAVDENPFLEEDEAAAPAAPGPEEGPAWTAAPGSRSPQAGSG
DGLSMLDREPATATLQEHLHSQLRLLRLPDRAFLLASLVVEELSDDGYLLASLDSLCRDYGVEPEATAEELDAALRTVQS
LEPAGVGARDLQECLRLQLPAVACPLQRALCDAVLARAAEGSGPQAMRQLAARLDVPAHAVREAMERLRHLDPHPGWRHG
SQPVQYIMPDVIAHKARGQWTVSLNEAVMPRVRVHRQYQQLLQSPADAPDGPAPAPEDTGARNAHRDLAAQLAEARWTVR
HVQQRFSTVLDVARAILRRQPHFLEYGPIGMRPLALKDIAEELGVHVSTVSRVTNNKYLQTPFGLFELKYFFSRAMTTPA
GRDSSPTAIRGLVQEILAADDGQRPLSDVDIARQLERQGIRIARRTVTKYRQQLRIPPAEQRAAARP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=491, Percent_Identity=33.1975560081466, Blast_Score=260, Evalue=2e-70,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51376; Mature: 51245

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLALGARQTQTPHLSPRLQKAVQLLQMPTAEFIQSVMAAVDENPFLEEDEAAAPAAPG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PEEGPAWTAAPGSRSPQAGSGDGLSMLDREPATATLQEHLHSQLRLLRLPDRAFLLASLV
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VEELSDDGYLLASLDSLCRDYGVEPEATAEELDAALRTVQSLEPAGVGARDLQECLRLQL
HHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PAVACPLQRALCDAVLARAAEGSGPQAMRQLAARLDVPAHAVREAMERLRHLDPHPGWRH
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
GSQPVQYIMPDVIAHKARGQWTVSLNEAVMPRVRVHRQYQQLLQSPADAPDGPAPAPEDT
CCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GARNAHRDLAAQLAEARWTVRHVQQRFSTVLDVARAILRRQPHFLEYGPIGMRPLALKDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEELGVHVSTVSRVTNNKYLQTPFGLFELKYFFSRAMTTPAGRDSSPTAIRGLVQEILAA
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
DDGQRPLSDVDIARQLERQGIRIARRTVTKYRQQLRIPPAEQRAAARP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
>Mature Secondary Structure 
PTLALGARQTQTPHLSPRLQKAVQLLQMPTAEFIQSVMAAVDENPFLEEDEAAAPAAPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PEEGPAWTAAPGSRSPQAGSGDGLSMLDREPATATLQEHLHSQLRLLRLPDRAFLLASLV
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VEELSDDGYLLASLDSLCRDYGVEPEATAEELDAALRTVQSLEPAGVGARDLQECLRLQL
HHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PAVACPLQRALCDAVLARAAEGSGPQAMRQLAARLDVPAHAVREAMERLRHLDPHPGWRH
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
GSQPVQYIMPDVIAHKARGQWTVSLNEAVMPRVRVHRQYQQLLQSPADAPDGPAPAPEDT
CCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GARNAHRDLAAQLAEARWTVRHVQQRFSTVLDVARAILRRQPHFLEYGPIGMRPLALKDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEELGVHVSTVSRVTNNKYLQTPFGLFELKYFFSRAMTTPAGRDSSPTAIRGLVQEILAA
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
DDGQRPLSDVDIARQLERQGIRIARRTVTKYRQQLRIPPAEQRAAARP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]