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The map label for this gene is bcr [H]

Identifier: 120610576

GI number: 120610576

Start: 2052581

End: 2053813

Strand: Reverse

Name: bcr [H]

Synonym: Aave_1896

Alternate gene names: 120610576

Gene position: 2053813-2052581 (Counterclockwise)

Preceding gene: 120610577

Following gene: 120610572

Centisome position: 38.37

GC content: 69.18

Gene sequence:

>1233_bases
ATGAATCCAGACGCCCACCATCTCTGGCGCGCCCCGCGCTGGACCCTCGCCGTCCTGCTGGCCGTGCTGGGCATGCTGGG
GCCGTTCTCGATCGATACCTACATACCGGCGTTCTCGGGCATCGCGGCGGCGCTGGGCGCCACGCCGGTCGAGATGCAGC
AGACGCTGTCGGCCTACCTGTTCGGCTTCGCGTTCATGACGCTGTTCCACGGCGCCCTGTCCGACAGCTTCGGGCGCAGG
CCGGTGGTGCTCTGGGGCATCGCCGTGTTCACGCTGGCTTCCGCGGGCTGCGCACTGTCGCAAAGCATCGGCCAGCTGGT
GCTGTTCCGCGCGCTGCAGGGCCTGTCGGCCGGCGCGGGCATCGTGGTTTCGCGGGCGGTGATCCGCGACATGTTCCCGC
CCGCCCAGGCCCAGCAGGTGATGAGCCAGGTCACCATCTATTTCGGCGTGGCGCCGGCCATCGCCCCCATCGTGGGCGGC
TGGCTGTTCGTCCATGCGGGCTGGCACAGCGTGTTCTGGTTCCTCACCGGCGTGGGGCTGCTGCTCTGGATCGCCAACTA
CCGCCTGCTGCCCGAGACGCTGCATGCGGACCACCGCCAGCCCTTCAACGCCCGCCACCTGCTGCAGGGCTACTGGCAGC
TGGGCTCCAGCCCGCGCTTCCTGCTGCTGGCGCTCGCCAGCGGCGTGCCGTTCAACGGCATGTTCCTCTACGTGCTCGCG
GCCCCCGCCTTCCTCGGCGAGCACCTTCACCTTTCGCCCACGCATTTCTTCTGGTTCTTCATACTGACCATCTCGGGCAT
CATGGGCGGCGCCTGGCTCAGCGGCCGCCTGGCCGGCCGCATCGCGCCCAAGCGCCAGATACGGCACGGCTTCCTCATCA
TGTTCATCATGTCCATGCTGAACCTGGCGGCCAATGCACTGTTCACGGCCCACGTCTCGTGGGCGCTGCTGCCCATCGCG
GTCTTCGCCTTCGGATGGGCGCTGATGGTGCCGGTCGTCACCCTGCTCGTGCTGGACCTGTACCCCGAGCGCCGCGGCAT
GGCCTCGTCGCTGCAGGCCTTCGTCGGCTCCACCGCCAACGGCCTGGTGGCGGGCGTGGTGGCCCCGCTGGTGATGCACT
CCACCCTGCTGCTGGCCTGCGCGTCGCTGGCGATGATGTGCGTGGGCCTGGTGGCCTGGGTCTATCTGCACCACCGCTGG
CCGGACATCGGCCGCGTGGCCACCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGCCTGCGGCGGCGCCCCGATAATGGAAATCGCCCGCCTGCCGGTGCTCCGCACCGTGCGCGGGCCGGCCCGACAGCCC
CCATCCCCCCTGCTTCCGCC

Downstream 100 bases:

>100_bases
AGCAGGCGCGGCCTGCCGCACCGTGGCAGCTTCGTGCGCCCGTGCTCGCGGCCGGAGGTGCCCGCGTCAGCTGCGGCGGC
GCTCCAGCCCGGCCTGCTGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNPDAHHLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIAAALGATPVEMQQTLSAYLFGFAFMTLFHGALSDSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVLFRALQGLSAGAGIVVSRAVIRDMFPPAQAQQVMSQVTIYFGVAPAIAPIVGG
WLFVHAGWHSVFWFLTGVGLLLWIANYRLLPETLHADHRQPFNARHLLQGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGEHLHLSPTHFFWFFILTISGIMGGAWLSGRLAGRIAPKRQIRHGFLIMFIMSMLNLAANALFTAHVSWALLPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFVGSTANGLVAGVVAPLVMHSTLLLACASLAMMCVGLVAWVYLHHRW
PDIGRVATAA

Sequences:

>Translated_410_residues
MNPDAHHLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIAAALGATPVEMQQTLSAYLFGFAFMTLFHGALSDSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVLFRALQGLSAGAGIVVSRAVIRDMFPPAQAQQVMSQVTIYFGVAPAIAPIVGG
WLFVHAGWHSVFWFLTGVGLLLWIANYRLLPETLHADHRQPFNARHLLQGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGEHLHLSPTHFFWFFILTISGIMGGAWLSGRLAGRIAPKRQIRHGFLIMFIMSMLNLAANALFTAHVSWALLPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFVGSTANGLVAGVVAPLVMHSTLLLACASLAMMCVGLVAWVYLHHRW
PDIGRVATAA
>Mature_410_residues
MNPDAHHLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIAAALGATPVEMQQTLSAYLFGFAFMTLFHGALSDSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVLFRALQGLSAGAGIVVSRAVIRDMFPPAQAQQVMSQVTIYFGVAPAIAPIVGG
WLFVHAGWHSVFWFLTGVGLLLWIANYRLLPETLHADHRQPFNARHLLQGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGEHLHLSPTHFFWFFILTISGIMGGAWLSGRLAGRIAPKRQIRHGFLIMFIMSMLNLAANALFTAHVSWALLPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFVGSTANGLVAGVVAPLVMHSTLLLACASLAMMCVGLVAWVYLHHRW
PDIGRVATAA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=340, Percent_Identity=37.6470588235294, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI48994889, Length=390, Percent_Identity=29.4871794871795, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87082312, Length=347, Percent_Identity=28.5302593659942, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790146, Length=194, Percent_Identity=31.4432989690722, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790794, Length=324, Percent_Identity=25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790195, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788392, Length=172, Percent_Identity=33.7209302325581, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787065, Length=280, Percent_Identity=26.4285714285714, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081738, Length=181, Percent_Identity=32.0441988950276, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789042, Length=144, Percent_Identity=27.0833333333333, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=182, Percent_Identity=25.8241758241758, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322070, Length=125, Percent_Identity=30.4, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=179, Percent_Identity=27.3743016759777, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44442; Mature: 44442

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]