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The map label for this gene is ynfL [H]

Identifier: 120610522

GI number: 120610522

Start: 1996826

End: 1997749

Strand: Direct

Name: ynfL [H]

Synonym: Aave_1842

Alternate gene names: 120610522

Gene position: 1996826-1997749 (Clockwise)

Preceding gene: 120610518

Following gene: 120610529

Centisome position: 37.3

GC content: 70.02

Gene sequence:

>924_bases
ATGGAATTGAGGCACCTGCGCTACTTCGCGGTACTCGCGGACGAACTGCACTTCGGGCGCGCCGCGGCCCGGTTGAACAT
GTCGCAGCCACCGCTCTCGGTGGCCATCCGGCAACTGGAGGAGCGCGTCGGAGCGCAACTCTTCGAACGGACCCCCAAGG
AGGTGCGCCTCACCCCTGCCGGCCGCGCCCTCAAGGCGTCCGCGCGGCGGGTGCTGGCGCAAGCGGAGGAGGCGTTATCC
GAAGCGCGCGGCGTGGCTGCAGGGCTCGCAGGCCGGCTGCGCATCGGCTTCGTGGGCGCCATGCTGTACCGCGGACTGCC
GCAGGCGCTGGGTGTTTTCCAGCAGCGCCATCCGGGGGTGCGTGTTTCGCTGCAGGAATTGAACTCCGGTGAGCAGATCG
GCGAACTGCTGCACGACCGGCTGGACCTGGGCTTCGTCCATACGAGCCGCATGCCGGCGACGCTGCAGCAGCGCCTGCTG
GTGACCGAGCCCTTCCTGTGCTGCCTGCCTGCGGGGCATCGACTGGCGCGCAGGAAGGTCATCGATCTGCGCGATCTCGC
GGGCGAGCCCTTCGTCCTGTTCGCGCGCGCAGTCTCGCCCGACTACCATGCGCGCATCCTGTCGATCTGCGCGAGCGCGG
GCTTCCAGCCGGAAATAGGGCACGAGGTCCGGCACTGGCTCGCGGTGGTCTCCCTGGTCTCGCAAGGCCTCGGCGTCGCC
CTGGTGCCGCAGGCCATGGAGCATTCACGCCTGCCAGGAGCGGTGTTCCGGCCGGTGGGCCCTGCCGTGGCCCAGTCCGA
GGCCTACGCCGTATGGCCCGCGGCACCGGAGAATCCGTTGGTCGGCGATCTGATGGCGATCCTCCTGGCCGGTGGGCCGG
CAGGCTCCAGCGGGCGCCGGGAGCACGGTGGCAGTGCTCCATGA

Upstream 100 bases:

>100_bases
TGGTGTCTCCTTGTCGATTTCCCATTCTTCGCCGCGTGGCCATTCGTGTCTAATATCGATTAGGTCTTGCGCCATTCGAA
GCTCCTATCGCCATACCGTC

Downstream 100 bases:

>100_bases
AAAAACCCCTGACTCTTGCGAATCAGGGGTTTTTTGTTTGGTGCCGGAGAGATGAATCGAACACCCGACCTTCTCATTAC
GAATGAGCTGCTCTACCGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MELRHLRYFAVLADELHFGRAAARLNMSQPPLSVAIRQLEERVGAQLFERTPKEVRLTPAGRALKASARRVLAQAEEALS
EARGVAAGLAGRLRIGFVGAMLYRGLPQALGVFQQRHPGVRVSLQELNSGEQIGELLHDRLDLGFVHTSRMPATLQQRLL
VTEPFLCCLPAGHRLARRKVIDLRDLAGEPFVLFARAVSPDYHARILSICASAGFQPEIGHEVRHWLAVVSLVSQGLGVA
LVPQAMEHSRLPGAVFRPVGPAVAQSEAYAVWPAAPENPLVGDLMAILLAGGPAGSSGRREHGGSAP

Sequences:

>Translated_307_residues
MELRHLRYFAVLADELHFGRAAARLNMSQPPLSVAIRQLEERVGAQLFERTPKEVRLTPAGRALKASARRVLAQAEEALS
EARGVAAGLAGRLRIGFVGAMLYRGLPQALGVFQQRHPGVRVSLQELNSGEQIGELLHDRLDLGFVHTSRMPATLQQRLL
VTEPFLCCLPAGHRLARRKVIDLRDLAGEPFVLFARAVSPDYHARILSICASAGFQPEIGHEVRHWLAVVSLVSQGLGVA
LVPQAMEHSRLPGAVFRPVGPAVAQSEAYAVWPAAPENPLVGDLMAILLAGGPAGSSGRREHGGSAP
>Mature_307_residues
MELRHLRYFAVLADELHFGRAAARLNMSQPPLSVAIRQLEERVGAQLFERTPKEVRLTPAGRALKASARRVLAQAEEALS
EARGVAAGLAGRLRIGFVGAMLYRGLPQALGVFQQRHPGVRVSLQELNSGEQIGELLHDRLDLGFVHTSRMPATLQQRLL
VTEPFLCCLPAGHRLARRKVIDLRDLAGEPFVLFARAVSPDYHARILSICASAGFQPEIGHEVRHWLAVVSLVSQGLGVA
LVPQAMEHSRLPGAVFRPVGPAVAQSEAYAVWPAAPENPLVGDLMAILLAGGPAGSSGRREHGGSAP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=278, Percent_Identity=39.2086330935252, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI2367136, Length=289, Percent_Identity=34.6020761245675, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=34.2465753424658, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=28.9156626506024, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=32.1138211382114, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=28.4552845528455, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=25.9259259259259, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787601, Length=295, Percent_Identity=27.1186440677966, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787806, Length=274, Percent_Identity=25.5474452554745, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=263, Percent_Identity=29.277566539924, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=26.8595041322314, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788297, Length=261, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=32.6530612244898, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=258, Percent_Identity=27.1317829457364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=212, Percent_Identity=28.3018867924528, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787589, Length=143, Percent_Identity=32.1678321678322, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33118; Mature: 33118

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFAVLADELHFGRAAARLNMSQPPLSVAIRQLEERVGAQLFERTPKEVRLTPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCC
GRALKASARRVLAQAEEALSEARGVAAGLAGRLRIGFVGAMLYRGLPQALGVFQQRHPGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
RVSLQELNSGEQIGELLHDRLDLGFVHTSRMPATLQQRLLVTEPFLCCLPAGHRLARRKV
EEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHH
IDLRDLAGEPFVLFARAVSPDYHARILSICASAGFQPEIGHEVRHWLAVVSLVSQGLGVA
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LVPQAMEHSRLPGAVFRPVGPAVAQSEAYAVWPAAPENPLVGDLMAILLAGGPAGSSGRR
ECHHHHHHCCCCCHHHCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCC
EHGGSAP
CCCCCCC
>Mature Secondary Structure
MELRHLRYFAVLADELHFGRAAARLNMSQPPLSVAIRQLEERVGAQLFERTPKEVRLTPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCC
GRALKASARRVLAQAEEALSEARGVAAGLAGRLRIGFVGAMLYRGLPQALGVFQQRHPGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
RVSLQELNSGEQIGELLHDRLDLGFVHTSRMPATLQQRLLVTEPFLCCLPAGHRLARRKV
EEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHH
IDLRDLAGEPFVLFARAVSPDYHARILSICASAGFQPEIGHEVRHWLAVVSLVSQGLGVA
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LVPQAMEHSRLPGAVFRPVGPAVAQSEAYAVWPAAPENPLVGDLMAILLAGGPAGSSGRR
ECHHHHHHCCCCCHHHCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCC
EHGGSAP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]