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The map label for this gene is iaaA [H]

Identifier: 120610339

GI number: 120610339

Start: 1815687

End: 1816679

Strand: Direct

Name: iaaA [H]

Synonym: Aave_1656

Alternate gene names: 120610339

Gene position: 1815687-1816679 (Clockwise)

Preceding gene: 120610338

Following gene: 120610340

Centisome position: 33.92

GC content: 75.63

Gene sequence:

>993_bases
ATGACCGCCTTCGCCGCATCCCCGTCCGCCGTCCGCCCCGTGCCCGTAATCGCCATCCATGGCGGCGCGGGCACGCTGAA
CCGCGCGCACATCAGCCCCGCCCAGGAGCAGGCCTACCATGCCGCGCTGCAGGACGTGCTGCGCGCGGGCCAGGCCGTGC
TGGCGCGGGGCGGCAGCGCGGTGGATGCCGTCTGCGAAGCCGTGCGCCTGCTGGAGGAGTGCCCGCTGTTCAACGCCGGC
CATGGCGCGGTGTTCACGGCCGACGCCACGCACGAACTGGACGCGGCGGTGATGGACGGCGCGAACCTGGCGGCCGGCGC
GGTGGCGGGCGTGGCCCACGTGCGCAACCCGGTGCTGGCGGCCCGCGCGGTGATGCGACACGGCCAGCACGTGCTCATGG
CCGGCGAGGGCGCAGAGCGCATCGCACGCGACGCGGGCCTGGAGATGGTGGAGCCGTCGCATTTCTCGACCGATGCGCGC
CGCGCGCAGCTGGAGGCCGCGCGCGCCAGCCAGCGCGGCGCCGTGCTGGACCACGACGGCGCCGCGGCGCTGGCGGGCCG
CGCGCTCGACGAAGACCGCAAGATGGGCACGGTGGGTGCCGTGGCGCTGGACGCGCACGGGCACCTCGCCGCCGCCACCT
CCACGGGCGGCATGACCAACAAGCGCCCCGGCCGCGTGGGCGACAGCCCGCTGATCGGCGCGGGCACCTATGCCGACGAC
CGCACGGCGGCCGTCTCCTGCACCGGGCACGGCGAGAGCTTCATCCGCGTGGCGGCCGCGCACGACGTCTGCGCGCGCAT
GGCATACGGCGGCCAGGACCTCGCGCAGGCCGCCGATGCCGTGGTGCACGGCGCCCTGGCCGCCATCGGCGGCACCGGCG
GCCTGATCGCCGTGGACCGCCTGGGCAACGTGCGCCTGCCGTTCAATACCGAGGGCATGTACCGCGGATCCGCCCGCGTG
GGCGAAGCGCCCGAGACCTCCATCTACCGCTGA

Upstream 100 bases:

>100_bases
CCAACGCACCGCCTCCAGCCGCTTCGCCCGTACCGTCCGCTTCGTTCCGTTCCGTTCCCGTCCGCACGCTTTCCTTTCGC
CGCTTCCGCCGACCGCCCGC

Downstream 100 bases:

>100_bases
GGGCGCAGCGCCCCGTTCCTCCGCCCCACGACCGACCTTTCTTTCCGCCCGCCATGAAACCCAACGCCTCGACCGCCTCC
ACGACCCTCGCCCTGCCCGA

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTAFAASPSAVRPVPVIAIHGGAGTLNRAHISPAQEQAYHAALQDVLRAGQAVLARGGSAVDAVCEAVRLLEECPLFNAG
HGAVFTADATHELDAAVMDGANLAAGAVAGVAHVRNPVLAARAVMRHGQHVLMAGEGAERIARDAGLEMVEPSHFSTDAR
RAQLEAARASQRGAVLDHDGAAALAGRALDEDRKMGTVGAVALDAHGHLAAATSTGGMTNKRPGRVGDSPLIGAGTYADD
RTAAVSCTGHGESFIRVAAAHDVCARMAYGGQDLAQAADAVVHGALAAIGGTGGLIAVDRLGNVRLPFNTEGMYRGSARV
GEAPETSIYR

Sequences:

>Translated_330_residues
MTAFAASPSAVRPVPVIAIHGGAGTLNRAHISPAQEQAYHAALQDVLRAGQAVLARGGSAVDAVCEAVRLLEECPLFNAG
HGAVFTADATHELDAAVMDGANLAAGAVAGVAHVRNPVLAARAVMRHGQHVLMAGEGAERIARDAGLEMVEPSHFSTDAR
RAQLEAARASQRGAVLDHDGAAALAGRALDEDRKMGTVGAVALDAHGHLAAATSTGGMTNKRPGRVGDSPLIGAGTYADD
RTAAVSCTGHGESFIRVAAAHDVCARMAYGGQDLAQAADAVVHGALAAIGGTGGLIAVDRLGNVRLPFNTEGMYRGSARV
GEAPETSIYR
>Mature_329_residues
TAFAASPSAVRPVPVIAIHGGAGTLNRAHISPAQEQAYHAALQDVLRAGQAVLARGGSAVDAVCEAVRLLEECPLFNAGH
GAVFTADATHELDAAVMDGANLAAGAVAGVAHVRNPVLAARAVMRHGQHVLMAGEGAERIARDAGLEMVEPSHFSTDARR
AQLEAARASQRGAVLDHDGAAALAGRALDEDRKMGTVGAVALDAHGHLAAATSTGGMTNKRPGRVGDSPLIGAGTYADDR
TAAVSCTGHGESFIRVAAAHDVCARMAYGGQDLAQAADAVVHGALAAIGGTGGLIAVDRLGNVRLPFNTEGMYRGSARVG
EAPETSIYR

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=308, Percent_Identity=37.012987012987, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI145275200, Length=308, Percent_Identity=37.012987012987, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI98991769, Length=272, Percent_Identity=31.6176470588235, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI285002251, Length=262, Percent_Identity=32.0610687022901, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787050, Length=315, Percent_Identity=62.8571428571429, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32564649, Length=262, Percent_Identity=26.3358778625954, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=34.5794392523364, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=31.2267657992565, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI221330102, Length=234, Percent_Identity=34.6153846153846, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24652135, Length=234, Percent_Identity=34.6153846153846, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33486; Mature: 33355

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFAASPSAVRPVPVIAIHGGAGTLNRAHISPAQEQAYHAALQDVLRAGQAVLARGGSA
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCH
VDAVCEAVRLLEECPLFNAGHGAVFTADATHELDAAVMDGANLAAGAVAGVAHVRNPVLA
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHH
ARAVMRHGQHVLMAGEGAERIARDAGLEMVEPSHFSTDARRAQLEAARASQRGAVLDHDG
HHHHHHCCCEEEEECCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCEEECCC
AAALAGRALDEDRKMGTVGAVALDAHGHLAAATSTGGMTNKRPGRVGDSPLIGAGTYADD
HHHHHCCHHCHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCC
RTAAVSCTGHGESFIRVAAAHDVCARMAYGGQDLAQAADAVVHGALAAIGGTGGLIAVDR
CCEEEEECCCCCHHEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
LGNVRLPFNTEGMYRGSARVGEAPETSIYR
CCCEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAFAASPSAVRPVPVIAIHGGAGTLNRAHISPAQEQAYHAALQDVLRAGQAVLARGGSA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCH
VDAVCEAVRLLEECPLFNAGHGAVFTADATHELDAAVMDGANLAAGAVAGVAHVRNPVLA
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHH
ARAVMRHGQHVLMAGEGAERIARDAGLEMVEPSHFSTDARRAQLEAARASQRGAVLDHDG
HHHHHHCCCEEEEECCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCEEECCC
AAALAGRALDEDRKMGTVGAVALDAHGHLAAATSTGGMTNKRPGRVGDSPLIGAGTYADD
HHHHHCCHHCHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCC
RTAAVSCTGHGESFIRVAAAHDVCARMAYGGQDLAQAADAVVHGALAAIGGTGGLIAVDR
CCEEEEECCCCCHHEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
LGNVRLPFNTEGMYRGSARVGEAPETSIYR
CCCEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]