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The map label for this gene is nfdA [H]

Identifier: 120609454

GI number: 120609454

Start: 814599

End: 816491

Strand: Direct

Name: nfdA [H]

Synonym: Aave_0760

Alternate gene names: 120609454

Gene position: 814599-816491 (Clockwise)

Preceding gene: 120609453

Following gene: 120609455

Centisome position: 15.22

GC content: 70.21

Gene sequence:

>1893_bases
ATGACCACACCCGCCACCACTGCCGACCTGATCCTGCGCAACGGCCGCTTCACCACGCTGGACCGCGCCAATCCCACGGC
GAGCGCCGTCGCCATCGCGCAGGGGCGCTTCACCCGGGTGGGTGGCGACCGCGAAGTCATGGCGCTGGCCGGGCCGCAGA
CGCGGGTGATCGACCTGGGCGGGCGCAGCGTGCTGCCGGGGCTGATCGACAACCACCTGCACATCATCCGCGGCGGGCTG
AACTTCAACATGGAGCTGCGCTGGGACGGGGTGAAGAGCCTGGCCGATGCGATGGCGATGCTCAAGCGCCAGGTGGACGT
GACGCCCGCGCCGCAGTGGGTGCGGGTGGTGGGCGGCTTCACCGAGCACCAGTTCGTGGAAAAGCGCCTGCCCACGCTGG
CGGAGCTGAACGCCGTGGCGCCGGACACGCCGGTGTTCATCCTGCACCTGTACGACCGCGCGCTGCTCAACGGCGCCGCG
CTGCGGGCCGTGGGCTACGGCCGCGACACGCCCGCGCCGCCCGGCGGCGAGATCGTGCGCGACAGCGCGGGCAACCCCAC
GGGCCTGCTGCTGGCCAAGCCCAATGCGGCCATCCTGTACGCCACGCTGGCCAAGGGCCCGAAGCTGCCGCACGAATACC
AGGTGAACTCCACGCGCCACTTCATGCGCGAGCTCAACCGCCTGGGCGTGACCGGCGCGATCGACGCGGGCGGCGGCATG
CAGAACTACCCCGACGACTACCAGGTGATCCAGGAACTGGCCGACGCCGGCCAGCTCACGATCCGCCTGGCCTACAACCT
GTTCACGCAAAAGCCCAAGGAAGAGAAGGCCGACTTCCTGCACTGGACGGCCACCTCGCAGTACAAGCAGGGCACGGATT
ACTTCCGCCACAACGGCGCGGGCGAGATGCTGGTGTTCTCGGCCGCGGACTTCGAGGATTTCCGGCAGCCGCGCCCCGAG
CTGGCGCCGGAGATGGAAGGCGAGCTGGAGGAGGTGGTGCGCATCCTGGCGCAGAACCGCTGGCCCTGGCGCATGCACGC
CACCTACGACGAGACCATCGATCGCGCGCTCGGCGTGTTCGAGAAGGTGAACCGGGACACGCCGCTGGCCGGCCTGAACT
GGTTCTTCGACCACGCCGAGACCATCTCGGAAAAATCCATCGACCGCATCGCGGCCCTGGGCGGCGGCGTGGCCGTGCAG
CACCGCATGGCCTACCAGGGCGAGTATTTCATGGAGCGCTACGGCGCCGCCGCGGCAGAGGCCACGCCGCCCGTCAAGCG
CATGCTGGAGAAGGGCGTTCAAGTCTCCGCCGGCACCGACGCCACCCGCGTCGCGAGCTACAACCCCTGGGTGTCGCTGT
CGTGGCTGGTGACCGGCAGGACGGTGGGCGGCCTGCAGATCACGCCGCAGCGCAACTGCCTCACCCGTGACCAGGCGCTG
CGCATGTGGACCGAGAACGTCACCTGGTTCAGCAACGAGCAGGGCAGGAAGGGCCGCATCCAGGAGGGCCAGCTGGCCGA
CCTGGTCGTGCCCGACCGCGACTTCTTCGCCTGCGCCGAATCCGAGATCGCCGACACCACGGCCCTGCTCACGATGGTGG
CTGGCAAGGTGGTCTATGGCGCGGGCGATTTCGCCCGCTTCGACGAAGGCGCGCCGCCGCCGGCCATGCCCGACTGGTCG
CCCGTGCGCACCTTCCGCGGCTATGGCGCGTGGGGCGTTCAGGAGGGTGCGCCGCTGCAGGCGGTGATGCGCAACGCGGC
GGCGAGCTGCGGCTGCGCCAGCCAGTGCAACCTGCACGGCCACGCGCATGCCACGGCCTGGAGCAGCCGGCTGCCCGTGT
CCGACCTGAAGGGCTTCTGGGGCGCGCTGGGCTGCGCCTGCTGGGCGGTGTGA

Upstream 100 bases:

>100_bases
ACGGCCTGGGGATCACTTACGCCAAGACCATGTTCAACGCCCACGAAGGCTGAAAAAAAGGCGCTCTGCCATCGTCAAAC
CCCTTGAAGGCCTGAACACC

Downstream 100 bases:

>100_bases
CGTGGACGGGATGCTGCATGCCGCGGCCGCCGCCTCCTGGGTGCAGCCGCTGGCCCTGCTGTGCCTGTGCGCGGCCTACC
TGCAGGGTGGATGGGACAAG

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTTPATTADLILRNGRFTTLDRANPTASAVAIAQGRFTRVGGDREVMALAGPQTRVIDLGGRSVLPGLIDNHLHIIRGGL
NFNMELRWDGVKSLADAMAMLKRQVDVTPAPQWVRVVGGFTEHQFVEKRLPTLAELNAVAPDTPVFILHLYDRALLNGAA
LRAVGYGRDTPAPPGGEIVRDSAGNPTGLLLAKPNAAILYATLAKGPKLPHEYQVNSTRHFMRELNRLGVTGAIDAGGGM
QNYPDDYQVIQELADAGQLTIRLAYNLFTQKPKEEKADFLHWTATSQYKQGTDYFRHNGAGEMLVFSAADFEDFRQPRPE
LAPEMEGELEEVVRILAQNRWPWRMHATYDETIDRALGVFEKVNRDTPLAGLNWFFDHAETISEKSIDRIAALGGGVAVQ
HRMAYQGEYFMERYGAAAAEATPPVKRMLEKGVQVSAGTDATRVASYNPWVSLSWLVTGRTVGGLQITPQRNCLTRDQAL
RMWTENVTWFSNEQGRKGRIQEGQLADLVVPDRDFFACAESEIADTTALLTMVAGKVVYGAGDFARFDEGAPPPAMPDWS
PVRTFRGYGAWGVQEGAPLQAVMRNAAASCGCASQCNLHGHAHATAWSSRLPVSDLKGFWGALGCACWAV

Sequences:

>Translated_630_residues
MTTPATTADLILRNGRFTTLDRANPTASAVAIAQGRFTRVGGDREVMALAGPQTRVIDLGGRSVLPGLIDNHLHIIRGGL
NFNMELRWDGVKSLADAMAMLKRQVDVTPAPQWVRVVGGFTEHQFVEKRLPTLAELNAVAPDTPVFILHLYDRALLNGAA
LRAVGYGRDTPAPPGGEIVRDSAGNPTGLLLAKPNAAILYATLAKGPKLPHEYQVNSTRHFMRELNRLGVTGAIDAGGGM
QNYPDDYQVIQELADAGQLTIRLAYNLFTQKPKEEKADFLHWTATSQYKQGTDYFRHNGAGEMLVFSAADFEDFRQPRPE
LAPEMEGELEEVVRILAQNRWPWRMHATYDETIDRALGVFEKVNRDTPLAGLNWFFDHAETISEKSIDRIAALGGGVAVQ
HRMAYQGEYFMERYGAAAAEATPPVKRMLEKGVQVSAGTDATRVASYNPWVSLSWLVTGRTVGGLQITPQRNCLTRDQAL
RMWTENVTWFSNEQGRKGRIQEGQLADLVVPDRDFFACAESEIADTTALLTMVAGKVVYGAGDFARFDEGAPPPAMPDWS
PVRTFRGYGAWGVQEGAPLQAVMRNAAASCGCASQCNLHGHAHATAWSSRLPVSDLKGFWGALGCACWAV
>Mature_629_residues
TTPATTADLILRNGRFTTLDRANPTASAVAIAQGRFTRVGGDREVMALAGPQTRVIDLGGRSVLPGLIDNHLHIIRGGLN
FNMELRWDGVKSLADAMAMLKRQVDVTPAPQWVRVVGGFTEHQFVEKRLPTLAELNAVAPDTPVFILHLYDRALLNGAAL
RAVGYGRDTPAPPGGEIVRDSAGNPTGLLLAKPNAAILYATLAKGPKLPHEYQVNSTRHFMRELNRLGVTGAIDAGGGMQ
NYPDDYQVIQELADAGQLTIRLAYNLFTQKPKEEKADFLHWTATSQYKQGTDYFRHNGAGEMLVFSAADFEDFRQPRPEL
APEMEGELEEVVRILAQNRWPWRMHATYDETIDRALGVFEKVNRDTPLAGLNWFFDHAETISEKSIDRIAALGGGVAVQH
RMAYQGEYFMERYGAAAAEATPPVKRMLEKGVQVSAGTDATRVASYNPWVSLSWLVTGRTVGGLQITPQRNCLTRDQALR
MWTENVTWFSNEQGRKGRIQEGQLADLVVPDRDFFACAESEIADTTALLTMVAGKVVYGAGDFARFDEGAPPPAMPDWSP
VRTFRGYGAWGVQEGAPLQAVMRNAAASCGCASQCNLHGHAHATAWSSRLPVSDLKGFWGALGCACWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69009; Mature: 68878

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPATTADLILRNGRFTTLDRANPTASAVAIAQGRFTRVGGDREVMALAGPQTRVIDLG
CCCCCCCHHHEEECCCEEEECCCCCCHHEEEEECCCEEECCCCCEEEEEECCCEEEEECC
GRSVLPGLIDNHLHIIRGGLNFNMELRWDGVKSLADAMAMLKRQVDVTPAPQWVRVVGGF
CCCCCCHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TEHQFVEKRLPTLAELNAVAPDTPVFILHLYDRALLNGAALRAVGYGRDTPAPPGGEIVR
HHHHHHHHHCCHHHHHCCCCCCCCEEEEEEHHHHHHCCHHHEEECCCCCCCCCCCCCEEE
DSAGNPTGLLLAKPNAAILYATLAKGPKLPHEYQVNSTRHFMRELNRLGVTGAIDAGGGM
CCCCCCCEEEEECCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCEEECCCCCC
QNYPDDYQVIQELADAGQLTIRLAYNLFTQKPKEEKADFLHWTATSQYKQGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHCCEEEEECCCCHHCCCHHHHHCCC
GEMLVFSAADFEDFRQPRPELAPEMEGELEEVVRILAQNRWPWRMHATYDETIDRALGVF
CCEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNRDTPLAGLNWFFDHAETISEKSIDRIAALGGGVAVQHRMAYQGEYFMERYGAAAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCCCCC
ATPPVKRMLEKGVQVSAGTDATRVASYNPWVSLSWLVTGRTVGGLQITPQRNCLTRDQAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCHHHHHHHH
RMWTENVTWFSNEQGRKGRIQEGQLADLVVPDRDFFACAESEIADTTALLTMVAGKVVYG
HHHHCCCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHEEC
AGDFARFDEGAPPPAMPDWSPVRTFRGYGAWGVQEGAPLQAVMRNAAASCGCASQCNLHG
CCCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
HAHATAWSSRLPVSDLKGFWGALGCACWAV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPATTADLILRNGRFTTLDRANPTASAVAIAQGRFTRVGGDREVMALAGPQTRVIDLG
CCCCCCHHHEEECCCEEEECCCCCCHHEEEEECCCEEECCCCCEEEEEECCCEEEEECC
GRSVLPGLIDNHLHIIRGGLNFNMELRWDGVKSLADAMAMLKRQVDVTPAPQWVRVVGGF
CCCCCCHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TEHQFVEKRLPTLAELNAVAPDTPVFILHLYDRALLNGAALRAVGYGRDTPAPPGGEIVR
HHHHHHHHHCCHHHHHCCCCCCCCEEEEEEHHHHHHCCHHHEEECCCCCCCCCCCCCEEE
DSAGNPTGLLLAKPNAAILYATLAKGPKLPHEYQVNSTRHFMRELNRLGVTGAIDAGGGM
CCCCCCCEEEEECCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCEEECCCCCC
QNYPDDYQVIQELADAGQLTIRLAYNLFTQKPKEEKADFLHWTATSQYKQGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHCCEEEEECCCCHHCCCHHHHHCCC
GEMLVFSAADFEDFRQPRPELAPEMEGELEEVVRILAQNRWPWRMHATYDETIDRALGVF
CCEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNRDTPLAGLNWFFDHAETISEKSIDRIAALGGGVAVQHRMAYQGEYFMERYGAAAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCCCCC
ATPPVKRMLEKGVQVSAGTDATRVASYNPWVSLSWLVTGRTVGGLQITPQRNCLTRDQAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCEECCEEECCCCCHHHHHHHH
RMWTENVTWFSNEQGRKGRIQEGQLADLVVPDRDFFACAESEIADTTALLTMVAGKVVYG
HHHHCCCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHEEC
AGDFARFDEGAPPPAMPDWSPVRTFRGYGAWGVQEGAPLQAVMRNAAASCGCASQCNLHG
CCCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
HAHATAWSSRLPVSDLKGFWGALGCACWAV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA