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The map label for this gene is narY [H]

Identifier: 120609361

GI number: 120609361

Start: 720408

End: 721931

Strand: Direct

Name: narY [H]

Synonym: Aave_0662

Alternate gene names: 120609361

Gene position: 720408-721931 (Clockwise)

Preceding gene: 120609360

Following gene: 120609362

Centisome position: 13.46

GC content: 66.34

Gene sequence:

>1524_bases
ATGAGAGTCCGCGCACAGATCGGCATGGTGCTCAACCTGGACAAGTGCATCGGCTGCCACACCTGCTCCGTCACCTGCAA
GAACGTCTGGACCAGCCGCCCGGGCATGGAATACGCCTGGTTCAACAACGTCGAGACCAAGCCCGGCGTCGGCTACCCCA
AGGACTGGGAGAACCAGGACCGCTGGAACGGCGGCTGGGTGCGGCGCGCGGACGGCTCCATCGCGCCGCGCCAGGGCGCG
AAGTGGAAGCTTTTGATGAAGATCTTCGCCAACCCCAACCTGCCGGAGATCGACGACTACTACGAACCCTTCACCTACGA
CTACGCGCACCTGCAGTCCGCGCCCGAGATGAAGGCCGCGCCCACCGCGCGCCCGCGCAGCCTGATCACGGGCAGGCAGA
TGGACAAGATCGTCTGGGGCCCGAACTGGGAAGAGATCCTGGGCGGCGAATTCTCCAAGCGCAGCCGCGACAGGAACTTC
GAGGACGTGCAGAAGGACATGTACGCGCAGTTCGAGAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAGCACTGCCT
CAATCCGGCGTGCGTGGCCTCGTGCCCCTCGGGCTCGATCTACAAGCGCGAGGAAGACGGCATCGTCCTCATCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCGTCTCGGGCTGCCCCTACAAGAAGATCTACTACAACTGGAAGAGCGGCAAGGCC
GAGAAGTGCATCTTCTGCTATCCGCGCATCGAGTCGGGCCAGCCCACCGTGTGCTCGGAGACCTGCGTGGGCCGCATCCG
CTACCTGGGCGTGCTGCTCTACGACGCCGACCGCATCGCCGAGGCCGCGAGCGTGGAGCGCGACCGCGACCTGTACCGCG
CGCAGCTCGACATCTTCCTGGACCCGAACGACCCCGAGGTGATCGCGCAGGCCCGCATCGACGGCATTCCCGACGCCTGG
ATGGAGGCCGCGCGCAGGAGCCCTGTTTACAAGATGGCGGTGGACTGGAAGGTGGCCCTGCCGCTGCACCCCGAGTACCG
CACGCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCGCCCATCAGCGCCGCGGCCAACGCGGGGCACGTGGGTGCCAACG
GCCTCATCCCGGACGTGGGCCAGCTGCGCATTCCCGTGCAGTACCTCGCCAACCTGCTCACGGCCGGCGACACCGCTCCG
GTGGTGCGCGCGCTCGAACGCATGCTCGCCATGCGCGCCTTCCAGCGTGCGAAGCACGTGGAGCAGCGCACCGACCTCGA
CGTGCTCGCGCAGGTGGGCCTCACGCAGGAGGAGGTGGAGGACATGTACCAGACCATGGCCATCGCCAACTACGAAGACC
GTTTCGTCATCCCCACCACGCACCGCGAGTATGCGGAGAACGCCTTCAACGTGCGCGGCGGCTGCGGCTTCAGCTTCGGC
AACGGCTGCTCCGACGGCGTGAGCGACGTGAGCCTGTTCGGTGCCGAGAAGAAGCGCACCATCCCCATCAAGGCGGAAGT
CTGA

Upstream 100 bases:

>100_bases
ACGAATTCATCGTGCTGCGCAAGATGAACCGCGTGGACTGGCTCGACACGCCCGTCGCCGACCCGCTCGTCCGCCCCACC
CTGGACCAAGGAGAAACCGC

Downstream 100 bases:

>100_bases
CATGGGCGCCACCACCGACCTCCGCACTGCCCGGGCGGCCATCGCCGGCCGCCATCCCGGCAGCGCCACGCTGCGCGTCT
TGGGCGCGCTGCTCGACTAC

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGVGYPKDWENQDRWNGGWVRRADGSIAPRQGA
KWKLLMKIFANPNLPEIDDYYEPFTYDYAHLQSAPEMKAAPTARPRSLITGRQMDKIVWGPNWEEILGGEFSKRSRDRNF
EDVQKDMYAQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIAEAASVERDRDLYRAQLDIFLDPNDPEVIAQARIDGIPDAW
MEAARRSPVYKMAVDWKVALPLHPEYRTLPMVWYVPPLSPISAAANAGHVGANGLIPDVGQLRIPVQYLANLLTAGDTAP
VVRALERMLAMRAFQRAKHVEQRTDLDVLAQVGLTQEEVEDMYQTMAIANYEDRFVIPTTHREYAENAFNVRGGCGFSFG
NGCSDGVSDVSLFGAEKKRTIPIKAEV

Sequences:

>Translated_507_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGVGYPKDWENQDRWNGGWVRRADGSIAPRQGA
KWKLLMKIFANPNLPEIDDYYEPFTYDYAHLQSAPEMKAAPTARPRSLITGRQMDKIVWGPNWEEILGGEFSKRSRDRNF
EDVQKDMYAQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIAEAASVERDRDLYRAQLDIFLDPNDPEVIAQARIDGIPDAW
MEAARRSPVYKMAVDWKVALPLHPEYRTLPMVWYVPPLSPISAAANAGHVGANGLIPDVGQLRIPVQYLANLLTAGDTAP
VVRALERMLAMRAFQRAKHVEQRTDLDVLAQVGLTQEEVEDMYQTMAIANYEDRFVIPTTHREYAENAFNVRGGCGFSFG
NGCSDGVSDVSLFGAEKKRTIPIKAEV
>Mature_507_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGVGYPKDWENQDRWNGGWVRRADGSIAPRQGA
KWKLLMKIFANPNLPEIDDYYEPFTYDYAHLQSAPEMKAAPTARPRSLITGRQMDKIVWGPNWEEILGGEFSKRSRDRNF
EDVQKDMYAQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIAEAASVERDRDLYRAQLDIFLDPNDPEVIAQARIDGIPDAW
MEAARRSPVYKMAVDWKVALPLHPEYRTLPMVWYVPPLSPISAAANAGHVGANGLIPDVGQLRIPVQYLANLLTAGDTAP
VVRALERMLAMRAFQRAKHVEQRTDLDVLAQVGLTQEEVEDMYQTMAIANYEDRFVIPTTHREYAENAFNVRGGCGFSFG
NGCSDGVSDVSLFGAEKKRTIPIKAEV

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=504, Percent_Identity=71.4285714285714, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=508, Percent_Identity=69.488188976378, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=158, Percent_Identity=35.4430379746835, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787122, Length=142, Percent_Identity=35.9154929577465, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI2367345, Length=142, Percent_Identity=26.056338028169, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789370, Length=106, Percent_Identity=37.7358490566038, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=34.4444444444444, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57441; Mature: 57441

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGVGYPKDWENQD
CCCCEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
RWNGGWVRRADGSIAPRQGAKWKLLMKIFANPNLPEIDDYYEPFTYDYAHLQSAPEMKAA
CCCCCEEEECCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGRQMDKIVWGPNWEEILGGEFSKRSRDRNFEDVQKDMYAQFENTFMMYLP
CCCCCHHHHCCCCCCCEEECCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIAEAASVERDRDLYRAQLDIFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHEEEEEEEEE
DPNDPEVIAQARIDGIPDAWMEAARRSPVYKMAVDWKVALPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
ISAAANAGHVGANGLIPDVGQLRIPVQYLANLLTAGDTAPVVRALERMLAMRAFQRAKHV
HHHHCCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EQRTDLDVLAQVGLTQEEVEDMYQTMAIANYEDRFVIPTTHREYAENAFNVRGGCGFSFG
HHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCC
NGCSDGVSDVSLFGAEKKRTIPIKAEV
CCCCCCHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGVGYPKDWENQD
CCCCEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
RWNGGWVRRADGSIAPRQGAKWKLLMKIFANPNLPEIDDYYEPFTYDYAHLQSAPEMKAA
CCCCCEEEECCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGRQMDKIVWGPNWEEILGGEFSKRSRDRNFEDVQKDMYAQFENTFMMYLP
CCCCCHHHHCCCCCCCEEECCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIAEAASVERDRDLYRAQLDIFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHEEEEEEEEE
DPNDPEVIAQARIDGIPDAWMEAARRSPVYKMAVDWKVALPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
ISAAANAGHVGANGLIPDVGQLRIPVQYLANLLTAGDTAPVVRALERMLAMRAFQRAKHV
HHHHCCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EQRTDLDVLAQVGLTQEEVEDMYQTMAIANYEDRFVIPTTHREYAENAFNVRGGCGFSFG
HHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCC
NGCSDGVSDVSLFGAEKKRTIPIKAEV
CCCCCCHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]