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The map label for this gene is topB [H]

Identifier: 120609259

GI number: 120609259

Start: 604492

End: 606534

Strand: Reverse

Name: topB [H]

Synonym: Aave_0558

Alternate gene names: 120609259

Gene position: 606534-604492 (Counterclockwise)

Preceding gene: 120609260

Following gene: 120609258

Centisome position: 11.33

GC content: 67.16

Gene sequence:

>2043_bases
ATGCGCGTTGTTCTGTGCGAGAAGCCCTCCCAAGGCAAGGACATCGCGAGGGTCCTGGGCGCACGCCAGCGTGAAGCGGG
CTGCCTGGCCGGGCCGGGCATCAAGGTCACCTGGGGCATCGGCCACCTGATCGAGGCTGCGCCCCCGGAGGCCTACGGCG
AGCACTTTCAGCGCTGGTCGCTGGAGACGCTGCCGATCGTTCCACAGCAGTGGCGGTGGGTGGTCAAGGCCGACACCGCC
TCGCAATTCAAGGTCGTCCAGCGCCTGCTGGCACAGGCGTCCGAACTCATCATTGCCACGGACGCCGATCGCGAGGGCGA
GATGATCGCCCGCGAGTTGATCGAGTACTGCGGATACCGCGGACCCATACAACGGCTATGGCTCTCCGCGCTCAACGATG
CGTCGATCCGCAAAGCGCTGGGCGCCCTGCGGGCATCGCACGAAACGCTTCCGCTGTACCACTGCGCGCTCGCGCGCTCC
CATGCGGACTGGCTCGTCGGCATCAATCTCACGCGCCTGTTCACGCTGCTGGGGCGCAAGGCCGGTTTCGACGGGGTGCT
TTCGGTGGGCCGCGTGCAGACGCCCACCCTCAAGCTGGTCGCTGACCGCGATCGGGAGATTGCGGCGTTCGTTCCGGTCC
CGTTCTGGTCCATCGAGGTCTCGCTCTCGGCGGGCGCGCAGGCGTTCTCCGCACACTGGGTTCCACCTGCAACCGTCACG
GACGCTGCAGGCCGGTGCTTACAGCAGAGCGCGGCTCAACACGCGGCGGCCGCCGTCCGCACGGCCGGGCACGCGCAGGT
CACGGGCATTGATACCGAGCGTGTGCAGGAAGGCCCTCCGCTTCCGTTCGACCTCAGCACGCTGCAGGAAGTCTGTTCAC
GGCGTCTCGGCCTCGACGTGCAGGAGACGCTCGACATCGCCCAGGCCTTGTACGAGACGCACAAAGCGACGACGTATCCG
CGCACCGACGCGGGCTATCTGCCAGAGAACATGCTCACCGAGGTTCCCACCGTCATCCGGAGCCTGTTGAAGACGGACCC
GGCGCTGCAACCGCTCATGCAGCGCCTCGACCTGGGTCAGCGCTCCCGCGCCTGGAACGCCACCAAGATCGCGGCCCACC
ACGCCATCATCCCGACGCTGGAGCCTGCCGACCTGTCGAAGATGTCCGAGAAGGAACTGGCCGTGTACCGGCTCATCCGC
GCCCACTACCTGGCCCAGTTCCTTCCCCACTATGCCTATGATCGAACGATGGTGCGCCTCGCATGCGGCGGCCAAGCGCT
GCAGGCGTCCGGGAAGCAGGTCGTCGATGCGGGCTGGAAGGCCGCGTTCGCTGCTCCCTCGATCCCCGGTGCAGGGGCGG
AGACTCCCGAAGCCGAGACGGACAGCGAAGCTCCGCGCGACCAGGCGCTGCCGACACTGCACGAAGGGATGGCCTGCGAG
GTCAGGTCGCTCGATCTGAAGTCGCTGTACACCAAGTCTCCGCCCCCCTACACCCAGGGCGAGCTCGTCAAGGCGATGAA
GACCGTCGCCAAGCTCGTCACCGATCCGCGACTGAAGCAAACGCTGAGGGACACCACGGGCATCGGAACGGAGGCGACGC
GCGCGAACATCATCAGCGGGTTGCTCGCGCGCGGGTTCCTGGTGCGCAAGGGCCGCGTGGTTCGCGCATCCAAGACCGCG
ATGACGCTCATCGACGCAGTTCCCGCAGCGATTGCGGACCCCGGCACCACGGCAATATGGGAGCAGGCGCTGCACATGAT
CGAGGCGCGCACGATGGACCTCGACACGTTCGTCTCGAAGCAGGCCGCATGGGTTTCGCAGCTGGTCGAACAGCATCGCG
AAGCGACCCTCGCGATCCGCGTTCCTGCGGGTCCGCCATGCCCGCTCTGCGGTGCCGCAACGCGCAAGCGCACCGGCAAA
ACCGGAGCCTTCTGGTCCTGCTCCCGCTATCCTGACTGCAAGGGTACCGTTGCGATCGATTCCGCCGAAGCGGGCCGGCG
CAACGGGCCGCGCAAGCCGCGCGCCGCCCGAAAGCTTCACTAG

Upstream 100 bases:

>100_bases
GAGCGTGCGGCGCGCACCTTCTTCGCTGCGCCAGCCCACTGGGCCCGCGATGCTGACGGCCATCTGATGACCGTCTTGCA
TTGCTTGGAGGGCCTCTCTC

Downstream 100 bases:

>100_bases
TCTCGGCGCCCCACCCCAAACACTCCGACCGCACCGCAGGTGCGGCCGCCTCGCGCCCCGCGAGGCCCCAGCATGCCCCG
CCTTCTGCGAAGCGTGCCCG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MRVVLCEKPSQGKDIARVLGARQREAGCLAGPGIKVTWGIGHLIEAAPPEAYGEHFQRWSLETLPIVPQQWRWVVKADTA
SQFKVVQRLLAQASELIIATDADREGEMIARELIEYCGYRGPIQRLWLSALNDASIRKALGALRASHETLPLYHCALARS
HADWLVGINLTRLFTLLGRKAGFDGVLSVGRVQTPTLKLVADRDREIAAFVPVPFWSIEVSLSAGAQAFSAHWVPPATVT
DAAGRCLQQSAAQHAAAAVRTAGHAQVTGIDTERVQEGPPLPFDLSTLQEVCSRRLGLDVQETLDIAQALYETHKATTYP
RTDAGYLPENMLTEVPTVIRSLLKTDPALQPLMQRLDLGQRSRAWNATKIAAHHAIIPTLEPADLSKMSEKELAVYRLIR
AHYLAQFLPHYAYDRTMVRLACGGQALQASGKQVVDAGWKAAFAAPSIPGAGAETPEAETDSEAPRDQALPTLHEGMACE
VRSLDLKSLYTKSPPPYTQGELVKAMKTVAKLVTDPRLKQTLRDTTGIGTEATRANIISGLLARGFLVRKGRVVRASKTA
MTLIDAVPAAIADPGTTAIWEQALHMIEARTMDLDTFVSKQAAWVSQLVEQHREATLAIRVPAGPPCPLCGAATRKRTGK
TGAFWSCSRYPDCKGTVAIDSAEAGRRNGPRKPRAARKLH

Sequences:

>Translated_680_residues
MRVVLCEKPSQGKDIARVLGARQREAGCLAGPGIKVTWGIGHLIEAAPPEAYGEHFQRWSLETLPIVPQQWRWVVKADTA
SQFKVVQRLLAQASELIIATDADREGEMIARELIEYCGYRGPIQRLWLSALNDASIRKALGALRASHETLPLYHCALARS
HADWLVGINLTRLFTLLGRKAGFDGVLSVGRVQTPTLKLVADRDREIAAFVPVPFWSIEVSLSAGAQAFSAHWVPPATVT
DAAGRCLQQSAAQHAAAAVRTAGHAQVTGIDTERVQEGPPLPFDLSTLQEVCSRRLGLDVQETLDIAQALYETHKATTYP
RTDAGYLPENMLTEVPTVIRSLLKTDPALQPLMQRLDLGQRSRAWNATKIAAHHAIIPTLEPADLSKMSEKELAVYRLIR
AHYLAQFLPHYAYDRTMVRLACGGQALQASGKQVVDAGWKAAFAAPSIPGAGAETPEAETDSEAPRDQALPTLHEGMACE
VRSLDLKSLYTKSPPPYTQGELVKAMKTVAKLVTDPRLKQTLRDTTGIGTEATRANIISGLLARGFLVRKGRVVRASKTA
MTLIDAVPAAIADPGTTAIWEQALHMIEARTMDLDTFVSKQAAWVSQLVEQHREATLAIRVPAGPPCPLCGAATRKRTGK
TGAFWSCSRYPDCKGTVAIDSAEAGRRNGPRKPRAARKLH
>Mature_680_residues
MRVVLCEKPSQGKDIARVLGARQREAGCLAGPGIKVTWGIGHLIEAAPPEAYGEHFQRWSLETLPIVPQQWRWVVKADTA
SQFKVVQRLLAQASELIIATDADREGEMIARELIEYCGYRGPIQRLWLSALNDASIRKALGALRASHETLPLYHCALARS
HADWLVGINLTRLFTLLGRKAGFDGVLSVGRVQTPTLKLVADRDREIAAFVPVPFWSIEVSLSAGAQAFSAHWVPPATVT
DAAGRCLQQSAAQHAAAAVRTAGHAQVTGIDTERVQEGPPLPFDLSTLQEVCSRRLGLDVQETLDIAQALYETHKATTYP
RTDAGYLPENMLTEVPTVIRSLLKTDPALQPLMQRLDLGQRSRAWNATKIAAHHAIIPTLEPADLSKMSEKELAVYRLIR
AHYLAQFLPHYAYDRTMVRLACGGQALQASGKQVVDAGWKAAFAAPSIPGAGAETPEAETDSEAPRDQALPTLHEGMACE
VRSLDLKSLYTKSPPPYTQGELVKAMKTVAKLVTDPRLKQTLRDTTGIGTEATRANIISGLLARGFLVRKGRVVRASKTA
MTLIDAVPAAIADPGTTAIWEQALHMIEARTMDLDTFVSKQAAWVSQLVEQHREATLAIRVPAGPPCPLCGAATRKRTGK
TGAFWSCSRYPDCKGTVAIDSAEAGRRNGPRKPRAARKLH

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=685, Percent_Identity=21.7518248175182, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI10835218, Length=658, Percent_Identity=22.9483282674772, Blast_Score=100, Evalue=8e-21,
Organism=Escherichia coli, GI1788061, Length=658, Percent_Identity=36.9300911854103, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1787529, Length=596, Percent_Identity=26.1744966442953, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=576, Percent_Identity=24.6527777777778, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=641, Percent_Identity=21.996879875195, Blast_Score=113, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=522, Percent_Identity=26.0536398467433, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=24.1112828438949, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640096, Length=489, Percent_Identity=22.9038854805726, Blast_Score=103, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74020; Mature: 74020

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVLCEKPSQGKDIARVLGARQREAGCLAGPGIKVTWGIGHLIEAAPPEAYGEHFQRWS
CEEEEECCCCCCHHHHHHHCCCHHCCCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHCC
LETLPIVPQQWRWVVKADTASQFKVVQRLLAQASELIIATDADREGEMIARELIEYCGYR
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCC
GPIQRLWLSALNDASIRKALGALRASHETLPLYHCALARSHADWLVGINLTRLFTLLGRK
CHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHCC
AGFDGVLSVGRVQTPTLKLVADRDREIAAFVPVPFWSIEVSLSAGAQAFSAHWVPPATVT
CCCHHHHHCCCCCCCEEEEEECCCCCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCHHH
DAAGRCLQQSAAQHAAAAVRTAGHAQVTGIDTERVQEGPPLPFDLSTLQEVCSRRLGLDV
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRTDAGYLPENMLTEVPTVIRSLLKTDPALQPLMQRLDLGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
RSRAWNATKIAAHHAIIPTLEPADLSKMSEKELAVYRLIRAHYLAQFLPHYAYDRTMVRL
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
ACGGQALQASGKQVVDAGWKAAFAAPSIPGAGAETPEAETDSEAPRDQALPTLHEGMACE
ECCCHHHHHCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCEEE
VRSLDLKSLYTKSPPPYTQGELVKAMKTVAKLVTDPRLKQTLRDTTGIGTEATRANIISG
HHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LLARGFLVRKGRVVRASKTAMTLIDAVPAAIADPGTTAIWEQALHMIEARTMDLDTFVSK
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
QAAWVSQLVEQHREATLAIRVPAGPPCPLCGAATRKRTGKTGAFWSCSRYPDCKGTVAID
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCEECCCCCCCCCEEEEC
SAEAGRRNGPRKPRAARKLH
CCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MRVVLCEKPSQGKDIARVLGARQREAGCLAGPGIKVTWGIGHLIEAAPPEAYGEHFQRWS
CEEEEECCCCCCHHHHHHHCCCHHCCCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHCC
LETLPIVPQQWRWVVKADTASQFKVVQRLLAQASELIIATDADREGEMIARELIEYCGYR
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHCCCC
GPIQRLWLSALNDASIRKALGALRASHETLPLYHCALARSHADWLVGINLTRLFTLLGRK
CHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHCC
AGFDGVLSVGRVQTPTLKLVADRDREIAAFVPVPFWSIEVSLSAGAQAFSAHWVPPATVT
CCCHHHHHCCCCCCCEEEEEECCCCCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCHHH
DAAGRCLQQSAAQHAAAAVRTAGHAQVTGIDTERVQEGPPLPFDLSTLQEVCSRRLGLDV
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETHKATTYPRTDAGYLPENMLTEVPTVIRSLLKTDPALQPLMQRLDLGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
RSRAWNATKIAAHHAIIPTLEPADLSKMSEKELAVYRLIRAHYLAQFLPHYAYDRTMVRL
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
ACGGQALQASGKQVVDAGWKAAFAAPSIPGAGAETPEAETDSEAPRDQALPTLHEGMACE
ECCCHHHHHCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCEEE
VRSLDLKSLYTKSPPPYTQGELVKAMKTVAKLVTDPRLKQTLRDTTGIGTEATRANIISG
HHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LLARGFLVRKGRVVRASKTAMTLIDAVPAAIADPGTTAIWEQALHMIEARTMDLDTFVSK
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
QAAWVSQLVEQHREATLAIRVPAGPPCPLCGAATRKRTGKTGAFWSCSRYPDCKGTVAID
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCEECCCCCCCCCEEEEC
SAEAGRRNGPRKPRAARKLH
CCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]