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The map label for this gene is oprM [H]

Identifier: 120609090

GI number: 120609090

Start: 414089

End: 415567

Strand: Reverse

Name: oprM [H]

Synonym: Aave_0387

Alternate gene names: 120609090

Gene position: 415567-414089 (Counterclockwise)

Preceding gene: 120609091

Following gene: 120609089

Centisome position: 7.76

GC content: 71.87

Gene sequence:

>1479_bases
ATGCAAGCACCCTCCCGCGCACGCCTCGGCGCGCGCCCCCTGCTCTGCGCAGCCGCCGCCGCGGCGCTGCTCGCCGGCTG
CAGCTTCATCCCCACCTACGAACGCCCGGCGGCGCCGGTGCCCCAGGCCTACCCGGCCGCCGGCGCCGCCGCGGCGCCGG
CCGGCGCACGGGCCGCGGCGGACATCGACTGGCGCGAGTATTTCGCCGATCCGCGGCTGCAGCGCCTGATCGCGCTGGCG
CTGGAGAACAACCGCGACCTGCGCGTGGCCGCGCTCAACATCGAGCAGGCCCGTGCGCAGTTCCAGATCCAGCGCGCGGG
GCAGTTCCCCACGGTGAACGCCGCGGTGAACGCATCGCGCCAGCCGAGCGCCACCACGGGCAGCTACGTCAACAACTACC
AGGTGGGACTGGCGGTCTCTGCCTGGGAAATCGACTTCTTCGGCCGCATCGCGGCGCTCAAGGAGCAGGCCCTGGCGCAG
TATTTCGCGACCGAGGAAGCCCGCCGCGCCACGCAGATCAGCCTGGTCGCCGCCGTGGCGTCGGGCTGGTTCAACCTGCT
CGCCGACGAGGAACTGCTCGACATATCGCGCCGCACCCTGCAGACGCGCGACGAATCCGTGCAGCTCACCAGGCTGCGGC
TCGAGAACGGCGTGAGCTCCGAACTCGATTACAGCCAGGCCCAGGGCCTGGCCCAGGCCGCGCGCGCCACCTTCGCGCAG
CAGCAGCGCCAGCGCCTGCAGGACGAGAACGCGCTCGCTTTGCTGCTGGGCCAGCCGCTGCCGCAGGAGATCGCCGCCAC
GCTCGATGCGCGCACGCGCCTGGCCGACGCACCCGCCATGCCGTCGCTGCCCGCCGGCCTGCCTTCGGACCTGCTGGTAC
GCCGCCCCGACATCCGCCAGGCGGAGCAGCAGCTCGTGGCTGCCAACGCCAACATCGGCGCGGCCCGGGCCGCGTTCTTT
CCGCGCATCTCGCTCACGGCCAGCGCCGGCACCGTCAGCAACGAACTGTCGGGTCTGTTCAAGGGCGGCTCCTGGGCCTT
TTCGCTGGCACCGCAACTGGCGCTGCCGATCTTCGACGGCGGCCGCAACCAGGCCACGCTGGATTCCGCACGCGTGGGCC
GCGACATCGCCGTGGCTCAGTACGAAAAATCGATCCAGACGGCCTTCCGCGAAGTGTCGGACGCGCTGGCGGGGCAGGCC
ACGCTGGGCGAGCAGTTCGCGGCGCAGCGCGCACAGGCCGCGGCCGATGCCAAGCGCTTCGAGTTGTCCGACCTGCGCTA
CCGCAACGGCGTGGCGAGCTACCTCGACCTGCTGGACGCACAGCGCTCGCTGTTCACCTCCGAGCAACTGGTGGTGCAGA
CGCGGCTGCTGCAGCTGCAGAATCAGGTCGCACTCTACAAAGTGCTCGGCGGCGGCTGGACGGCAGCCCAGGAGCCCCCG
GCGACGGGCGGCGGCGCCACCGCAGCGCCGCACTCCTGA

Upstream 100 bases:

>100_bases
AGCAAGCGCCAGCACGACCACGAGGCACGCCAGGCGCATGCCCACCGGCATGCCACGGATGGGGAATAGGCCCGACCAAG
AACGGCAGGAGAAGAACACC

Downstream 100 bases:

>100_bases
GCCGGCCTTCCGCTCTTTTCCAGGCGCCCTCGGGCGCCTTTTTCCTGTGCACCGGGTCTGCCTGCCGAAGCCATGCCCGG
CACCTCCAACGGATGCGAAT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MQAPSRARLGARPLLCAAAAAALLAGCSFIPTYERPAAPVPQAYPAAGAAAAPAGARAAADIDWREYFADPRLQRLIALA
LENNRDLRVAALNIEQARAQFQIQRAGQFPTVNAAVNASRQPSATTGSYVNNYQVGLAVSAWEIDFFGRIAALKEQALAQ
YFATEEARRATQISLVAAVASGWFNLLADEELLDISRRTLQTRDESVQLTRLRLENGVSSELDYSQAQGLAQAARATFAQ
QQRQRLQDENALALLLGQPLPQEIAATLDARTRLADAPAMPSLPAGLPSDLLVRRPDIRQAEQQLVAANANIGAARAAFF
PRISLTASAGTVSNELSGLFKGGSWAFSLAPQLALPIFDGGRNQATLDSARVGRDIAVAQYEKSIQTAFREVSDALAGQA
TLGEQFAAQRAQAAADAKRFELSDLRYRNGVASYLDLLDAQRSLFTSEQLVVQTRLLQLQNQVALYKVLGGGWTAAQEPP
ATGGGATAAPHS

Sequences:

>Translated_492_residues
MQAPSRARLGARPLLCAAAAAALLAGCSFIPTYERPAAPVPQAYPAAGAAAAPAGARAAADIDWREYFADPRLQRLIALA
LENNRDLRVAALNIEQARAQFQIQRAGQFPTVNAAVNASRQPSATTGSYVNNYQVGLAVSAWEIDFFGRIAALKEQALAQ
YFATEEARRATQISLVAAVASGWFNLLADEELLDISRRTLQTRDESVQLTRLRLENGVSSELDYSQAQGLAQAARATFAQ
QQRQRLQDENALALLLGQPLPQEIAATLDARTRLADAPAMPSLPAGLPSDLLVRRPDIRQAEQQLVAANANIGAARAAFF
PRISLTASAGTVSNELSGLFKGGSWAFSLAPQLALPIFDGGRNQATLDSARVGRDIAVAQYEKSIQTAFREVSDALAGQA
TLGEQFAAQRAQAAADAKRFELSDLRYRNGVASYLDLLDAQRSLFTSEQLVVQTRLLQLQNQVALYKVLGGGWTAAQEPP
ATGGGATAAPHS
>Mature_492_residues
MQAPSRARLGARPLLCAAAAAALLAGCSFIPTYERPAAPVPQAYPAAGAAAAPAGARAAADIDWREYFADPRLQRLIALA
LENNRDLRVAALNIEQARAQFQIQRAGQFPTVNAAVNASRQPSATTGSYVNNYQVGLAVSAWEIDFFGRIAALKEQALAQ
YFATEEARRATQISLVAAVASGWFNLLADEELLDISRRTLQTRDESVQLTRLRLENGVSSELDYSQAQGLAQAARATFAQ
QQRQRLQDENALALLLGQPLPQEIAATLDARTRLADAPAMPSLPAGLPSDLLVRRPDIRQAEQQLVAANANIGAARAAFF
PRISLTASAGTVSNELSGLFKGGSWAFSLAPQLALPIFDGGRNQATLDSARVGRDIAVAQYEKSIQTAFREVSDALAGQA
TLGEQFAAQRAQAAADAKRFELSDLRYRNGVASYLDLLDAQRSLFTSEQLVVQTRLLQLQNQVALYKVLGGGWTAAQEPP
ATGGGATAAPHS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=40.4301075268817, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI2367348, Length=491, Percent_Identity=25.4582484725051, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52520; Mature: 52520

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPSRARLGARPLLCAAAAAALLAGCSFIPTYERPAAPVPQAYPAAGAAAAPAGARAAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
DIDWREYFADPRLQRLIALALENNRDLRVAALNIEQARAQFQIQRAGQFPTVNAAVNASR
CCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHCCCC
QPSATTGSYVNNYQVGLAVSAWEIDFFGRIAALKEQALAQYFATEEARRATQISLVAAVA
CCCCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGWFNLLADEELLDISRRTLQTRDESVQLTRLRLENGVSSELDYSQAQGLAQAARATFAQ
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
QQRQRLQDENALALLLGQPLPQEIAATLDARTRLADAPAMPSLPAGLPSDLLVRRPDIRQ
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHH
AEQQLVAANANIGAARAAFFPRISLTASAGTVSNELSGLFKGGSWAFSLAPQLALPIFDG
HHHHHHHCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCHHCCEEECC
GRNQATLDSARVGRDIAVAQYEKSIQTAFREVSDALAGQATLGEQFAAQRAQAAADAKRF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELSDLRYRNGVASYLDLLDAQRSLFTSEQLVVQTRLLQLQNQVALYKVLGGGWTAAQEPP
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCCCCC
ATGGGATAAPHS
CCCCCCCCCCCC
>Mature Secondary Structure
MQAPSRARLGARPLLCAAAAAALLAGCSFIPTYERPAAPVPQAYPAAGAAAAPAGARAAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
DIDWREYFADPRLQRLIALALENNRDLRVAALNIEQARAQFQIQRAGQFPTVNAAVNASR
CCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHCCCC
QPSATTGSYVNNYQVGLAVSAWEIDFFGRIAALKEQALAQYFATEEARRATQISLVAAVA
CCCCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGWFNLLADEELLDISRRTLQTRDESVQLTRLRLENGVSSELDYSQAQGLAQAARATFAQ
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
QQRQRLQDENALALLLGQPLPQEIAATLDARTRLADAPAMPSLPAGLPSDLLVRRPDIRQ
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHH
AEQQLVAANANIGAARAAFFPRISLTASAGTVSNELSGLFKGGSWAFSLAPQLALPIFDG
HHHHHHHCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCHHCCEEECC
GRNQATLDSARVGRDIAVAQYEKSIQTAFREVSDALAGQATLGEQFAAQRAQAAADAKRF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELSDLRYRNGVASYLDLLDAQRSLFTSEQLVVQTRLLQLQNQVALYKVLGGGWTAAQEPP
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCCCCC
ATGGGATAAPHS
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]