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The map label for this gene is pckG [H]

Identifier: 120608846

GI number: 120608846

Start: 147863

End: 149725

Strand: Reverse

Name: pckG [H]

Synonym: Aave_0138

Alternate gene names: 120608846

Gene position: 149725-147863 (Counterclockwise)

Preceding gene: 120608847

Following gene: 120608844

Centisome position: 2.8

GC content: 66.67

Gene sequence:

>1863_bases
ATGAACGCACCCACCATGCAAGGTCTCGACCTCCAGGCCCCCGCATACGTCAAGAACGCCCGCCTGCTGGCCTGGGTGGC
CGACATGGCCGCCCTCTGCAAGCCCGCCAGGATCCATTGGTGCGACGGCTCGCAGGAAGAGTACGACCGCCTCTGCCAGC
AACTGGTGGATGCCGGCACCTTCAAGAAGCTCAATCCCGCCAAGCGCCCCGGCAGCTACCTGGCCTGGTCCGACCCGTCG
GACGTGGCCCGCGTGGAAGACCGCACCTACATCTGCTGCGAGAAGAAGGAAGACGCCGGCCCGACCAACAACTGGATGGC
CCCGGCCGAGATGCGCGCCACGCTGCAGCCGCTGTTCGACGGCTGCATGGCCGGCCGCACGATGTACGTCGTGCCCTTCA
GCATGGGCCCGCTGGGCTCGCCCATCGCCCACATCGGCATCGAACTCTCCGACAGCCCCTACGTGGCCGTCAACATGAAG
ATCATGACCCGCATGGGCCGTGCCGTGTACGACGTGCTGGGCGTGGAAGGCGACTTCGTGCCCTGCGTGCACACCGTGGG
CGCGCCCCTGCAGCCCGGCCAGCAGGACGTGTCCTGGCCCTGCAACAAGACCAAGTACATCGTGCATTACCCCGAGACGC
GCGAGATCTGGTCGTACGGCTCCGGCTACGGCGGCAACGCGCTGCTGGGCAAGAAGTGCTTCGCGCTGCGCATCGCCTCC
ACCATGGGCCGTGACCAGGGCTGGCTGGCCGAGCACATGCTGATCCTGGGCGTTTCCAGCCCCGAAGGCAAGAAGTACCA
CGTGGCCGCTGCATTCCCCAGCGCCTGCGGCAAGACCAACTTCTCCATGCTGGTGCCCCCGAAGGCCTTCGAAGGCTGGA
AGGTCACCACGATCGGCGACGACATCGCCTGGATCAAGCCCCAGGCCGACGGCAGCCTGCGCGCCATCAACCCCGAAGCC
GGCTACTTCGGCGTGGCCCCGGGCACCAACTACCACACCAACCCCAACTGCATGGCCAGCCTCGACCGTGACGTGATCTT
CACGAACGTCGCGCTGACCGACGACGGCGACGTGTGGTGGGAAGGCATGGAAAAGGACACCGGCAAGCTGCCGGACCACC
TGATCGACTGGCAGGGCAAGGACTGGACGCCCCAGATCGCCAAGGAAACCGGCGCCAAGGCCGCCCACCCCAACGCCCGC
TTCACCGTGGCCGCCACCAACAACCCCGCGCTGGACCAAGCCTGGGACGATCCCAAGGGCGTGAAGATCGACGCCTTCAT
CTTCGGCGGCCGCCGCTCCACCACCGTGCCGCTGGTGACCGAGGCGCGCGACTGGACCGAGGGCGTGTACATGGCCGCCA
CCATGGGCTCCGAAACCACCGCCGCCGCCTTCGGCGCGCAGGGCGTGGTGCGCCGCGACCCGTTCGCCATGCTGCCCTTC
ATGGGCTACAACATGAGCGACTACTTCCAGCACTGGCTCAACCTGGGCGAGAAGCTCTCCGCCTCGGGCGCCACTCTGCC
GCGCATCTACACGACCAACTGGTTCCGCAAGGATGCCGAAGGCAAGTTCGTCTGGCCCGGCTACGGCGAGAACATGCGCG
TGCTCAAGTGGATGATCGACCGCATCGAAGGCCGCGCCACCGGCCAGGAAACCGCCTTCGGCGTGGCGCCGCAATACGGC
GAGATCAACTGGACGGGCCTGGACTTCTCGAGCGAGCAGTTCGACACCGTGACCCGCATCGACAAGGCCGCCTGGGCCGA
AGAGCTGAAGCTGCACACGGCGCATTTCGACCAACTCGCCTACCATCTCCCGGAGCAGATGCTCAAGACCAAGGCAGCGC
TGGAAAAGCGCCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
AAGACGTTTTCTCAAAGACAGCGCTCAGGATCCAGCGCGGCCTTGCCGCATCCCGTCCGCTTCCTTTGAAAAACCGTTTT
TCAACTTCAGGAAGCCCGCG

Downstream 100 bases:

>100_bases
TTTCCTCGCGGCCCCGGCTCGCCCGCCACGTGCGGACAATTGTCCGCCAGCCCAGCGAAAGCCCCGCGCCACGCCCGTGG
CCGGGGCTTTTTGCTGGCTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MNAPTMQGLDLQAPAYVKNARLLAWVADMAALCKPARIHWCDGSQEEYDRLCQQLVDAGTFKKLNPAKRPGSYLAWSDPS
DVARVEDRTYICCEKKEDAGPTNNWMAPAEMRATLQPLFDGCMAGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMK
IMTRMGRAVYDVLGVEGDFVPCVHTVGAPLQPGQQDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
TMGRDQGWLAEHMLILGVSSPEGKKYHVAAAFPSACGKTNFSMLVPPKAFEGWKVTTIGDDIAWIKPQADGSLRAINPEA
GYFGVAPGTNYHTNPNCMASLDRDVIFTNVALTDDGDVWWEGMEKDTGKLPDHLIDWQGKDWTPQIAKETGAKAAHPNAR
FTVAATNNPALDQAWDDPKGVKIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAFGAQGVVRRDPFAMLPF
MGYNMSDYFQHWLNLGEKLSASGATLPRIYTTNWFRKDAEGKFVWPGYGENMRVLKWMIDRIEGRATGQETAFGVAPQYG
EINWTGLDFSSEQFDTVTRIDKAAWAEELKLHTAHFDQLAYHLPEQMLKTKAALEKRLAA

Sequences:

>Translated_620_residues
MNAPTMQGLDLQAPAYVKNARLLAWVADMAALCKPARIHWCDGSQEEYDRLCQQLVDAGTFKKLNPAKRPGSYLAWSDPS
DVARVEDRTYICCEKKEDAGPTNNWMAPAEMRATLQPLFDGCMAGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMK
IMTRMGRAVYDVLGVEGDFVPCVHTVGAPLQPGQQDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
TMGRDQGWLAEHMLILGVSSPEGKKYHVAAAFPSACGKTNFSMLVPPKAFEGWKVTTIGDDIAWIKPQADGSLRAINPEA
GYFGVAPGTNYHTNPNCMASLDRDVIFTNVALTDDGDVWWEGMEKDTGKLPDHLIDWQGKDWTPQIAKETGAKAAHPNAR
FTVAATNNPALDQAWDDPKGVKIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAFGAQGVVRRDPFAMLPF
MGYNMSDYFQHWLNLGEKLSASGATLPRIYTTNWFRKDAEGKFVWPGYGENMRVLKWMIDRIEGRATGQETAFGVAPQYG
EINWTGLDFSSEQFDTVTRIDKAAWAEELKLHTAHFDQLAYHLPEQMLKTKAALEKRLAA
>Mature_620_residues
MNAPTMQGLDLQAPAYVKNARLLAWVADMAALCKPARIHWCDGSQEEYDRLCQQLVDAGTFKKLNPAKRPGSYLAWSDPS
DVARVEDRTYICCEKKEDAGPTNNWMAPAEMRATLQPLFDGCMAGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMK
IMTRMGRAVYDVLGVEGDFVPCVHTVGAPLQPGQQDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
TMGRDQGWLAEHMLILGVSSPEGKKYHVAAAFPSACGKTNFSMLVPPKAFEGWKVTTIGDDIAWIKPQADGSLRAINPEA
GYFGVAPGTNYHTNPNCMASLDRDVIFTNVALTDDGDVWWEGMEKDTGKLPDHLIDWQGKDWTPQIAKETGAKAAHPNAR
FTVAATNNPALDQAWDDPKGVKIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAFGAQGVVRRDPFAMLPF
MGYNMSDYFQHWLNLGEKLSASGATLPRIYTTNWFRKDAEGKFVWPGYGENMRVLKWMIDRIEGRATGQETAFGVAPQYG
EINWTGLDFSSEQFDTVTRIDKAAWAEELKLHTAHFDQLAYHLPEQMLKTKAALEKRLAA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=631, Percent_Identity=46.7511885895404, Blast_Score=577, Evalue=1e-164,
Organism=Homo sapiens, GI66346721, Length=610, Percent_Identity=48.8524590163934, Blast_Score=571, Evalue=1e-163,
Organism=Homo sapiens, GI66346723, Length=381, Percent_Identity=52.49343832021, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71989645, Length=622, Percent_Identity=45.4983922829582, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71989658, Length=622, Percent_Identity=45.4983922829582, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17555492, Length=625, Percent_Identity=47.36, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71989653, Length=599, Percent_Identity=46.4106844741235, Blast_Score=532, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI32564411, Length=558, Percent_Identity=49.1039426523297, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32564407, Length=552, Percent_Identity=48.3695652173913, Blast_Score=492, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71989649, Length=555, Percent_Identity=45.2252252252252, Blast_Score=473, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI32564409, Length=378, Percent_Identity=45.5026455026455, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17540956, Length=551, Percent_Identity=25.2268602540835, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24655085, Length=607, Percent_Identity=49.0939044481054, Blast_Score=588, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=48.1054365733114, Blast_Score=568, Evalue=1e-162,
Organism=Drosophila melanogaster, GI161077226, Length=564, Percent_Identity=49.290780141844, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24655088, Length=511, Percent_Identity=50.6849315068493, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68534; Mature: 68534

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTMQGLDLQAPAYVKNARLLAWVADMAALCKPARIHWCDGSQEEYDRLCQQLVDAGT
CCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCC
FKKLNPAKRPGSYLAWSDPSDVARVEDRTYICCEKKEDAGPTNNWMAPAEMRATLQPLFD
CCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GCMAGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMKIMTRMGRAVYDVLGVEGDFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
PCVHTVGAPLQPGQQDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHHHHHH
TMGRDQGWLAEHMLILGVSSPEGKKYHVAAAFPSACGKTNFSMLVPPKAFEGWKVTTIGD
HCCCCCCHHHEEEEEEEECCCCCCEEEEEEECCHHCCCCCCEEEECCCCCCCEEEEEECC
DIAWIKPQADGSLRAINPEAGYFGVAPGTNYHTNPNCMASLDRDVIFTNVALTDDGDVWW
CEEEECCCCCCCEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCEE
EGMEKDTGKLPDHLIDWQGKDWTPQIAKETGAKAAHPNARFTVAATNNPALDQAWDDPKG
CCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
VKIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAFGAQGVVRRDPFAMLPF
CEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCEECCC
MGYNMSDYFQHWLNLGEKLSASGATLPRIYTTNWFRKDAEGKFVWPGYGENMRVLKWMID
CCCCHHHHHHHHHCCCHHCCCCCCCCCEEEECCCEEECCCCCEECCCCCCCHHHHHHHHH
RIEGRATGQETAFGVAPQYGEINWTGLDFSSEQFDTVTRIDKAAWAEELKLHTAHFDQLA
HHCCCCCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHLPEQMLKTKAALEKRLAA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAPTMQGLDLQAPAYVKNARLLAWVADMAALCKPARIHWCDGSQEEYDRLCQQLVDAGT
CCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCC
FKKLNPAKRPGSYLAWSDPSDVARVEDRTYICCEKKEDAGPTNNWMAPAEMRATLQPLFD
CCCCCCCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GCMAGRTMYVVPFSMGPLGSPIAHIGIELSDSPYVAVNMKIMTRMGRAVYDVLGVEGDFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
PCVHTVGAPLQPGQQDVSWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHHHHHH
TMGRDQGWLAEHMLILGVSSPEGKKYHVAAAFPSACGKTNFSMLVPPKAFEGWKVTTIGD
HCCCCCCHHHEEEEEEEECCCCCCEEEEEEECCHHCCCCCCEEEECCCCCCCEEEEEECC
DIAWIKPQADGSLRAINPEAGYFGVAPGTNYHTNPNCMASLDRDVIFTNVALTDDGDVWW
CEEEECCCCCCCEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCEE
EGMEKDTGKLPDHLIDWQGKDWTPQIAKETGAKAAHPNARFTVAATNNPALDQAWDDPKG
CCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
VKIDAFIFGGRRSTTVPLVTEARDWTEGVYMAATMGSETTAAAFGAQGVVRRDPFAMLPF
CEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCEECCC
MGYNMSDYFQHWLNLGEKLSASGATLPRIYTTNWFRKDAEGKFVWPGYGENMRVLKWMID
CCCCHHHHHHHHHCCCHHCCCCCCCCCEEEECCCEEECCCCCEECCCCCCCHHHHHHHHH
RIEGRATGQETAFGVAPQYGEINWTGLDFSSEQFDTVTRIDKAAWAEELKLHTAHFDQLA
HHCCCCCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHLPEQMLKTKAALEKRLAA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA