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The map label for this gene is nccB [H]

Identifier: 120608752

GI number: 120608752

Start: 45050

End: 46261

Strand: Reverse

Name: nccB [H]

Synonym: Aave_0039

Alternate gene names: 120608752

Gene position: 46261-45050 (Counterclockwise)

Preceding gene: 120608753

Following gene: 120608751

Centisome position: 0.86

GC content: 73.93

Gene sequence:

>1212_bases
ATGATGGCTCCCCTTCTCTCCCTTCCCGCGCGGCGTCCTTCCGCGCGCGGCCTGCTGGCCCTGGCCGCGCTCGGCGCCGC
CTGCGCGCTGCTGCCCGGCTGCGGCAAGTCCGGCGCGGAAGCCGCCGCCACGCCGCCCGAACCCGCCCCGCCCGTGATGC
AGGCCGGCCAGCTGCGCTTTCCGTCCGGCCATCCGCAACTCGCCCTGCTGGGCACCGTGGAAGCCCGTGCCGCCAGGGAC
GTGGCGGTGGACCTGCCCGCCCGCCTGGTGTGGAACGAAGAGCGCACCCAGCGCATCTACCCCGCCTTCGCCGGCCGCGT
GACCGCCATCCAGGCCGACCTGGGCCAATCGGTCAAGGCCGGCGCGCCCCTCGCCCTGCTGGCCTCGCCCGACTTCGGCC
AGGCCCAGGCCGACACCGCCAAGGCCCAGGCCGGGCAATCGCTGGCGCAGCAATCGCTCAAGCGCCAGCGCGAGCTGTTC
GAGGCCGGCATCATCGCCCGCAAGGACCTGGAGCAGGCCGAGGCCGACGCCGCCGGAGCCCAGGCCGAAGTGGCCCGCGC
CGCGGCCCGCACGCGCCTCTACGGCAGCGCCAGCGGCGTGAACCAGCAACTGGCCCTCTCCACCGGCATCGGCGGCGTGG
TGGTCGAGCGCAACCTCAACCCCGGCCAGGAAGTGCGGCCCGACCAGTCCGGCCCGGCGCTCTTCGTGGTGACCGACCCG
ACCTCCCTCTGGGTGCAGATCGACGCGCGCGAAACCGACCTGTCGTCCCTGCGCCCGGGCGACCCGATCACGCTGCAGGT
GCCGGCCTACCCCGGAGCCACCTTCGCCGGCAAGGTCACGGCCACGGCCGATGCCATCGATCCGGGCACGCGCACGATCA
AGGTGCGGGGCGTGGTGCCCAACGCCGACCGCCGCCTGAAGGCCGAGATGCTGGCCAGCGCCCGCGTGCAGGAAAGCCGC
GCCGGCGGCGTGGTGGTGCCCGCCAGCGCCATCGTGCTGGACGGCACGCAGCACCTCGTCTTCGTGCGCCGCGAGTCCGG
CGTGTTCGAGCCGCGCAAGGTCGAACTGGCCCATGAAGGCCCCGCCGAAGTGCTCGTTTCCGCCGGCCTGCAGCCCGGCG
AAGCCGTGGTGAACCAGAACGCGCTGCTGCTGGCGCGCCAGTTCCAGTCCATGGCCGAAGACGCCGCCACCGCGAAGAAA
GAGGCCCCATGA

Upstream 100 bases:

>100_bases
CCCGCAGTTGCTCACCGGCACGCCCTGACCCCGGCCTGAACGCCGCGCCACAGCACTGCCTTCCCGCCTTCCACCGCATT
CCTTTCCGGACCTCCCGACC

Downstream 100 bases:

>100_bases
AGCGTCTCATCCACTACGCGCTGCACCAGCCCCTGTTCATCATCCTGGGCGTGCTGCTGTTCGCGCTCGCGGGCGTGATC
GCCTTCAAGAACCTGTCGGT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MMAPLLSLPARRPSARGLLALAALGAACALLPGCGKSGAEAAATPPEPAPPVMQAGQLRFPSGHPQLALLGTVEARAARD
VAVDLPARLVWNEERTQRIYPAFAGRVTAIQADLGQSVKAGAPLALLASPDFGQAQADTAKAQAGQSLAQQSLKRQRELF
EAGIIARKDLEQAEADAAGAQAEVARAAARTRLYGSASGVNQQLALSTGIGGVVVERNLNPGQEVRPDQSGPALFVVTDP
TSLWVQIDARETDLSSLRPGDPITLQVPAYPGATFAGKVTATADAIDPGTRTIKVRGVVPNADRRLKAEMLASARVQESR
AGGVVVPASAIVLDGTQHLVFVRRESGVFEPRKVELAHEGPAEVLVSAGLQPGEAVVNQNALLLARQFQSMAEDAATAKK
EAP

Sequences:

>Translated_403_residues
MMAPLLSLPARRPSARGLLALAALGAACALLPGCGKSGAEAAATPPEPAPPVMQAGQLRFPSGHPQLALLGTVEARAARD
VAVDLPARLVWNEERTQRIYPAFAGRVTAIQADLGQSVKAGAPLALLASPDFGQAQADTAKAQAGQSLAQQSLKRQRELF
EAGIIARKDLEQAEADAAGAQAEVARAAARTRLYGSASGVNQQLALSTGIGGVVVERNLNPGQEVRPDQSGPALFVVTDP
TSLWVQIDARETDLSSLRPGDPITLQVPAYPGATFAGKVTATADAIDPGTRTIKVRGVVPNADRRLKAEMLASARVQESR
AGGVVVPASAIVLDGTQHLVFVRRESGVFEPRKVELAHEGPAEVLVSAGLQPGEAVVNQNALLLARQFQSMAEDAATAKK
EAP
>Mature_403_residues
MMAPLLSLPARRPSARGLLALAALGAACALLPGCGKSGAEAAATPPEPAPPVMQAGQLRFPSGHPQLALLGTVEARAARD
VAVDLPARLVWNEERTQRIYPAFAGRVTAIQADLGQSVKAGAPLALLASPDFGQAQADTAKAQAGQSLAQQSLKRQRELF
EAGIIARKDLEQAEADAAGAQAEVARAAARTRLYGSASGVNQQLALSTGIGGVVVERNLNPGQEVRPDQSGPALFVVTDP
TSLWVQIDARETDLSSLRPGDPITLQVPAYPGATFAGKVTATADAIDPGTRTIKVRGVVPNADRRLKAEMLASARVQESR
AGGVVVPASAIVLDGTQHLVFVRRESGVFEPRKVELAHEGPAEVLVSAGLQPGEAVVNQNALLLARQFQSMAEDAATAKK
EAP

Specific function: Component of the NCC cation efflux system that confers resistance to nickel, cobalt and cadmium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=301, Percent_Identity=24.5847176079734, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41846; Mature: 41846

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAPLLSLPARRPSARGLLALAALGAACALLPGCGKSGAEAAATPPEPAPPVMQAGQLRF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCEEC
PSGHPQLALLGTVEARAARDVAVDLPARLVWNEERTQRIYPAFAGRVTAIQADLGQSVKA
CCCCCCEEEEEECHHHHHCCEEECCCHHEEECCHHHHHHHHHHCCEEEEEEHHHCCCCCC
GAPLALLASPDFGQAQADTAKAQAGQSLAQQSLKRQRELFEAGIIARKDLEQAEADAAGA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QAEVARAAARTRLYGSASGVNQQLALSTGIGGVVVERNLNPGQEVRPDQSGPALFVVTDP
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECC
TSLWVQIDARETDLSSLRPGDPITLQVPAYPGATFAGKVTATADAIDPGTRTIKVRGVVP
CEEEEEEECCCCCHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEECC
NADRRLKAEMLASARVQESRAGGVVVPASAIVLDGTQHLVFVRRESGVFEPRKVELAHEG
CCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCCCCCEEEECCCC
PAEVLVSAGLQPGEAVVNQNALLLARQFQSMAEDAATAKKEAP
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMAPLLSLPARRPSARGLLALAALGAACALLPGCGKSGAEAAATPPEPAPPVMQAGQLRF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCEEC
PSGHPQLALLGTVEARAARDVAVDLPARLVWNEERTQRIYPAFAGRVTAIQADLGQSVKA
CCCCCCEEEEEECHHHHHCCEEECCCHHEEECCHHHHHHHHHHCCEEEEEEHHHCCCCCC
GAPLALLASPDFGQAQADTAKAQAGQSLAQQSLKRQRELFEAGIIARKDLEQAEADAAGA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QAEVARAAARTRLYGSASGVNQQLALSTGIGGVVVERNLNPGQEVRPDQSGPALFVVTDP
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEECC
TSLWVQIDARETDLSSLRPGDPITLQVPAYPGATFAGKVTATADAIDPGTRTIKVRGVVP
CEEEEEEECCCCCHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEECC
NADRRLKAEMLASARVQESRAGGVVVPASAIVLDGTQHLVFVRRESGVFEPRKVELAHEG
CCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCCCCCEEEECCCC
PAEVLVSAGLQPGEAVVNQNALLLARQFQSMAEDAATAKKEAP
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]