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The map label for this gene is iciA [H]

Identifier: 120599131

GI number: 120599131

Start: 2681454

End: 2682383

Strand: Direct

Name: iciA [H]

Synonym: Sputw3181_2327

Alternate gene names: 120599131

Gene position: 2681454-2682383 (Clockwise)

Preceding gene: 120599129

Following gene: 120599134

Centisome position: 56.95

GC content: 46.13

Gene sequence:

>930_bases
ATGATGTTGGAATACGCCAATCTTAAAGCACTTGCTGTCGTTGTCTCTGAAGGTGGCTTTGAGCGTGCCGCTAAAGTGCT
GCATATCACGCAATCGGCTGTCTCACAACGCGTACGGCAGTTGGAGGAAAGAGTCGGTCAATCCCTACTGATTCGCTCAA
ATCCTGTCGTGGCAACACCGACAGGTAAACGCTTATTACGCCATTTTTCACAGGTACAACTATTGGAAAGTGAGTTAAGG
GCTGAATTAGATGCCGATGATCCTAATCACCCCACAACGGTCCGCATCGCGGTAAATGCAGACAGTTTAGCAACCTGGTT
CTTACCCGCGCTTTCAGGCATGTTTAGCCGCCGTGCTTGGCTCTTGGAATTAATTGTCGATGATGAATCTTACACCCACC
ATTTACTTAAAAACGGTGAAGCTGTGGGCTGCGTAACAACGACAGAGAGCCCTATGGCTGGTTGTAGCAGTGAATTTTTA
GGCACGATGGAATATCTCTGTGTGGCAACGCCCGAATTTGCCGCCCGTTACTTTAGTAATGGCAATACTGTAAATCCCCC
CAATAATCCAATGATGCTCACCCCTGATCAACTCACTAAGGCCCCAGCGGTTGTCTTTTCCACTAAGGATAAACTCCATG
AAAAGTATCTTGCTAAGTATTTTCAGATGACACCAGGACAATGGTGGCAGCACACCATTCCCTCATCGGAGAGCTTTTTA
GAAGCCATTAACTTGAGTTTAGGCTATGGTCTTGTTGGACATTTACAAGCTAAGCCATTAATAGATAAAGGTGTATTGAT
TGAACTCACACCTAACTGTCGCATGCCTGTACCCTTGTTTTGGCAACATTGGAATATCAAAGCCAAACAAACCACTTTGG
TTTATCGTGCTCTGGCGGCCACGGCGCAACACCATTTATTGCAGCAATAA

Upstream 100 bases:

>100_bases
ATGTTTGAAGAAGGCGCAAGCATAGTCAAAACAACAAAATTAATATAACTAATGTTTTTTATGTATCATAAGTAAAATTA
ATAATGATTAGACTGGAATA

Downstream 100 bases:

>100_bases
GTCATATTATTGAAGTGGATAAACAAAAAACTGAGCGTTCTGCTCAGTTTTTAATCGCTGTTAGTCATGTTACAACGAGA
TTATGCTTGCGGGGGAGCGG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MMLEYANLKALAVVVSEGGFERAAKVLHITQSAVSQRVRQLEERVGQSLLIRSNPVVATPTGKRLLRHFSQVQLLESELR
AELDADDPNHPTTVRIAVNADSLATWFLPALSGMFSRRAWLLELIVDDESYTHHLLKNGEAVGCVTTTESPMAGCSSEFL
GTMEYLCVATPEFAARYFSNGNTVNPPNNPMMLTPDQLTKAPAVVFSTKDKLHEKYLAKYFQMTPGQWWQHTIPSSESFL
EAINLSLGYGLVGHLQAKPLIDKGVLIELTPNCRMPVPLFWQHWNIKAKQTTLVYRALAATAQHHLLQQ

Sequences:

>Translated_309_residues
MMLEYANLKALAVVVSEGGFERAAKVLHITQSAVSQRVRQLEERVGQSLLIRSNPVVATPTGKRLLRHFSQVQLLESELR
AELDADDPNHPTTVRIAVNADSLATWFLPALSGMFSRRAWLLELIVDDESYTHHLLKNGEAVGCVTTTESPMAGCSSEFL
GTMEYLCVATPEFAARYFSNGNTVNPPNNPMMLTPDQLTKAPAVVFSTKDKLHEKYLAKYFQMTPGQWWQHTIPSSESFL
EAINLSLGYGLVGHLQAKPLIDKGVLIELTPNCRMPVPLFWQHWNIKAKQTTLVYRALAATAQHHLLQQ
>Mature_309_residues
MMLEYANLKALAVVVSEGGFERAAKVLHITQSAVSQRVRQLEERVGQSLLIRSNPVVATPTGKRLLRHFSQVQLLESELR
AELDADDPNHPTTVRIAVNADSLATWFLPALSGMFSRRAWLLELIVDDESYTHHLLKNGEAVGCVTTTESPMAGCSSEFL
GTMEYLCVATPEFAARYFSNGNTVNPPNNPMMLTPDQLTKAPAVVFSTKDKLHEKYLAKYFQMTPGQWWQHTIPSSESFL
EAINLSLGYGLVGHLQAKPLIDKGVLIELTPNCRMPVPLFWQHWNIKAKQTTLVYRALAATAQHHLLQQ

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=306, Percent_Identity=37.9084967320261, Blast_Score=190, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34493; Mature: 34493

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLEYANLKALAVVVSEGGFERAAKVLHITQSAVSQRVRQLEERVGQSLLIRSNPVVATP
CEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECC
TGKRLLRHFSQVQLLESELRAELDADDPNHPTTVRIAVNADSLATWFLPALSGMFSRRAW
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHE
LLELIVDDESYTHHLLKNGEAVGCVTTTESPMAGCSSEFLGTMEYLCVATPEFAARYFSN
EEEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCC
GNTVNPPNNPMMLTPDQLTKAPAVVFSTKDKLHEKYLAKYFQMTPGQWWQHTIPSSESFL
CCCCCCCCCCEEECHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHH
EAINLSLGYGLVGHLQAKPLIDKGVLIELTPNCRMPVPLFWQHWNIKAKQTTLVYRALAA
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
TAQHHLLQQ
HHHHHHHCC
>Mature Secondary Structure
MMLEYANLKALAVVVSEGGFERAAKVLHITQSAVSQRVRQLEERVGQSLLIRSNPVVATP
CEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECC
TGKRLLRHFSQVQLLESELRAELDADDPNHPTTVRIAVNADSLATWFLPALSGMFSRRAW
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHE
LLELIVDDESYTHHLLKNGEAVGCVTTTESPMAGCSSEFLGTMEYLCVATPEFAARYFSN
EEEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCC
GNTVNPPNNPMMLTPDQLTKAPAVVFSTKDKLHEKYLAKYFQMTPGQWWQHTIPSSESFL
CCCCCCCCCCEEECHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHH
EAINLSLGYGLVGHLQAKPLIDKGVLIELTPNCRMPVPLFWQHWNIKAKQTTLVYRALAA
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
TAQHHLLQQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9286976 [H]