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The map label for this gene is cmoB [H]

Identifier: 120598884

GI number: 120598884

Start: 2388074

End: 2389072

Strand: Direct

Name: cmoB [H]

Synonym: Sputw3181_2076

Alternate gene names: 120598884

Gene position: 2388074-2389072 (Clockwise)

Preceding gene: 120598883

Following gene: 120598885

Centisome position: 50.72

GC content: 48.25

Gene sequence:

>999_bases
GTGATCAGCTTTAGCTCGTTTTACCAACAAATCGCCGATTCCAATCTACAACACTGGCTCGAAACCCTGCCGAGCATTTT
AGGCAAGTGGCAACGAGATCATAAACACGGTAATTTGCCCAAGTGGGAAAAAGTGCTCAACAAGCTGCACTATCCAGCAC
CCGACCAAGTGGATTTTGTCGATAGCGTCACCGTCGGCAGCGGCGAGCAACTCAGTCCTGGCGAGAAAGAGAAGCTAGAG
AATCTACTGCGCCTGTTTATGCCTTGGCGCAAAGGGCCCTTCCACATTCACGGCATTCATATCGATACCGAATGGCGCAG
TGACTGGAAATGGGATCGCGTTAAGCAGCATATTTCACCGCTAAACAATCGCACAGTGCTTGATGTTGGTTGTGGCAGCG
GTTATCACATGTGGCGCATGTTAGGTGCAGGTGCTAAACGCGTAGTGGGCATCGACCCTTCGCCGTTGTTTTTATGTCAG
TTTGAAGCGGTAAAACGTTTAGCGGGCGCCCATCATCCTGTGCACCTTTTGCCACTGGGTATTGAAGAATTACCACCGCT
GGATGCATTCGACACGGTTTTCTCTATGGGCGTCTTGTATCACAGACGTTCGCCTATCGATCATTTACTGCAATTGCGTG
ATCAGTTACGTACTGGCGGTGAACTCGTGCTAGAAACCTTGGTGATAGATGGCGACGAAAACGCCGTACTCGTGCCTCAA
GATAGATATGGCAAGATGAATAATGTATGGTTTATTCCGTCAGTTGCCGCGCTCATGCTCTGGTTAAAGAAATGTGATTT
CACTGATATCCGTTGTGTCGATACCGATGTCACAGCCCTTGCAGAGCAACGACGTACAGACTGGATGCCCAATGAGTCAC
TGCTTGAATATCTTGATCCCACTGATATCACTAAAACAATTGAGGGTTATCCAGCGCCTAAGCGAGCGACGATTATTGCA
ATAAAAAACCAGCCTAACCAAGAAACAGTTAATGGGTAA

Upstream 100 bases:

>100_bases
ACACCTTAACCACGCATCAACAACGACTCACCCGCCAAGGCTTTAGTCACTTTAGTCTCTGGTTCCAATGCTTTAACTTT
GCTTCTATGGTGGCCATCAA

Downstream 100 bases:

>100_bases
ACTCACTTTACTGACACTTGGATTATTGTTTAGCGGATTCATTTATGCCGCGCCAAGCCCTGTGCCATTGCTTGTTGAGC
AGTGTCTACGCTTACCTAGA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MISFSSFYQQIADSNLQHWLETLPSILGKWQRDHKHGNLPKWEKVLNKLHYPAPDQVDFVDSVTVGSGEQLSPGEKEKLE
NLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKQHISPLNNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQ
FEAVKRLAGAHHPVHLLPLGIEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRTGGELVLETLVIDGDENAVLVPQ
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLLEYLDPTDITKTIEGYPAPKRATIIA
IKNQPNQETVNG

Sequences:

>Translated_332_residues
MISFSSFYQQIADSNLQHWLETLPSILGKWQRDHKHGNLPKWEKVLNKLHYPAPDQVDFVDSVTVGSGEQLSPGEKEKLE
NLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKQHISPLNNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQ
FEAVKRLAGAHHPVHLLPLGIEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRTGGELVLETLVIDGDENAVLVPQ
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLLEYLDPTDITKTIEGYPAPKRATIIA
IKNQPNQETVNG
>Mature_332_residues
MISFSSFYQQIADSNLQHWLETLPSILGKWQRDHKHGNLPKWEKVLNKLHYPAPDQVDFVDSVTVGSGEQLSPGEKEKLE
NLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKQHISPLNNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQ
FEAVKRLAGAHHPVHLLPLGIEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRTGGELVLETLVIDGDENAVLVPQ
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLLEYLDPTDITKTIEGYPAPKRATIIA
IKNQPNQETVNG

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=322, Percent_Identity=63.0434782608696, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 37962; Mature: 37962

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFSSFYQQIADSNLQHWLETLPSILGKWQRDHKHGNLPKWEKVLNKLHYPAPDQVDFV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHH
DSVTVGSGEQLSPGEKEKLENLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKQHISP
HCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCC
LNNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQFEAVKRLAGAHHPVHLLPLG
CCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHCCCCCEEEEECC
IEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRTGGELVLETLVIDGDENAVLVPQ
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEHEEEEECCCCEEEECH
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLLEYLDP
HHCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHCCC
TDITKTIEGYPAPKRATIIAIKNQPNQETVNG
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MISFSSFYQQIADSNLQHWLETLPSILGKWQRDHKHGNLPKWEKVLNKLHYPAPDQVDFV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHH
DSVTVGSGEQLSPGEKEKLENLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKQHISP
HCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCC
LNNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQFEAVKRLAGAHHPVHLLPLG
CCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHCCCCCEEEEECC
IEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRTGGELVLETLVIDGDENAVLVPQ
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEHEEEEECCCCEEEECH
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLLEYLDP
HHCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHCCC
TDITKTIEGYPAPKRATIIAIKNQPNQETVNG
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA