Click here to switch to the map view.

The map label for this gene is rimK1 [H]

Identifier: 120598683

GI number: 120598683

Start: 2150703

End: 2151608

Strand: Direct

Name: rimK1 [H]

Synonym: Sputw3181_1868

Alternate gene names: 120598683

Gene position: 2150703-2151608 (Clockwise)

Preceding gene: 120598682

Following gene: 120598684

Centisome position: 45.68

GC content: 45.92

Gene sequence:

>906_bases
ATGAGAATCGCGATCCTATCGCAAGGGCCTGAGCTATATTCAACTAAGCGACTCGTTGAAGCGGCTCAACTACGTGGTCA
TGAAGTACACGTAATTAATCCCCTTGAATGTTATATGAATATCAACATGCGGCAATCGAGTATTCATATTGGTGGGAGAG
AACTTCCTACGTTTGATGCAGTGATCCCCCGTATTGGCGCATCGATCACCTTTTATGGTTCGGCGGTGCTGCGTCAGTTT
GAGATGATGGGGGTCTATGCTTTGAATGATTCCGTTGGCATCTCCCGTTCACGTGACAAATTACGTTCAATGCAGTTGAT
GTCACGTCGTGGAATTGGGTTACCAATTACAGGGTTTGCTAATAAACCCAGTGATATTCCTGACTTAATTGATATGGTGG
GTGGTGCGCCTCTTGTTATCAAATTGCTTGAAGGCACACAAGGAATTGGCGTGGTCTTAGCAGAAACCCGTAAAGCTGCC
GAGAGTGTCATTGAAGCCTTTATGGGCCTTAAAGCCAATATCATGGTGCAAGAATATATTAAAGAAGCAAATGGCGCGGA
TATTCGTTGTTTTGTGCTCGGTGATAAAGTGATTGCCGCCATGAAGCGTCAAGCTATGCCAGGTGAGTTCCGCTCTAACT
TGCACCGTGGTGGTACCGCAAGTTTAGTTAAACTTACGCCTGAAGAGCGCTCCGTTGCTATTCGTGCAGCTAAGACTATG
GGACTGAATGTGGCAGGTGTTGATTTGCTACGCTCGAATCATGGCCCCGTCATAATGGAAGTGAATTCCTCCCCTGGGCT
TGAAGGGATTGAAGGTGCCACCACTAAAGATGTGGCAGGTGCGATTATTGAATTTGTTGAAAAAAATGTGACTAAAGTGA
AAAAGGTGACTCAGGCGCAAGGTTAA

Upstream 100 bases:

>100_bases
TATGATGCTGAGCATTTGCTCAAGCAATAATAGCGATGATTGATTTATCCTTTAGCAGGCGTATTATGCGCTCAAAATTA
AATGGTTGCCGGAGACCAGA

Downstream 100 bases:

>100_bases
ATAGTGTAGGTTGCGTCACATTAAGGCTATCAGTTTTTTCGCTTCCCTATAAATGAATAAGGAGAAAAAGATGAGCTTAA
AATGGACAGATTCCTTAGAT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPTFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIEFVEKNVTKVKKVTQAQG

Sequences:

>Translated_301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPTFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIEFVEKNVTKVKKVTQAQG
>Mature_301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPTFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIEFVEKNVTKVKKVTQAQG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=245, Percent_Identity=28.1632653061224, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI134254462, Length=213, Percent_Identity=29.1079812206573, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787076, Length=291, Percent_Identity=67.3539518900344, Blast_Score=416, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32483; Mature: 32483

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPTFDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHEEEECCEEECEEEECCCCCCHHHH
VIPRIGASITFYGSAVLRQFEMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFA
HHHHHCCEEEEHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEECCCC
NKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAAESVIEAFMGLKANIMVQEYI
CCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
KEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHEEEEEHHHH
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIEFVEKNVTKVKKVTQAQ
CCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPTFDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHEEEECCEEECEEEECCCCCCHHHH
VIPRIGASITFYGSAVLRQFEMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFA
HHHHHCCEEEEHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEECCCC
NKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAAESVIEAFMGLKANIMVQEYI
CCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
KEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHEEEEEHHHH
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIEFVEKNVTKVKKVTQAQ
CCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA