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The map label for this gene is queA

Identifier: 120598341

GI number: 120598341

Start: 1728801

End: 1729838

Strand: Direct

Name: queA

Synonym: Sputw3181_1523

Alternate gene names: 120598341

Gene position: 1728801-1729838 (Clockwise)

Preceding gene: 120598340

Following gene: 120598342

Centisome position: 36.72

GC content: 48.75

Gene sequence:

>1038_bases
ATGCGCGTTGCAGACTTTTCTTTCGATCTCCCCGATGAGCTGATCGCTCGTTATCCTATGGCGCAGCGTAATGCATCCCG
TCTGTTAACGTTAGACGGCAACTCTGGTGCGTTAGGCGACAAACAGTTTACTGATCTGCTGGAAATGATAAATCCCGGCG
ATTTAATGGTGTTCAACAATACTCGCGTGATCCCCGCGCGTATGTTTGGCCAAAAGGCCAGTGGCGGCAAGTTAGAAATT
TTAGTTGAGCGTATGCTCGATGATAAGCGGATCCTTGCCCATGTTCGCAGCTCTAAATCCCCGAAAGTCGATTCGTTAAT
CCATCTCGATGGTGGCTATCAAATGAAGATGGTCGCCCGTCACGATACTTTGTTTGAACTTGAGCTGTTATCCGAGCTGA
CCATTCTAGAGGTGCTTGAAGCAGTAGGGCACATGCCTTTGCCTCCCTATATTGATAGACCCGATGAAGATGCCGACAAA
GAGCGCTATCAAACGGTCTATAACCAAAACCCCGGTGCAGTAGCAGCGCCAACGGCGGGTTTACATTTTGACGATGCCAT
GCTCGATGCATTAAAAGCCAAGGGCGTCAATATTGCCTTTGTGACCTTGCATGTGGGCGCGGGAACATTTCAGCCTGTAC
GTGTCGATACCATTGTTGAGCACAAAATGCATTCCGAGTGGGCCAATGTGCCGCAGGATGTCGTGGATCTAATTGCCCAG
ACTAAGGCGGCGGGTAAACGTGTGGTTGCAGTCGGTACGACTTCTGTGCGTTCACTCGAGAGTGCGGCGCGCGCAAGCCT
TGGCGAACTCAAAGCTTTTAGCGGCGATACCGATATCTTTATTTATCCTGGTTATCAATTCCAAGTGGTTGATGCTATGG
TGACAAATTTCCATCTGCCGGAATCAACTTTGATTATGTTAGTCAGCGCTTTTGCCGGTTTTGATCATGTGATGGCGGCG
TATCAACATGCTATCACGCAAAAATATCGCTTCTTTAGCTATGGCGATGCCATGTTTGTGACGAAAAAAGCCCACTAA

Upstream 100 bases:

>100_bases
ATGGATCACAACAAGAAGTGGACGCGGGTAATAAGTCATCTTGATCTTATGGGGCGCTAACCATAGACTAGCCGCCGTTC
TAAGTTACCCTGAGGCCAAT

Downstream 100 bases:

>100_bases
ACGACGATTTTGTTTTAAAATAGCCGCCCAAATGAGTTTGAGCGGCAATTTTTTATCTATAGGGTTGAACCTTTTAGATC
CGTCCCTATCTCTATTGTTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLEMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLIHLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTIVEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASLGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAITQKYRFFSYGDAMFVTKKAH

Sequences:

>Translated_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLEMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLIHLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTIVEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASLGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAITQKYRFFSYGDAMFVTKKAH
>Mature_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLEMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLIHLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTIVEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASLGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAITQKYRFFSYGDAMFVTKKAH

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=68.6046511627907, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38161; Mature: 38161

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLEMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSLIHLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTIVEHKMHSEWANVPQDVVDLIAQ
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASLGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAITQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEEECCC
>Mature Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLEMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSLIHLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTIVEHKMHSEWANVPQDVVDLIAQ
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASLGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAITQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA