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The map label for this gene is ogt [H]

Identifier: 120598330

GI number: 120598330

Start: 1719224

End: 1719781

Strand: Direct

Name: ogt [H]

Synonym: Sputw3181_1512

Alternate gene names: 120598330

Gene position: 1719224-1719781 (Clockwise)

Preceding gene: 120598329

Following gene: 120598331

Centisome position: 36.51

GC content: 49.28

Gene sequence:

>558_bases
ATGAAGCATGCGATTCGTCAATTGAGCCAAGCGCCGAGTATTGCAGCGTATCAGCCCTGTGCCATGGATACTTTTATGAG
TCCTGTCGGAATATTGCAATTAAATGCCAATGCCTATGGACTAAGTCATTTAACGGTTGTCGGCTCGGATCGCACTGAGA
TTGCGCTTGCTAAGGTGAGTGAGGAGGCCCTCAATCAAGCTAAGGCGCATATTAGTGAAACTCAAGTGCAGCTCGCGCGT
TATTTTGCAGGTGAACGGATTGAATTTAATTTAAAGCTCGCACCTAAGGGCACTGAATTTCAGCGTGAAGTTTGGCAAGC
CTTGCTCAATGTTGATTATGGTCAGAGCTGCAGCTATGGGCAGATTGCCGAGCAAATTGGTCGGCCTAAGGCGGTGCGCG
CCGTGGGCGCGGCCAATGGCGCCAATCCCATCGCGATTATTGTGCCTTGCCATAGAGTAATAGGCAAAAATGGCAAGTTA
ACGGGTTACGCCTACGGGCTGGCGATGAAACAGCAGTTGTTAACGCTGGAAACTGACAAGCACTGCCTCGATATTTAA

Upstream 100 bases:

>100_bases
TCAAGAAATCGCGCCTTGGGGCAGTTATTTAACCTTTCAGCTTTGGCATCAATAGCGACTAAAACGATTTAAGTTGAAGA
CATAAATAGAGAGATAAATT

Downstream 100 bases:

>100_bases
GCTAGAATAGCCGCCTATTAAGATATTGGTGAGCAGTTAAACTTTCTATGACTCAGGCTTCTTCCTCCGTGGCAAGCTTT
GCCCAACTCGGCATTATTGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKHAIRQLSQAPSIAAYQPCAMDTFMSPVGILQLNANAYGLSHLTVVGSDRTEIALAKVSEEALNQAKAHISETQVQLAR
YFAGERIEFNLKLAPKGTEFQREVWQALLNVDYGQSCSYGQIAEQIGRPKAVRAVGAANGANPIAIIVPCHRVIGKNGKL
TGYAYGLAMKQQLLTLETDKHCLDI

Sequences:

>Translated_185_residues
MKHAIRQLSQAPSIAAYQPCAMDTFMSPVGILQLNANAYGLSHLTVVGSDRTEIALAKVSEEALNQAKAHISETQVQLAR
YFAGERIEFNLKLAPKGTEFQREVWQALLNVDYGQSCSYGQIAEQIGRPKAVRAVGAANGANPIAIIVPCHRVIGKNGKL
TGYAYGLAMKQQLLTLETDKHCLDI
>Mature_185_residues
MKHAIRQLSQAPSIAAYQPCAMDTFMSPVGILQLNANAYGLSHLTVVGSDRTEIALAKVSEEALNQAKAHISETQVQLAR
YFAGERIEFNLKLAPKGTEFQREVWQALLNVDYGQSCSYGQIAEQIGRPKAVRAVGAANGANPIAIIVPCHRVIGKNGKL
TGYAYGLAMKQQLLTLETDKHCLDI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=177, Percent_Identity=35.0282485875706, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=58.8235294117647, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788542, Length=91, Percent_Identity=50.5494505494505, Blast_Score=100, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=48.75, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=48.75, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=53.0120481927711, Blast_Score=106, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137554, Length=91, Percent_Identity=47.2527472527472, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20109; Mature: 20109

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAIRQLSQAPSIAAYQPCAMDTFMSPVGILQLNANAYGLSHLTVVGSDRTEIALAKVS
CCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEECCCEECCEEEEEEECCCCEEHHHHHH
EEALNQAKAHISETQVQLARYFAGERIEFNLKLAPKGTEFQREVWQALLNVDYGQSCSYG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHH
QIAEQIGRPKAVRAVGAANGANPIAIIVPCHRVIGKNGKLTGYAYGLAMKQQLLTLETDK
HHHHHHCCCHHHEEECCCCCCCCEEEEEECHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
HCLDI
HHCCC
>Mature Secondary Structure
MKHAIRQLSQAPSIAAYQPCAMDTFMSPVGILQLNANAYGLSHLTVVGSDRTEIALAKVS
CCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEECCCEECCEEEEEEECCCCEEHHHHHH
EEALNQAKAHISETQVQLARYFAGERIEFNLKLAPKGTEFQREVWQALLNVDYGQSCSYG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHH
QIAEQIGRPKAVRAVGAANGANPIAIIVPCHRVIGKNGKLTGYAYGLAMKQQLLTLETDK
HHHHHHCCCHHHEEECCCCCCCCEEEEEECHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
HCLDI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]