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The map label for this gene is vat [H]

Identifier: 120598292

GI number: 120598292

Start: 1660805

End: 1661419

Strand: Direct

Name: vat [H]

Synonym: Sputw3181_1470

Alternate gene names: 120598292

Gene position: 1660805-1661419 (Clockwise)

Preceding gene: 120598291

Following gene: 120598293

Centisome position: 35.27

GC content: 33.33

Gene sequence:

>615_bases
ATGTTTTTTTATTTATTGAAGATGTTTAAAGTTAAATTTTTTTCATCAAGAAATCAAAGACTAAATGATCGTATAACTAC
GATCAGGGGGGAGGGAACTTATGGTCCAGAGCCAAAATTAATTGGGCCTCATAATGTTGTAGAACAATTGCATTTTGGGA
GTTCAATTGGGAAGTATTGCTCTATAGCGGCCGGAATTAAGTTTTTATTTAGAGGTAAACACAATACGAATTGGGTTTCT
ACTTATCCATTCCAAGTAATGATGGGGTTAGATGTTCCTTTAAATGATTTAACACTTCATGCTCCCGTGAAAATTGGTAA
TGATGTCTGGATTGCTACTAATGTAAAAATTATGCAAGGTGTCACTATCGGTGATGGCGCTATAATCGCACAAGAGTCGT
TAGTGACTAAAGATGTTCCTCCATATGCGATTGTTGGTGGAAATCCAGCAAGAATTATAAGATATCGGTTTGAACCTGAT
GAAATTCAAGAATTATTAAATTTACAATGGTGGAATCTACCAAAAAACAAAATTGTGTTGATAGCAAAATATCTAACATC
ACAGGATGTAAATGAGTGCATAAAACAAATAAAAATGGTGAAAGATAATGAATAA

Upstream 100 bases:

>100_bases
CTAGAAAGATTAAAAATCGCAAGCGAGAGTTGTTGGCTGTTGAAAACAATTTCATAGAAAAATAATAAGTGGCTAGTTGA
TACCTGTAAGGTACTATTAT

Downstream 100 bases:

>100_bases
TTCAATGGTCACTGTTTGTATTGTTACGTATAACCAAAAGAAGTATATAGAGCAATGCTTAGATAGCATAGTTAATCAAA
AAACAGACTATTCATATCAA

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MFFYLLKMFKVKFFSSRNQRLNDRITTIRGEGTYGPEPKLIGPHNVVEQLHFGSSIGKYCSIAAGIKFLFRGKHNTNWVS
TYPFQVMMGLDVPLNDLTLHAPVKIGNDVWIATNVKIMQGVTIGDGAIIAQESLVTKDVPPYAIVGGNPARIIRYRFEPD
EIQELLNLQWWNLPKNKIVLIAKYLTSQDVNECIKQIKMVKDNE

Sequences:

>Translated_204_residues
MFFYLLKMFKVKFFSSRNQRLNDRITTIRGEGTYGPEPKLIGPHNVVEQLHFGSSIGKYCSIAAGIKFLFRGKHNTNWVS
TYPFQVMMGLDVPLNDLTLHAPVKIGNDVWIATNVKIMQGVTIGDGAIIAQESLVTKDVPPYAIVGGNPARIIRYRFEPD
EIQELLNLQWWNLPKNKIVLIAKYLTSQDVNECIKQIKMVKDNE
>Mature_204_residues
MFFYLLKMFKVKFFSSRNQRLNDRITTIRGEGTYGPEPKLIGPHNVVEQLHFGSSIGKYCSIAAGIKFLFRGKHNTNWVS
TYPFQVMMGLDVPLNDLTLHAPVKIGNDVWIATNVKIMQGVTIGDGAIIAQESLVTKDVPPYAIVGGNPARIIRYRFEPD
EIQELLNLQWWNLPKNKIVLIAKYLTSQDVNECIKQIKMVKDNE

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=99, Percent_Identity=39.3939393939394, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1786537, Length=53, Percent_Identity=52.8301886792453, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23212; Mature: 23212

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFYLLKMFKVKFFSSRNQRLNDRITTIRGEGTYGPEPKLIGPHNVVEQLHFGSSIGKYC
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEECHHHHHHHHHCCCHHHHHH
SIAAGIKFLFRGKHNTNWVSTYPFQVMMGLDVPLNDLTLHAPVKIGNDVWIATNVKIMQG
HHHHHHEEEEECCCCCCCEEECCEEEEEECCCCCCCEEEEECEEECCEEEEEECEEEEEE
VTIGDGAIIAQESLVTKDVPPYAIVGGNPARIIRYRFEPDEIQELLNLQWWNLPKNKIVL
EEECCCEEEECCCHHCCCCCCEEEECCCCEEEEEEECCHHHHHHHHCCEECCCCCCCEEE
IAKYLTSQDVNECIKQIKMVKDNE
EEEHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFFYLLKMFKVKFFSSRNQRLNDRITTIRGEGTYGPEPKLIGPHNVVEQLHFGSSIGKYC
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEECHHHHHHHHHCCCHHHHHH
SIAAGIKFLFRGKHNTNWVSTYPFQVMMGLDVPLNDLTLHAPVKIGNDVWIATNVKIMQG
HHHHHHEEEEECCCCCCCEEECCEEEEEECCCCCCCEEEEECEEECCEEEEEECEEEEEE
VTIGDGAIIAQESLVTKDVPPYAIVGGNPARIIRYRFEPDEIQELLNLQWWNLPKNKIVL
EEECCCEEEECCCHHCCCCCCEEEECCCCEEEEEEECCHHHHHHHHCCEECCCCCCCEEE
IAKYLTSQDVNECIKQIKMVKDNE
EEEHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]